Team:Lethbridge
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<a name="april"></a></font> | <a name="april"></a></font> | ||
<b><font size=+2>April 13/2010</font> (In the Lab: JV, AS)</b><br> | <b><font size=+2>April 13/2010</font> (In the Lab: JV, AS)</b><br> | ||
- | < | + | <b>Objective:</b> Test Restriction Endonucleases for Activity<br> |
- | < | + | <b>Relevant Information:</b><br> |
Endonucleases available | Endonucleases available | ||
<table><table border="3"> | <table><table border="3"> | ||
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<a name="may"></a> | <a name="may"></a> | ||
<b><font size=+2>May 5/2010</font>(in the lab: JV)</b><br> | <b><font size=+2>May 5/2010</font>(in the lab: JV)</b><br> | ||
- | < | + | <b>Objective:</b> Test Restriction Endonucleases for activity (take 2)<br> |
- | < | + | <b>Relevant Information:</b><br> |
Plasmid DNA used here will be "ES-pSB-CEYFP" from last year's plasmid stocks<br> | Plasmid DNA used here will be "ES-pSB-CEYFP" from last year's plasmid stocks<br> | ||
Prefix Enzymes are: EcoRI and XbaI<br> | Prefix Enzymes are: EcoRI and XbaI<br> | ||
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<a href="https://static.igem.org/mediawiki/2010/thumb/d/dc/100505JV-EnzymeTest1.JPG/800px-100505JV-EnzymeTest1.JPG"><img src="https://static.igem.org/mediawiki/2010/thumb/d/dc/100505JV-EnzymeTest1.JPG/800px-100505JV-EnzymeTest1.JPG" width="225" height="151"/></a><br> | <a href="https://static.igem.org/mediawiki/2010/thumb/d/dc/100505JV-EnzymeTest1.JPG/800px-100505JV-EnzymeTest1.JPG"><img src="https://static.igem.org/mediawiki/2010/thumb/d/dc/100505JV-EnzymeTest1.JPG/800px-100505JV-EnzymeTest1.JPG" width="225" height="151"/></a><br> | ||
This gel shows that SpeI does not cut on its own, and does not cut when combined with other enzymes<br> | This gel shows that SpeI does not cut on its own, and does not cut when combined with other enzymes<br> | ||
- | <b>Conclusion:</b> Test other source of SpeI to see if it has any activity.<br> | + | <b>Conclusion:</b> Test other source of SpeI to see if it has any activity.<br><br> |
+ | |||
+ | <b><font size=+2>May 6/2010</font>(in the lab:KG, AS)</b><br> | ||
+ | <b>Objective:</b> To check if the old SpeI enzyme (exp date: March 2011) will cleave plasmid DNA, since we believe the newer SpeI enzyme (exp date: 2012) does not. | ||
Revision as of 05:19, 14 May 2010
University of Lethbridge IGEM team
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April
May
April 13/2010 (In the Lab: JV, AS)
Objective: Test Restriction Endonucleases for Activity
Relevant Information:
Endonucleases available
April
May
April 13/2010 (In the Lab: JV, AS)
Objective: Test Restriction Endonucleases for Activity
Relevant Information:
Endonucleases available