Team:METU Turkey/Conclusions

From 2010.igem.org

(Difference between revisions)
 
(4 intermediate revisions not shown)
Line 369: Line 369:
</td>
</td>
<td>
<td>
-
<p style="font-size:110%; color:#576f91; font-family:georgia,serif;"><br>
+
 
-
<br>
+
'''<br>
<br>- Cell sensor constructs which were based on CooA under T7 promoter in pTriEx vector transformed into E.coli BL21 DE3 host have failed to function.
<br>- Cell sensor constructs which were based on CooA under T7 promoter in pTriEx vector transformed into E.coli BL21 DE3 host have failed to function.
<br>  
<br>  
Line 382: Line 382:
there is a significant increase in expression levels when CO activates CooA.
there is a significant increase in expression levels when CO activates CooA.
<br>  
<br>  
-
<br><h3>Human Practice</h3>
+
<br>Human Practice
<br>  
<br>  
<br>- We hope to provide a technical guideline for other future gas sensing cell sensor projects with our E-CO sensor with enhanced dynamic range project.
<br>- We hope to provide a technical guideline for other future gas sensing cell sensor projects with our E-CO sensor with enhanced dynamic range project.
<br>
<br>
 +
'''

Latest revision as of 03:26, 28 October 2010

Home

 

Conclusions

'''

- Cell sensor constructs which were based on CooA under T7 promoter in pTriEx vector transformed into E.coli BL21 DE3 host have failed to function.

- Alternative sensor construct with pCooM promoter/RFP signal and CooA under pLac promoter in pSB1C3 vector transformed into E.coli Top10 host worked successfully ! E.coli cells transformed with this construct produces very noticable RFP signal -even by naked eye- when exposed to carbon monoxide.

- 16 biobricks are constructed and submitted to the registry of standart parts. 12 of 16 parts are sent to IGEM HQ as DNA samples.

- CooA overexpression and purification procedures were optimized.

- CooA binds to response elements in the absence and presence of CO as indicated by EMSA studies and basal level GFP/RFP expression. On the other hand, there is a significant increase in expression levels when CO activates CooA.

Human Practice

- We hope to provide a technical guideline for other future gas sensing cell sensor projects with our E-CO sensor with enhanced dynamic range project.
'''