Team:METU Turkey/Results Discussion/CooA Expression and Purification

From 2010.igem.org

Revision as of 19:12, 27 October 2010 by Fundaguzey (Talk | contribs)

Home

 

3-CooA Expression and Purification



1-Overexpression optimization
A. Expression vector comparison (pKK versus pTriEx)
A.1. Expression in BL21 with pTriEx

w7

A.2. Expression in JM109 and BL21 with pKK vector
w7



B. Expression host comparison (JM109 versus BL21)



C. IPTG induction studies


D. Ferric Citrate effect


E. Soluble versus insoluble fraction


2-Purification optimization


A. Ion exchange chromatography with Sepharose Q (QS)





</p>

</td> </tr> </table> </div>

Retrieved from "http://2010.igem.org/Team:METU_Turkey/Results_Discussion/CooA_Expression_and_Purification"