Team:MIT mammalian Mechanosensation

From 2010.igem.org

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<b class="bolded" id="overview">Literature Search </b><br>
<b class="bolded" id="overview">Literature Search </b><br>
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To begin, we searched the literature for well-characterized mechanosensitive promoters; we restricted our pool of candidates to promoters that had been shown, mostly by upregulation of a fluorescent marker, to be directly responsive to mechanical stress. The nature of the promoters varied. We included several short oligonucleotide sequences SRE/CRE2 and NR1/2, that had been shown to confer sensitivitiy to shear stress when placed in front of a ‘dummy’ SV40 promoter. Promoters for the genes PAI-2 and cfos were identified as mechanosensitive in the literature; we used portions of these promoters which were shown to be responsible for the mechanosensitivity. Click <a href = "http://2010.igem.org/wiki/images/e/e1/FocalAdhesionsNotes.pdf">here</a> and <a href = "http://2010.igem.org/wiki/images/3/3d/ShearStressNotes.pdf">here</a
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To begin, we searched the literature for well-characterized mechanosensitive promoters; we restricted our pool of candidates to promoters that had been shown, mostly by upregulation of a fluorescent marker, to be directly responsive to mechanical stress. The nature of the promoters varied. We included several short oligonucleotide sequences SRE/CRE2 and NR1/2, that had been shown to confer sensitivitiy to shear stress when placed in front of a ‘dummy’ SV40 promoter. Promoters for the genes PAI-2 and cfos were identified as mechanosensitive in the literature; we used portions of these promoters which were shown to be responsible for the mechanosensitivity. Click <a href = "http://2010.igem.org/wiki/images/e/e1/FocalAdhesionsNotes.pdf">here</a> and <a href = "http://2010.igem.org/wiki/images/3/3d/ShearStressNotes.pdf">here</a> for more in-depth notes on our literature review.
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<div class="bodybaby" id="first">Overview</div><br>
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<div class="bodybaby" id="first">Promoter Cloning</div><br>
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Revision as of 21:35, 26 October 2010

The Search for Mechanosensitive Promoters
Literature Search
To begin, we searched the literature for well-characterized mechanosensitive promoters; we restricted our pool of candidates to promoters that had been shown, mostly by upregulation of a fluorescent marker, to be directly responsive to mechanical stress. The nature of the promoters varied. We included several short oligonucleotide sequences SRE/CRE2 and NR1/2, that had been shown to confer sensitivitiy to shear stress when placed in front of a ‘dummy’ SV40 promoter. Promoters for the genes PAI-2 and cfos were identified as mechanosensitive in the literature; we used portions of these promoters which were shown to be responsible for the mechanosensitivity. Click here and here for more in-depth notes on our literature review.
Promoter Cloning