Team:Lethbridge
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<b><font size=+2>May 10/2010</font>(in the lab:JV, KG, AV)</b><br> | <b><font size=+2>May 10/2010</font>(in the lab:JV, KG, AV)</b><br> | ||
<b>Objective:</b>Make 24 LB agar plates with 100µg/mL ampicillin antibiotic.<br> | <b>Objective:</b>Make 24 LB agar plates with 100µg/mL ampicillin antibiotic.<br> | ||
- | <b>Method:</b> | + | <b>Method:</b>Make 2L of LB media with agar<br> |
+ | 2x10g Tryptone<br> | ||
+ | 2X2.5g Yeast Extract<br> | ||
+ | 2x5g NaCl<br> | ||
+ | 2x10g Agar<br><br> | ||
+ | Continued <b> May 11/2010<br></b> | ||
+ | (Stock Ampicillin solution is 100mg/mL)<br> | ||
+ | Have 4x500mL of LB with Agar<br> | ||
+ | Add 500µL of stock ampicillin to 500mL of media<br><br> | ||
+ | <b><font size=+2>May 11/2010 Evening </font> (in the lab: KG, AV, MC, TF, JV, JS)<br></b> | ||
+ | <b>Objective:</b> To transform the following plasmids into DH5α <i>E.coli</i> cells. | ||
+ | <table><table border="3"> | ||
+ | <tr><td><b>Construct Name (2009)</td></b><td><b>Construct Location (2009)</b></td></tr> | ||
+ | <tr><td>Lumazine</td><td>J4</td></tr> | ||
+ | <tr><td>Lumazine-dT</td><td>J5,J6</td></tr> | ||
+ | <tr><td>sRBS-Lumazine-dT</td><td>J7,J8</td></tr> | ||
+ | <tr><td>pBAD-TetR</td><td>I4</td></tr> | ||
+ | <tr><td>pBAD</td><td>A5,F10</td></tr> | ||
+ | <tr><td>sRBS</td><td>D5,E10</td></tr> | ||
+ | <tr><td>pSB-CEYFP</td><td>E5,D6</td></tr> | ||
+ | <tr><td>pSB-NEYFP</td><td>F5,C6</td></tr> | ||
+ | <tr><td>C-term Tag</td><td>C10</td></tr> | ||
+ | <tr><td>N-term Tag</td><td>D9,D10</td></tr> | ||
+ | <tr><td>pTet</td><td>E4</td></tr> | ||
+ | <tr><td>EYFP</td><td>A4</td></tr> | ||
+ | <tr><td>CFP Complete</td><td>D4</td></tr> | ||
+ | </table> | ||
Revision as of 01:34, 15 May 2010
University of Lethbridge IGEM team
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April 13/2010 (In the Lab: JV, AS)
Objective: Test Restriction Endonucleases for Activity
Relevant Information:
Endonucleases available
April
May
April 13/2010 (In the Lab: JV, AS)
Objective: Test Restriction Endonucleases for Activity
Relevant Information:
Endonucleases available