Team:Lethbridge/Notebook/Lab Work/July
From 2010.igem.org
Liszabruder (Talk | contribs) |
Liszabruder (Talk | contribs) |
||
(24 intermediate revisions not shown) | |||
Line 57: | Line 57: | ||
</th> | </th> | ||
- | <th><a href="https://2010.igem.org/Team:Lethbridge/Notebook"> | + | <th><a href="https://2010.igem.org/Team:Lethbridge/Notebook/Lab_Work"> |
<img src="https://static.igem.org/mediawiki/2010/7/73/UofLNotebookbutton.jpg" width="80"/> | <img src="https://static.igem.org/mediawiki/2010/7/73/UofLNotebookbutton.jpg" width="80"/> | ||
</a> | </a> | ||
Line 94: | Line 94: | ||
</body> | </body> | ||
</html> | </html> | ||
+ | <hr> | ||
+ | |||
+ | <html> | ||
+ | <center> | ||
+ | <font color="white">Feel free to look around our notebook! | ||
+ | </center> | ||
+ | </html> | ||
+ | |||
+ | <html> | ||
+ | <body> | ||
+ | <center> | ||
+ | <table border="0" width="28%" style="background-color:#000000"> | ||
+ | |||
+ | <tr> | ||
+ | <th> | ||
+ | <div class="miniBar"> | ||
+ | <div class="countdown"><object type="application/x-shockwave-flash" data="http://www.oneplusyou.com/bb/files/countdown/countdown.swf?co=FFFFFF&bgcolor=000000&date_month=10&date_day=27&date_year=0&un=THE WIKI FREEZE&size=normal&mo=10&da=27&yr=2010" width="300" height="100"><param name="movie" value="http://www.oneplusyou.com/bb/files/countdown/countdown.swf?co=FFFFFF&bgcolor=000000&date_month=10&date_day=27&date_year=0&un=THE WIKI FREEZE&size=normal&mo=10&da=27&yr=2010" /><param name="bgcolor" value="#000000" /></object><img src="http://www.oneplusyou.com/q/img/bb_badges/countdown.jpg" alt="" style="display: none;" height="1" width="1" /></div> | ||
+ | <div class="miniContainer"> | ||
+ | |||
+ | <th><a href="https://2010.igem.org/Team:Lethbridge/Notebook/Lab_Work"> | ||
+ | <img src="https://static.igem.org/mediawiki/2010/7/73/UofLNotebookbutton.jpg" width="80"/> | ||
+ | </a> | ||
+ | </th> | ||
+ | |||
+ | <th><a href="https://2010.igem.org/Team:Lethbridge/Notebook/Protocols"> | ||
+ | <img src="https://static.igem.org/mediawiki/2010/9/91/UofLprotocolsbutton.jpg" width="60"/> | ||
+ | </a> | ||
+ | </th> | ||
+ | |||
+ | <th><a href="https://2010.igem.org/Team:Lethbridge/Notebook/Calendar"> | ||
+ | <img src="https://static.igem.org/mediawiki/2010/7/73/UofLcalendar.jpg" width="60"/> | ||
+ | </a> | ||
+ | </th> | ||
+ | |||
+ | <th> | ||
+ | <div class="miniBar"> | ||
+ | <div class="countdown"><object type="application/x-shockwave-flash" data="http://www.oneplusyou.com/bb/files/countdown/countdown.swf?co=FFFFFF&bgcolor=000000&date_month=11&date_day=05&date_year=0&un=THE IGEM JAMBOREE&size=normal&mo=11&da=05&yr=2010" width="300" height="100"><param name="movie" value="http://www.oneplusyou.com/bb/files/countdown/countdown.swf?co=FFFFFF&bgcolor=000000&date_month=11&date_day=05&date_year=0&un=THE IGEM JAMBOREE&size=normal&mo=11&da=05&yr=2010" /><param name="bgcolor" value="#000000" /></object><img src="http://www.oneplusyou.com/q/img/bb_badges/countdown.jpg" alt="" style="display: none;" height="1" width="1" /></div> | ||
+ | <div class="miniContainer"> | ||
+ | </th> | ||
+ | <tr> | ||
+ | </table> | ||
+ | </center> | ||
+ | </body> | ||
+ | </html> | ||
+ | |||
+ | <hr> | ||
+ | |||
+ | <html> | ||
+ | <center> | ||
+ | <font color="white">Here you can check out the work we have done in the lab, click on a month to take a look! | ||
+ | </center> | ||
+ | </html> | ||
+ | |||
+ | <html> | ||
+ | <body> | ||
+ | <center> | ||
+ | <table border="0" width="50%" style="background-color:#000000"> | ||
+ | |||
+ | <tr> | ||
+ | |||
+ | <th><a href="https://2010.igem.org/Team:Lethbridge/Notebook/Lab_Work/April"> | ||
+ | <img src="https://static.igem.org/mediawiki/2010/8/8a/UofLapril.jpg" width="60"/> | ||
+ | </a> | ||
+ | </th> | ||
+ | |||
+ | <th><a href="https://2010.igem.org/Team:Lethbridge/Notebook/Lab_Work/May"> | ||
+ | <img src="https://static.igem.org/mediawiki/2010/7/7b/UofLmaybutton.jpg" width="60"/> | ||
+ | </a> | ||
+ | </th> | ||
+ | |||
+ | <th><a href="https://2010.igem.org/Team:Lethbridge/Notebook/Lab_Work/June"> | ||
+ | <img src="https://static.igem.org/mediawiki/2010/8/80/UofLjunebutton.jpg" width="60"/> | ||
+ | </a> | ||
+ | </th> | ||
+ | |||
+ | <th><a href="https://2010.igem.org/Team:Lethbridge/Notebook/Lab_Work/July"> | ||
+ | <img src="https://static.igem.org/mediawiki/2010/5/53/UofLjulybutton.jpg" width="80"/> | ||
+ | </a> | ||
+ | </th> | ||
+ | |||
+ | <th><a href="https://2010.igem.org/Team:Lethbridge/Notebook/Lab_Work/August"> | ||
+ | <img src="https://static.igem.org/mediawiki/2010/1/15/UofLaugustbutton.jpg" width="60"/> | ||
+ | </a> | ||
+ | </th> | ||
+ | |||
+ | <th><a href="https://2010.igem.org/Team:Lethbridge/Notebook/Lab_Work/September"> | ||
+ | <img src="https://static.igem.org/mediawiki/2010/4/4d/UofLseptemberbutton.jpg" width="60"/> | ||
+ | </a> | ||
+ | </th> | ||
+ | |||
+ | <th><a href="https://2010.igem.org/Team:Lethbridge/Notebook/Lab_Work/October"> | ||
+ | <img src="https://static.igem.org/mediawiki/2010/4/4e/UofLoctoberbutton.jpg" width="60"/> | ||
+ | </a> | ||
+ | </th> | ||
+ | |||
+ | |||
+ | |||
+ | <tr> | ||
+ | </table> | ||
+ | </center> | ||
+ | </body> | ||
+ | </html> | ||
+ | <hr> | ||
+ | |||
+ | <BLOCKQUOTE> | ||
- | |||
- | |||
=<font color="white">July 2010= | =<font color="white">July 2010= | ||
==<font color="white">July 3/2010== | ==<font color="white">July 3/2010== | ||
Line 153: | Line 256: | ||
Ran gel at 100V for 45 minutes.<br> | Ran gel at 100V for 45 minutes.<br> | ||
- | < | + | <b>Results:</b> |
+ | [[image:Lethbridge_100705AVPCR.JPG|200px]]<br> | ||
==<font color="white">July 5/2010 Evening== | ==<font color="white">July 5/2010 Evening== | ||
Line 220: | Line 324: | ||
Ran at 100V for 80 mins.<br> | Ran at 100V for 80 mins.<br> | ||
- | < | + | <b>Results:</b> |
+ | [[image:Lethbridge_100705AV BW (2).jpg|200px]]<br> | ||
<font color ="Red">More to fill in, but Anthony does not understand the stuff written in the lab book</font><br> | <font color ="Red">More to fill in, but Anthony does not understand the stuff written in the lab book</font><br> | ||
Line 290: | Line 395: | ||
Ran at 100V for 40 minutes. Strained in EtBr for 10 minutes.<br> | Ran at 100V for 40 minutes. Strained in EtBr for 10 minutes.<br> | ||
- | < | + | <b>Results:</b> |
+ | [[image:Lethbridge_100710JV BW.JPG|200px]] | ||
==<font color="white">July 12/2010== | ==<font color="white">July 12/2010== | ||
Line 334: | Line 440: | ||
</table><br> | </table><br> | ||
Ran at 100V for 43 minutes. Stained in EtBr for 10 minutes.<br> | Ran at 100V for 43 minutes. Stained in EtBr for 10 minutes.<br> | ||
- | < | + | <b>Results:</b> |
+ | [[image:Lethbridge_100712HB REstriction.JPG|200px]]<br> | ||
4) Ligate restricted dT to the ends of the "Part 1" Biobricks.<br> | 4) Ligate restricted dT to the ends of the "Part 1" Biobricks.<br> | ||
Line 411: | Line 518: | ||
Ran at 100V for __ minutes. Stained in EtBr for 10 minutes.<br> | Ran at 100V for __ minutes. Stained in EtBr for 10 minutes.<br> | ||
- | < | + | <b>Results:</b> |
+ | [[image:Lethbridge_100713JV PCR.JPG|200px]]<br> | ||
==<font color="white">July 14/2010== | ==<font color="white">July 14/2010== | ||
Line 608: | Line 716: | ||
Gel ran for 70 minutes at 100V and was stained in EtBr for 10 minutes | Gel ran for 70 minutes at 100V and was stained in EtBr for 10 minutes | ||
- | < | + | <b>Results:</b> |
+ | [[image:Lethbridge_100719HBAVDigest.JPG|200px]]<br> | ||
Results after quantifying restriction digest | Results after quantifying restriction digest | ||
Line 650: | Line 759: | ||
*Ran a 1% Agarose gel to visualize PCR products. | *Ran a 1% Agarose gel to visualize PCR products. | ||
- | <font color =" | + | <b><font color="white">Results:</font></b> |
+ | Agarose gel did not show any bands.<br> | ||
==<font color="white">July 20, 2010 Evening== | ==<font color="white">July 20, 2010 Evening== | ||
Line 709: | Line 819: | ||
*TetR maxiprep<br> | *TetR maxiprep<br> | ||
Gel ran at 100V for 60minutes.<br> | Gel ran at 100V for 60minutes.<br> | ||
- | <b>Results:</b> The PCR's did not work. | + | <b>Results:</b> The PCR's did not work. However, the maxipreps show DNA<br> |
+ | [[image:Lethbridge_100722 JV BW.jpg|200px]] | ||
==<font color="white">July 23, 2010== | ==<font color="white">July 23, 2010== | ||
(JV)<br> | (JV)<br> | ||
<b>Objective:</b> Insert xylE, Mms6, and lumazine synthase into pET-28(a).<br> | <b>Objective:</b> Insert xylE, Mms6, and lumazine synthase into pET-28(a).<br> | ||
- | <b>Method:</b> A restriction digest was performed on xylE and Mms6 and ran for 90 minutes at 37 C | + | <b>Method:</b> A restriction digest was performed on xylE and Mms6 and ran for 90 minutes at 37 C<br> |
+ | <b>Results:</b>Did not see any cut out biobricks.<br> | ||
+ | [[image:Lethbridge_100723MaxiprepRestrictions.jpg|200px]] | ||
==<font color="white">July 27, 2010== | ==<font color="white">July 27, 2010== | ||
Line 746: | Line 859: | ||
<b>Method:</b> PCR amplified pSB1C3 received from iGEM headquarters. | <b>Method:</b> PCR amplified pSB1C3 received from iGEM headquarters. | ||
+ | |||
+ | |||
==<font color="white">July 29, 2010== | ==<font color="white">July 29, 2010== | ||
Line 755: | Line 870: | ||
<b>Results:</b> PCR amplification was successful and 0.2 µL of polymerase will be used per PCR reaction in the future.<br> | <b>Results:</b> PCR amplification was successful and 0.2 µL of polymerase will be used per PCR reaction in the future.<br> | ||
+ | |||
<br><br> | <br><br> | ||
<b>Objective:</b> To maxiprep EYFP (E0030), ECFP (E0020), and Lumazine Synthase (K249002).<br> | <b>Objective:</b> To maxiprep EYFP (E0030), ECFP (E0020), and Lumazine Synthase (K249002).<br> | ||
Line 771: | Line 887: | ||
<b>Objective:</b> Run PCR products from the morning on a 2% agarose gel.<br> | <b>Objective:</b> Run PCR products from the morning on a 2% agarose gel.<br> | ||
- | <b>Method:</b> Ran 2% agarose gel of the 11 minipreps and dT control and stained in EtBr for 17 minutes. | + | <b>Method:</b> Ran 2% agarose gel of the 11 minipreps and dT control and stained in EtBr for 17 minutes.<br> |
- | + | <b>Results:</b>PCR amplification of last year's parts not consistent with sizes listed on registry.<br> | |
- | <b>Results:</b> < | + | [[image:Lethbridge_100729 Registry Parts PCR KG.jpg|200px]]<br><br> |
+ | <br> |