Team:METU Turkey/Results Discussion/CooA Expression and Purification

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3-CooA Expression and Purification



1-Overexpression optimization
A. Expression vector comparison (pKK versus pTriEx)
A.1. Expression in BL21 with pTriEx
When we compare the below results, the expression of pTriEx vector is higher than the expression of pTriEx vector

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A.2. Expression in JM109 and BL21 with pKK vector
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B. Expression host comparison (JM109 versus BL21)



C. IPTG induction studies


D. Ferric Citrate effect


E. Soluble versus insoluble fraction


2-Purification optimization


A. Ion exchange chromatography with Sepharose Q (QS)





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