Team:Lethbridge
From 2010.igem.org
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<tr><td><b>Total</b></td><td>19.75</td><td>69</td></tr> | <tr><td><b>Total</b></td><td>19.75</td><td>69</td></tr> | ||
</table><br> | </table><br> | ||
+ | To each tube, add <b>19.75µL</b> of master mix and <b>0.25µL</b> of enzyme<br> | ||
+ | Incubated reaction mixes at 37<sup>o</sup>C (Start:7:00pm; End:7:45pm)<br> | ||
+ | Add 3.3µL of 6x loading dye to each reaction mixture and load 10µL final volume onto a 1% agarose (in TAE) gel.<br> | ||
+ | Add 1µL of 6x loading dye to 1µL of GeneRuler 1kb ladder (at 0.5µg/µL)<br> | ||
+ | Gel loading order as follows:<br> | ||
+ | <table><table border="3"> | ||
+ | <tr><td><b>Lane</b></td><td><b>Sample</b></td></tr> | ||
+ | <tr><td>1</td><td>1kb Ladder</td></tr> | ||
+ | <tr><td>2</td><td>Tango Control</td></tr> | ||
+ | <tr><td>3</td><td>DpnI (Tango)</td></tr> | ||
+ | <tr><td>4</td><td>BcuI/SpeI (Tango)</td></tr> | ||
+ | <tr><td>5</td><td>XbaI (Tango)</td></tr> | ||
+ | <tr><td>6</td><td>EcoRI (Red)</td></tr> | ||
+ | <tr><td>7</td><td>PstI (Red)</td></tr> | ||
+ | <tr><td>8</td><td>Red Control</td></tr> | ||
+ | <tr><td>9</td><td>Empty</td></tr> | ||
+ | <tr><td>10</td><td>Empty</td></tr> | ||
</table> | </table> | ||
Revision as of 03:41, 14 May 2010
University of Lethbridge IGEM team
- Main
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Our team is based yada yada
- Team Pics
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Content 3
Pics of us
Overview
- Project 1
- Porejct 2
Content 1
Content 2
April 13/2010 (In the Lab: JV, AS)
Objective: Test Restriction Endonucleases for Activity
Related Information:
Endonucleases available
Objective: Test Restriction Endonucleases for Activity
Related Information:
Endonucleases available