Team:METU Turkey/Results Discussion/CooA Expression and Purification

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<br> A.1. Expression in BL21 with pTriEx
<br> A.1. Expression in BL21 with pTriEx
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<br> A.2. Expression in JM109 and BL21 with pKK vector
<br> A.2. Expression in JM109 and BL21 with pKK vector
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<br>  <html><div><a href="https://2010.igem.org/Team:METU_Turkey/Results_Discussion/pKK_versus_pTriEx"><strong>Details...</strong></a></div></html>
<br>  <html><div><a href="https://2010.igem.org/Team:METU_Turkey/Results_Discussion/pKK_versus_pTriEx"><strong>Details...</strong></a></div></html>
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<br> B. Expression host comparison (JM109 versus BL21)
<br> B. Expression host comparison (JM109 versus BL21)

Revision as of 19:11, 27 October 2010

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3-CooA Expression and Purification



1-Overexpression optimization
A. Expression vector comparison (pKK versus pTriEx)
A.1. Expression in BL21 with pTriEx

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A.2. Expression in JM109 and BL21 with pKK vector

<img style="border: 0px solid ; width: 300px; height: 300px;" alt="w7" src="Br2.jpg "></a>


B. Expression host comparison (JM109 versus BL21)



C. IPTG induction studies


D. Ferric Citrate effect


E. Soluble versus insoluble fraction


2-Purification optimization


A. Ion exchange chromatography with Sepharose Q (QS)





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