Team:Lethbridge/Notebook/Lab Work/September

From 2010.igem.org

(Difference between revisions)
(→September 23, 2010)
(→September 23, 2010)
Line 204: Line 204:
<b>Objective:</b> Characterized catechol by xylE enzyme<br>
<b>Objective:</b> Characterized catechol by xylE enzyme<br>
-
<b>Method:</b> Measured absorbance of catechol (275nm) and 2-hydroxymuconate semialdehyde (380nm).
+
<b>Method:</b> Measured absorbance of catechol (275nm) and 2-hydroxymuconate semialdehyde (380nm).<br>
-
 
+
*<b>Protocol:</b>
-
 
+
*1) Grow cells in M9 minimal medium
-
 
+
*2) Take 1/10 dilution of cells
-
<br>
+
*3) Introduce 1&micro;L of 0.05M catechol solution into the cell dilution. (Final concentration of 50&microM;).
 +
*4) Quench the reaction with 5A% w/v trichloroacetate at certain time points. (0,15sec, 30sec, 45sec, 60sec, 2min).
 +
*5) Spin down cells.
 +
*6) Measure absorbance of supernatant.

Revision as of 03:32, 24 September 2010



Feel free to look around our notebook!

Here you can check out the work we have done in the lab, click on a month to take a look!

September 23, 2010

( JV)

Objective: Characterized catechol by xylE enzyme

Method: Measured absorbance of catechol (275nm) and 2-hydroxymuconate semialdehyde (380nm).

  • Protocol:
  • 1) Grow cells in M9 minimal medium
  • 2) Take 1/10 dilution of cells
  • 3) Introduce 1µL of 0.05M catechol solution into the cell dilution. (Final concentration of 50&microM;).
  • 4) Quench the reaction with 5A% w/v trichloroacetate at certain time points. (0,15sec, 30sec, 45sec, 60sec, 2min).
  • 5) Spin down cells.
  • 6) Measure absorbance of supernatant.
Retrieved from "http://2010.igem.org/Team:Lethbridge/Notebook/Lab_Work/September"