PCR and mutagenisis of the digestion sites
PCR1a: replication of Pdu D with mutagenisis
File:PCR1aP
PCR1b: replication of Pdu D with mutagenisis
File:PCR1bP
PCR2: Joining PCR of PduD
File:PCR2P
PCR3a: replication of Pdu C with mutagenisis
File:PCR3aP
PCR3b: replication of Pdu C with mutagenisis
File:PCR3bP
PCR4: Joining PCR of Pdu C
File:PCR4P
PCR5: replication of AurF to be joined with Pdu D
File:PCR5P
PCR6a: replication of LctO with mutagenisis
File:PCR6aP
PCR6b: replication of LctO with mutagenisis
File:PCR6bP
PCR7: Joining PCR of LctO
File:PCR7P
PCR8a: replication of LctO with mutagenisis
File:PCR8aP
PCR8b: replication of LctO with mutagenisis
File:PCR8bP
PCR9: Joining PCR of LctO
File:PCR9P
PCR10: Joining PCR of AurF and PduD
File:PCR10P
PCR11: Joining PCR of LctO and PduC
File:PCR11P
PCR12a: replication of the Pdu-Operon with mutagenisis
File:PCR12aP
PCR12b: replication of the Pdu-Operon with mutagenisis
File:PCR12bP
PCR13: Joining PCR of the Pdu-Operon
File:PCR131517P
PCR14a: replication of the Pdu-Operon with mutagenisis
File:PCR14aP
PCR14b: replication of the Pdu-Operon with mutagenisis
File:PCR14bP
PCR15: Joining PCR of the Pdu-Operon
File:PCR131517P
PCR16a: replication of the Pdu-Operon with mutagenisis
File:PCR16aP
PCR16b: replication of the Pdu-Operon with mutagenisis
File:PCR16bP
PCR17: Joining PCR of the Pdu-Operon
File:PCR131517P
In Vitro testing of the chemical kinetics with the enzymes
Transforming the construct in bacteria
Testing the system
|
"
