Team:Lethbridge/Notebook/Lab Work/October

From 2010.igem.org

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=<font color="white">October=
=<font color="white">October=
==<font color="white">October 2, 2010==
==<font color="white">October 2, 2010==
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===ADS===
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===<font color="white">ADS===
<b>Objective:</b> Purify plasmid DNA from transformed Mr. GENE synthesized DNA
<b>Objective:</b> Purify plasmid DNA from transformed Mr. GENE synthesized DNA
*<partinfo>K331022</partinfo>
*<partinfo>K331022</partinfo>
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<b>Method:</b> Use Qiagen miniprep method with BioBasic EZ-10 Spin columns.<br>
<b>Method:</b> Use Qiagen miniprep method with BioBasic EZ-10 Spin columns.<br>
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<b>Objective:</b> Create 20 new biobricks using recently arrived synthesized DNA, as well as <partinfo>B0015, R0010, R0040</partinfo>
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<b>Objective:</b> Create 20 new biobricks using recently arrived synthesized DNA and terminator <partinfo>B0015</partinfo>, pLac <partinfo>R0010</partinfo>, and pTet <partinfo>R0040</partinfo> to create the following new biobricks:
 +
*1)R0010 + K331022 to obtain (<font color="red">NAME BIOBRICK!!!!</font>)
 +
*2)R0040 + K331022 to obtain (<font color="red">NAME BIOBRICK!!!!</font>)
 +
*3)K331022 + B0015 to obtain (<font color="red">NAME BIOBRICK!!!!</font>)
 +
*4)K331022 + K331024 to obtain (<font color="red">NAME BIOBRICK!!!!</font>)
 +
*5)R0010 + K331023 to obtain (<font color="red">NAME BIOBRICK!!!!</font>)
 +
*6)R0040 + K331023 to obtain (<font color="red">NAME BIOBRICK!!!!</font>)
 +
*7)K331023 + B0015 to obtain (<font color="red">NAME BIOBRICK!!!!</font>)
 +
*8)K331023 + K331025 to obtain (<font color="red">NAME BIOBRICK!!!!</font>)
 +
*9)R0010 + K331024 to obtain (<font color="red">NAME BIOBRICK!!!!</font>)
 +
*10)R0040 + K331024 to obtain (<font color="red">NAME BIOBRICK!!!!</font>)
 +
*11)K331024 + B0015 to obtain (<font color="red">NAME BIOBRICK!!!!</font>)
 +
*12)K331024 + K331022 to obtain (<font color="red">NAME BIOBRICK!!!!</font>)
 +
*13)R0010 + K331025 to obtain (<font color="red">NAME BIOBRICK!!!!</font>)
 +
*14)R0040 + K331025 to obtain (<font color="red">NAME BIOBRICK!!!!</font>)
 +
*15)K331025 + B0015 to obtain (<font color="red">NAME BIOBRICK!!!!</font>)
 +
*16)K331025 + K331023 to obtain (<font color="red">NAME BIOBRICK!!!!</font>)
 +
*17)K331022 to obtain (<font color="red">NAME BIOBRICK!!!!</font>)
 +
*18)K331023 to obtain (<font color="red">NAME BIOBRICK!!!!</font>)
 +
*19)K331024 to obtain (<font color="red">NAME BIOBRICK!!!!</font>)
 +
*20)K331025 to obtain (<font color="red">NAME BIOBRICK!!!!</font>)
 +
All parts will be inserted into pSB1C3.
 +
<b>Method:</b> Use BioBrick Assembly Method<br>
 +
<b>Results:</b> Obtained <font color="red">RESULTS!!!</font> positive colonies.
 +
 
 +
 
<br>
<br>

Revision as of 00:30, 8 October 2010



Feel free to look around our notebook!

Here you can check out the work we have done in the lab, click on a month to take a look!

October

October 2, 2010

ADS

Objective: Purify plasmid DNA from transformed Mr. GENE synthesized DNA

  • <partinfo>K331022</partinfo>
  • <partinfo>K331023</partinfo>
  • <partinfo>K331024</partinfo>
  • <partinfo>K331025</partinfo>

Method: Use Qiagen miniprep method with BioBasic EZ-10 Spin columns.

Objective: Create 20 new biobricks using recently arrived synthesized DNA and terminator <partinfo>B0015</partinfo>, pLac <partinfo>R0010</partinfo>, and pTet <partinfo>R0040</partinfo> to create the following new biobricks:

  • 1)R0010 + K331022 to obtain (NAME BIOBRICK!!!!)
  • 2)R0040 + K331022 to obtain (NAME BIOBRICK!!!!)
  • 3)K331022 + B0015 to obtain (NAME BIOBRICK!!!!)
  • 4)K331022 + K331024 to obtain (NAME BIOBRICK!!!!)
  • 5)R0010 + K331023 to obtain (NAME BIOBRICK!!!!)
  • 6)R0040 + K331023 to obtain (NAME BIOBRICK!!!!)
  • 7)K331023 + B0015 to obtain (NAME BIOBRICK!!!!)
  • 8)K331023 + K331025 to obtain (NAME BIOBRICK!!!!)
  • 9)R0010 + K331024 to obtain (NAME BIOBRICK!!!!)
  • 10)R0040 + K331024 to obtain (NAME BIOBRICK!!!!)
  • 11)K331024 + B0015 to obtain (NAME BIOBRICK!!!!)
  • 12)K331024 + K331022 to obtain (NAME BIOBRICK!!!!)
  • 13)R0010 + K331025 to obtain (NAME BIOBRICK!!!!)
  • 14)R0040 + K331025 to obtain (NAME BIOBRICK!!!!)
  • 15)K331025 + B0015 to obtain (NAME BIOBRICK!!!!)
  • 16)K331025 + K331023 to obtain (NAME BIOBRICK!!!!)
  • 17)K331022 to obtain (NAME BIOBRICK!!!!)
  • 18)K331023 to obtain (NAME BIOBRICK!!!!)
  • 19)K331024 to obtain (NAME BIOBRICK!!!!)
  • 20)K331025 to obtain (NAME BIOBRICK!!!!)

All parts will be inserted into pSB1C3. Method: Use BioBrick Assembly Method
Results: Obtained RESULTS!!! positive colonies.




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