"
Team:MIT k415032
From 2010.igem.org
(Difference between revisions)
(New page: {{CM_css}} <html> <head> <style> #topnav li.notebook a { background-color: #016b9d; } #topnav li.notebook ul { display: block; } #content { background-image: url('https://2010.igem.org...) |
|||
| Line 47: | Line 47: | ||
<tr><td> | <tr><td> | ||
<div class="bodybaby">K415031</div><br> | <div class="bodybaby">K415031</div><br> | ||
| - | From the <a href="https://2010.igem.org/Team:MIT"><b>2010 MIT iGEM Team</b></a>.<a style="float:right;" href="http://partsregistry.org/Part: | + | From the <a href="https://2010.igem.org/Team:MIT"><b>2010 MIT iGEM Team</b></a>.<a style="float:right;" href="http://partsregistry.org/Part:BBa_K415032"><b>See the Parts Registry Page!! →</b></a><br><br> |
<b class="bolded">Improved PLux/cI-OR Promoter</b><br>This part is a promoter that was designed as a correction to the anomalous <a href="http://partsregistry.org/Part:BBa_R0065">BBa_R0065</a> BioBrick. This part consists of a Lux Box binding site for the LuxR-C6HSL Complex; in addition, it contains two operator sites for the cI protein appropriated from the Lambda bacteriophage. Because basal expression without the LuxR-C6HSL complex present is expected to be minimal, and because the cI operator sites are positioned so as to block the critical -10 transcription initiation site on the promoter, if cI is high, the promoter is off; if AHL is low and cI is absent, the promoter is leaky; if AHL is high and cI is absent, the promoter is on.<br><br> | <b class="bolded">Improved PLux/cI-OR Promoter</b><br>This part is a promoter that was designed as a correction to the anomalous <a href="http://partsregistry.org/Part:BBa_R0065">BBa_R0065</a> BioBrick. This part consists of a Lux Box binding site for the LuxR-C6HSL Complex; in addition, it contains two operator sites for the cI protein appropriated from the Lambda bacteriophage. Because basal expression without the LuxR-C6HSL complex present is expected to be minimal, and because the cI operator sites are positioned so as to block the critical -10 transcription initiation site on the promoter, if cI is high, the promoter is off; if AHL is low and cI is absent, the promoter is leaky; if AHL is high and cI is absent, the promoter is on.<br><br> | ||
Revision as of 02:41, 27 October 2010
iGEM 2010
|
K415031 From the 2010 MIT iGEM Team.See the Parts Registry Page!! → Improved PLux/cI-OR Promoter This part is a promoter that was designed as a correction to the anomalous BBa_R0065 BioBrick. This part consists of a Lux Box binding site for the LuxR-C6HSL Complex; in addition, it contains two operator sites for the cI protein appropriated from the Lambda bacteriophage. Because basal expression without the LuxR-C6HSL complex present is expected to be minimal, and because the cI operator sites are positioned so as to block the critical -10 transcription initiation site on the promoter, if cI is high, the promoter is off; if AHL is low and cI is absent, the promoter is leaky; if AHL is high and cI is absent, the promoter is on. Slightly weaker binding than K415031.  ← K415031All Original Parts → |
