Team:Lethbridge/Notebook/Lab Work/June

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Contents

June 2010

June 1/2010

JV quantified the amount of DNA in gels run to date using ImageJ software. Results to be posted in working plasmids box.


June 2/2010

Objective: Isolate plasmid DNA (BBa_J33204) from DH5α cells

June 3/2010

Objective: Ligate pLacI to sRBS-Lum-dT using the three antibiotic assembly method (according to Tom Knight's protocol). Previous ligation had miniscule quantities of DNA in ligation, not surprising it didn't work.

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