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Team:MIT gateway
From 2010.igem.org
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<b class="bolded" id="miniprep">Plate Bacteria</b><br> | <b class="bolded" id="miniprep">Plate Bacteria</b><br> | ||
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Revision as of 21:36, 18 October 2010
iGEM 2010
bacterial biobrick construction |
The Mammalian team used Gateway cloning to assemble its composite parts.
1 General Gateway Protocol 2 Commented Protocol 2.1 Design PCR-Primers 2.2 Gel Purify PCR Product 2.3 Confirm PCR Product 2.4 Measure DNA 2.5 Calculate PCR Product Needed 2.6 Calculate volume of DONRTM needed 2.7 Prepare Gateway Reaction 2.8 Retrieve 5x BP Clonase II 2.9 Add BP-Clonase to Gateway Reaction 2.10 Incubate 2.11 Add ProteinaseK 2.12 Transform Bacteria 2.13 Plate Bacteria 3 Known Issues 4 Citation |
General Gateway Protocol Commented Protocol Design PCR-Primers Gel Purify PCR Product Confirm PCR Product Measure DNA Calculate PCR Product Needed Calculate volume of DONTR(TM) needed Prepare Gateway Reaction Retrieve 5x BP-Clonase II Add BP-Clonase to Gateway Reaction Incubate Add ProteinaseK Transform Bacteria Plate Bacteria Known Issues Citation 1. Untergasser A. “Cloning – Gateway BP-Reaction II” Untergasser's Lab. Summer 2006. (include here the date when you accessed these page). |
