Team:MIT k415031

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From the <a href="http://2010.igem.org/Team:MIT"><b>2010 MIT iGEM Team</b></a>.<a style="float:right;" href="http://partsregistry.org/Part:BBa_K415031"><b>See the Parts Registry Page!! &rarr;</b></a><br><br>
From the <a href="http://2010.igem.org/Team:MIT"><b>2010 MIT iGEM Team</b></a>.<a style="float:right;" href="http://partsregistry.org/Part:BBa_K415031"><b>See the Parts Registry Page!! &rarr;</b></a><br><br>
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This part is a promoter that was designed as a correction to the anomalous <a href="http://partsregistry.org/Part:BBa_R0065">BBa_R0065</a> BioBrick. This part consists of a Lux Box binding site for the LuxR-C6HSL Complex; in addition, it contains two operator sites for the cI protein appropriated from the Lambda bacteriophage. Because basal expression without the LuxR-C6HSL complex present is expected to be minimal, and because the cI operator sites are positioned so as to block the critical -10 transcription initiation site on the promoter, if cI is high, the promoter is off; if AHL is low and cI is absent, the promoter is leaky; if AHL is high and cI is absent, the promoter is on. <br><br>
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<b class="bolded">Improved PLux/cI-OR Promoter</b>This part is a promoter that was designed as a correction to the anomalous <a href="http://partsregistry.org/Part:BBa_R0065">BBa_R0065</a> BioBrick. This part consists of a Lux Box binding site for the LuxR-C6HSL Complex; in addition, it contains two operator sites for the cI protein appropriated from the Lambda bacteriophage. Because basal expression without the LuxR-C6HSL complex present is expected to be minimal, and because the cI operator sites are positioned so as to block the critical -10 transcription initiation site on the promoter, if cI is high, the promoter is off; if AHL is low and cI is absent, the promoter is leaky; if AHL is high and cI is absent, the promoter is on. <br><br>
<a href="http://2010.igem.org/wiki/images/5/5a/Regulatory_yo.png" class="thickbox"><img src="http://2010.igem.org/wiki/images/5/5a/Regulatory_yo.png"></a><br><br>
<a href="http://2010.igem.org/wiki/images/5/5a/Regulatory_yo.png" class="thickbox"><img src="http://2010.igem.org/wiki/images/5/5a/Regulatory_yo.png"></a><br><br>

Revision as of 02:13, 27 October 2010

K415031

From the 2010 MIT iGEM Team.See the Parts Registry Page!! →

Improved PLux/cI-OR PromoterThis part is a promoter that was designed as a correction to the anomalous BBa_R0065 BioBrick. This part consists of a Lux Box binding site for the LuxR-C6HSL Complex; in addition, it contains two operator sites for the cI protein appropriated from the Lambda bacteriophage. Because basal expression without the LuxR-C6HSL complex present is expected to be minimal, and because the cI operator sites are positioned so as to block the critical -10 transcription initiation site on the promoter, if cI is high, the promoter is off; if AHL is low and cI is absent, the promoter is leaky; if AHL is high and cI is absent, the promoter is on.



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