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Team:MIT mammalian Mechanosensation
From 2010.igem.org
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<b class="bolded" id="overview">Literature Search </b><br> | <b class="bolded" id="overview">Literature Search </b><br> | ||
| - | To begin, we searched the literature for well-characterized mechanosensitive promoters; we restricted our pool of candidates to promoters that had been shown, mostly by upregulation of a fluorescent marker, to be directly responsive to mechanical stress. The nature of the promoters varied. We included several short oligonucleotide sequences SRE/CRE2 and NR1/2, that had been shown to confer sensitivitiy to shear stress when placed in front of a ‘dummy’ SV40 promoter. Promoters for the genes PAI-2 and cfos were identified as mechanosensitive in the literature; we used portions of these promoters which were shown to be responsible for the mechanosensitivity. Click <a href = "https://static.igem.org/mediawiki/2010/e/e1/FocalAdhesionsNotes.pdf">here</a> and <a href = "https://static.igem.org/mediawiki/2010/3/3d/ShearStressNotes.pdf">here</a | + | To begin, we searched the literature for well-characterized mechanosensitive promoters; we restricted our pool of candidates to promoters that had been shown, mostly by upregulation of a fluorescent marker, to be directly responsive to mechanical stress. The nature of the promoters varied. We included several short oligonucleotide sequences SRE/CRE2 and NR1/2, that had been shown to confer sensitivitiy to shear stress when placed in front of a ‘dummy’ SV40 promoter. Promoters for the genes PAI-2 and cfos were identified as mechanosensitive in the literature; we used portions of these promoters which were shown to be responsible for the mechanosensitivity. Click <a href = "https://static.igem.org/mediawiki/2010/e/e1/FocalAdhesionsNotes.pdf">here</a> and <a href = "https://static.igem.org/mediawiki/2010/3/3d/ShearStressNotes.pdf">here</a> for more in-depth notes on our literature review. |
| - | <div class="bodybaby" id="first"> | + | <div class="bodybaby" id="first">Promoter Cloning</div><br> |
</td></table> | </td></table> | ||
Revision as of 21:35, 26 October 2010
iGEM 2010
The Search for Mechanosensitive Promoters |
|
Literature Search To begin, we searched the literature for well-characterized mechanosensitive promoters; we restricted our pool of candidates to promoters that had been shown, mostly by upregulation of a fluorescent marker, to be directly responsive to mechanical stress. The nature of the promoters varied. We included several short oligonucleotide sequences SRE/CRE2 and NR1/2, that had been shown to confer sensitivitiy to shear stress when placed in front of a ‘dummy’ SV40 promoter. Promoters for the genes PAI-2 and cfos were identified as mechanosensitive in the literature; we used portions of these promoters which were shown to be responsible for the mechanosensitivity. Click here and here for more in-depth notes on our literature review. Promoter Cloning |
