Team:LMU-Munich/TEV-System/Schedule/Aim 1

From 2010.igem.org

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== Lab-Notebook ==
== Lab-Notebook ==
-
This notebook should structure our work and progress
+
This notebook is used to structure our work and progress
== DNA-multiplication ==
== DNA-multiplication ==
-
necessary
+
It is necessary to increase the amount of DNA we have got in order to work properly with it.
-
=== BBJ52034 ===
+
=== CMV-Promoter from BBJ52034 ===
 +
====Work====
 +
We have to proliferate E. colis containing this plasmid and then extract the plasmid from the bacteria.
 +
====Progress====
-
Proliferate E. colis containing this plasmid and extract the plasmid
+
8/9+10 Proliferation done by Corinna, Laura, Alex, Julia
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10.8. Proliferation done by Corinna, Laura, Alex, Julia
+
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=== eGFP ===
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8/11 Planned extraction by Jara, Emanuel
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...
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=== eGFP ===
 +
This Biobrick is needed several times.
 +
====Work====
 +
It has to be ...(?)
 +
====Progress====
==PCR-steps==
==PCR-steps==

Revision as of 20:03, 10 August 2010


Contents

Lab-Notebook

This notebook is used to structure our work and progress

DNA-multiplication

It is necessary to increase the amount of DNA we have got in order to work properly with it.

CMV-Promoter from BBJ52034

Work

We have to proliferate E. colis containing this plasmid and then extract the plasmid from the bacteria.

Progress

8/9+10 Proliferation done by Corinna, Laura, Alex, Julia

8/11 Planned extraction by Jara, Emanuel

eGFP

This Biobrick is needed several times.

Work

It has to be ...(?)

Progress

PCR-steps

..

mutagenesis

...

fusion

...

cloning

fusions

insert in eukaryotic genom

if there is time left