Team:LMU-Munich/Schedule

From 2010.igem.org

(Difference between revisions)
(→PCR and mutagenisis of the digestion sites)
(→PCR and mutagenisis of the digestion sites)
Line 11: Line 11:
[[Image:PCR1bP.jpg]]
[[Image:PCR1bP.jpg]]
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Primer used:[[Team:LMU-Munich/Schedule/mpduD_fwd| mpduD fwd][[Team:LMU-Munich/Schedule/3P2D| 3P2D]] expected product size:  bp
+
Primer used:[[Team:LMU-Munich/Schedule/mpduD_fwd| mpduD fwd]][[Team:LMU-Munich/Schedule/3P2D| 3P2D]] expected product size:  bp
Line 17: Line 17:
[[Image:PCR2P.jpg]]
[[Image:PCR2P.jpg]]
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Primer used:[[Team:LMU-Munich/Schedule/1P1D| 1P1D][[Team:LMU-Munich/Schedule/3P2D| 3P2D]] expected product size:  bp
+
Primer used:[[Team:LMU-Munich/Schedule/1P1D| 1P1D]][[Team:LMU-Munich/Schedule/3P2D| 3P2D]] expected product size:  bp
Line 23: Line 23:
[[Image:PCR3aP.jpg]]
[[Image:PCR3aP.jpg]]
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Primer used:[[Team:LMU-Munich/Schedule/2P1C| 2P1C][[Team:LMU-Munich/Schedule/mpduC_rev| mpduC rev]]expected product size:  bp
+
Primer used:[[Team:LMU-Munich/Schedule/2P1C| 2P1C]][[Team:LMU-Munich/Schedule/mpduC_rev| mpduC rev]]expected product size:  bp
Line 29: Line 29:
[[Image:PCR3bP.jpg]]
[[Image:PCR3bP.jpg]]
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Primer used:[[Team:LMU-Munich/Schedule/mpduC_fwd| mpduC fwd][[Team:LMU-Munich/Schedule/4P2C| 4P2C]] expected product size:  bp
+
Primer used:[[Team:LMU-Munich/Schedule/mpduC_fwd| mpduC fwd]][[Team:LMU-Munich/Schedule/4P2C| 4P2C]] expected product size:  bp
Line 35: Line 35:
[[Image:PCR4P.jpg]]
[[Image:PCR4P.jpg]]
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Primer used:[[Team:LMU-Munich/Schedule/2P1C| 2P1C][[Team:LMU-Munich/Schedule/4P2C| 4P2C]] expected product size:  bp
+
Primer used:[[Team:LMU-Munich/Schedule/2P1C| 2P1C]][[Team:LMU-Munich/Schedule/4P2C| 4P2C]] expected product size:  bp
Line 41: Line 41:
[[Image:PCR5P.jpg]]
[[Image:PCR5P.jpg]]
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Primer used:[[Team:LMU-Munich/Schedule/5P3AD| 5P3AD][[Team:LMU-Munich/Schedule/9P4A| 9P4A]] expected product size:  bp
+
Primer used:[[Team:LMU-Munich/Schedule/5P3AD| 5P3AD]][[Team:LMU-Munich/Schedule/9P4A| 9P4A]] expected product size:  bp
Line 47: Line 47:
[[Image:PCR6aP.jpg]]
[[Image:PCR6aP.jpg]]
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Primer used:[[Team:LMU-Munich/Schedule/8P3LC| 8P3LC][[Team:LMU-Munich/Schedule/m1lctO_rev| m1lctO rev]] expected product size:  bp
+
Primer used:[[Team:LMU-Munich/Schedule/8P3LC| 8P3LC]][[Team:LMU-Munich/Schedule/m1lctO_rev| m1lctO rev]] expected product size:  bp
Line 53: Line 53:
[[Image:PCR6bP.jpg]]
[[Image:PCR6bP.jpg]]
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Primer used:[[Team:LMU-Munich/Schedule/m1lctO_fwd| m1lctO fwd][[Team:LMU-Munich/Schedule/10P4L| 10P4L]] expected product size:  bp
+
Primer used:[[Team:LMU-Munich/Schedule/m1lctO_fwd| m1lctO fwd]][[Team:LMU-Munich/Schedule/10P4L| 10P4L]] expected product size:  bp
Line 59: Line 59:
[[Image: PCR7P.jpg]]
[[Image: PCR7P.jpg]]
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Primer used:[[Team:LMU-Munich/Schedule/8P3LC| 8P3LC][[Team:LMU-Munich/Schedule/10P4L| 10P4L]]expected product size:  bp
+
Primer used:[[Team:LMU-Munich/Schedule/8P3LC| 8P3LC]][[Team:LMU-Munich/Schedule/10P4L| 10P4L]]expected product size:  bp
Line 65: Line 65:
[[Image:PCR8aP.jpg]]
[[Image:PCR8aP.jpg]]
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Primer used:[[Team:LMU-Munich/Schedule/8P3LC| 8P3LC][[Team:LMU-Munich/Schedule/m2lctO_rev| m2lctO rev]] expected product size:  bp
+
Primer used:[[Team:LMU-Munich/Schedule/8P3LC| 8P3LC]][[Team:LMU-Munich/Schedule/m2lctO_rev| m2lctO rev]] expected product size:  bp
Line 71: Line 71:
[[Image:PCR8bP.jpg]]
[[Image:PCR8bP.jpg]]
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Primer used:[[Team:LMU-Munich/Schedule/m2lctO_fwd| m2lctO fwd][[Team:LMU-Munich/Schedule/10P4L| 10P4L]]expected product size:  bp
+
Primer used:[[Team:LMU-Munich/Schedule/m2lctO_fwd| m2lctO fwd]][[Team:LMU-Munich/Schedule/10P4L| 10P4L]]expected product size:  bp
Line 77: Line 77:
[[Image:PCR9P.jpg]]
[[Image:PCR9P.jpg]]
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Primer used:[[Team:LMU-Munich/Schedule/8P3LC| 8P3LC][[Team:LMU-Munich/Schedule/10P4L| 10P4L]]expected product size:  bp
+
Primer used:[[Team:LMU-Munich/Schedule/8P3LC| 8P3LC]][[Team:LMU-Munich/Schedule/10P4L| 10P4L]]expected product size:  bp
Line 83: Line 83:
[[Image:PCR10P.jpg]]
[[Image:PCR10P.jpg]]
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Primer used:[[Team:LMU-Munich/Schedule/1P1D| 1P1D][[Team:LMU-Munich/Schedule/9P4A| 9P4A]]expected product size:  bp
+
Primer used:[[Team:LMU-Munich/Schedule/1P1D| 1P1D]][[Team:LMU-Munich/Schedule/9P4A| 9P4A]]expected product size:  bp
Line 89: Line 89:
[[Image:PCR11P.jpg]]
[[Image:PCR11P.jpg]]
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Primer used:[[Team:LMU-Munich/Schedule/2P1C| 2P1C][[Team:LMU-Munich/Schedule/10P4L| 10P4L]] expected product size:  bp
+
Primer used:[[Team:LMU-Munich/Schedule/2P1C| 2P1C]][[Team:LMU-Munich/Schedule/10P4L| 10P4L]] expected product size:  bp
Line 95: Line 95:
[[Image:PCR12aP.jpg]]
[[Image:PCR12aP.jpg]]
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Primer used:[[Team:LMU-Munich/Schedule/pduA_fwd| pduA fwd][[Team:LMU-Munich/Schedule/mpduA_rev| mpduA rev]] expected product size:  bp
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Primer used:[[Team:LMU-Munich/Schedule/pduA_fwd| pduA fwd]][[Team:LMU-Munich/Schedule/mpduA_rev| mpduA rev]] expected product size:  bp
Line 102: Line 102:
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Primer used:[[Team:LMU-Munich/Schedule/mpduA_fwd| mpduA fwd][[Team:LMU-Munich/Schedule/pduU_rev|pduU rev]] expected product size:  bp
+
Primer used:[[Team:LMU-Munich/Schedule/mpduA_fwd| mpduA fwd]][[Team:LMU-Munich/Schedule/pduU_rev|pduU rev]] expected product size:  bp
Line 108: Line 108:
[[Image:PCR131517P.jpg]]
[[Image:PCR131517P.jpg]]
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Primer used:[[Team:LMU-Munich/Schedule/pduA_fwd| pduA fwd][[Team:LMU-Munich/Schedule/pduU_rev|pduU rev]]expected product size:  bp
+
Primer used:[[Team:LMU-Munich/Schedule/pduA_fwd| pduA fwd]][[Team:LMU-Munich/Schedule/pduU_rev|pduU rev]]expected product size:  bp
Line 114: Line 114:
[[Image:PCR14aP.jpg]]
[[Image:PCR14aP.jpg]]
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Primer used:[[Team:LMU-Munich/Schedule/pduA_fwd| pduA fwd][[Team:LMU-Munich/Schedule/mpduB_rev|mpduB rev]]expected product size:  bp
+
Primer used:[[Team:LMU-Munich/Schedule/pduA_fwd| pduA fwd]][[Team:LMU-Munich/Schedule/mpduB_rev|mpduB rev]]expected product size:  bp
Line 120: Line 120:
[[Image:PCR14bP.jpg]]
[[Image:PCR14bP.jpg]]
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Primer used:[[Team:LMU-Munich/Schedule/mpduB_fwd| mpduB fwd][[Team:LMU-Munich/Schedule/pduU_rev|pduU rev]]expected product size:  bp
+
Primer used:[[Team:LMU-Munich/Schedule/mpduB_fwd| mpduB fwd]][[Team:LMU-Munich/Schedule/pduU_rev|pduU rev]]expected product size:  bp
Line 132: Line 132:
[[Image:PCR16aP.jpg]]
[[Image:PCR16aP.jpg]]
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Primer used:[[Team:LMU-Munich/Schedule/pduA_fwd|pduA fwd][[Team:LMU-Munich/Schedule/mpduJ_rev|mpduJ rev]]expected product size:  bp
+
Primer used:[[Team:LMU-Munich/Schedule/pduA_fwd|pduA fwd]][[Team:LMU-Munich/Schedule/mpduJ_rev|mpduJ rev]]expected product size:  bp
Line 138: Line 138:
[[Image:PCR16bP.jpg]]
[[Image:PCR16bP.jpg]]
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Primer used:[[Team:LMU-Munich/Schedule/mpduJ_fwd|mpduJ fwd][[Team:LMU-Munich/Schedule/pduU_rev|pduU rev]]expected product size:  bp
+
Primer used:[[Team:LMU-Munich/Schedule/mpduJ_fwd|mpduJ fwd]][[Team:LMU-Munich/Schedule/pduU_rev|pduU rev]]expected product size:  bp
Line 144: Line 144:
[[Image:PCR131517P.jpg]]
[[Image:PCR131517P.jpg]]
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Primer used:[[Team:LMU-Munich/Schedule/pduA_fwd|pduA fwd][[Team:LMU-Munich/Schedule/pduU_rev|pduU rev]]expected product size:  bp
+
Primer used:[[Team:LMU-Munich/Schedule/pduA_fwd|pduA fwd]][[Team:LMU-Munich/Schedule/pduU_rev|pduU rev]]expected product size:  bp
==In Vitro testing of the chemical kinetics with the enzymes==
==In Vitro testing of the chemical kinetics with the enzymes==

Revision as of 12:58, 20 August 2010


Contents

PCR and mutagenisis of the digestion sites

PCR1a: replication of Pdu D with mutagenesis [ ] PCR1aP.jpg

Primer used: 1P1D mpduD_rev expected product size: bp


PCR1b: replication of Pdu D with mutagenesis [ ] PCR1bP.jpg

Primer used: mpduD fwd 3P2D expected product size: bp


PCR2: Joining PCR of PduD [ ] PCR2P.jpg

Primer used: 1P1D 3P2D expected product size: bp


PCR3a: replication of Pdu C with mutagenesis [ ] PCR3aP.jpg

Primer used: 2P1C mpduC revexpected product size: bp


PCR3b: replication of Pdu C with mutagenesis [ ] PCR3bP.jpg

Primer used: mpduC fwd 4P2C expected product size: bp


PCR4: Joining PCR of Pdu C [ ] PCR4P.jpg

Primer used: 2P1C 4P2C expected product size: bp


PCR5: replication of AurF to be joined with Pdu D [ ] PCR5P.jpg

Primer used: 5P3AD 9P4A expected product size: bp


PCR6a: replication of LctO with mutagenesis [ ] PCR6aP.jpg

Primer used: 8P3LC m1lctO rev expected product size: bp


PCR6b: replication of LctO with mutagenesis [ ] PCR6bP.jpg

Primer used: m1lctO fwd 10P4L expected product size: bp


PCR7: Joining PCR of LctO [ ] PCR7P.jpg

Primer used: 8P3LC 10P4Lexpected product size: bp


PCR8a: replication of LctO with mutagenesis [ ] PCR8aP.jpg

Primer used: 8P3LC m2lctO rev expected product size: bp


PCR8b: replication of LctO with mutagenesis [ ] PCR8bP.jpg

Primer used: m2lctO fwd 10P4Lexpected product size: bp


PCR9: Joining PCR of LctO [ ] PCR9P.jpg

Primer used: 8P3LC 10P4Lexpected product size: bp


PCR10: Joining PCR of AurF and PduD [ ] PCR10P.jpg

Primer used: 1P1D 9P4Aexpected product size: bp


PCR11: Joining PCR of LctO and PduC [ ] PCR11P.jpg

Primer used: 2P1C 10P4L expected product size: bp


PCR12a: replication of the Pdu-Operon with mutagenesis [ ] PCR12aP.jpg

Primer used: pduA fwd mpduA rev expected product size: bp


PCR12b: replication of the Pdu-Operon with mutagenesis [ ] PCR12bP.jpg


Primer used: mpduA fwdpduU rev expected product size: bp


PCR13: Joining PCR of the Pdu-Operon [ ] PCR131517P.jpg

Primer used: pduA fwdpduU revexpected product size: bp


PCR14a: replication of the Pdu-Operon with mutagenesis [ ] PCR14aP.jpg

Primer used: pduA fwdmpduB revexpected product size: bp


PCR14b: replication of the Pdu-Operon with mutagenesis [ ] PCR14bP.jpg

Primer used: mpduB fwdpduU revexpected product size: bp


PCR15: Joining PCR of the Pdu-Operon [ ] PCR131517P.jpg

Primer used:[[Team:LMU-Munich/Schedule/pduA_fwd|pduA fwd]pduU revexpected product size: bp


PCR16a: replication of the Pdu-Operon with mutagenesis [ ] PCR16aP.jpg

Primer used:pduA fwdmpduJ revexpected product size: bp


PCR16b: replication of the Pdu-Operon with mutagenesis [ ] PCR16bP.jpg

Primer used:mpduJ fwdpduU revexpected product size: bp


PCR17: Joining PCR of the Pdu-Operon [ ] PCR131517P.jpg

Primer used:pduA fwdpduU revexpected product size: bp

In Vitro testing of the chemical kinetics with the enzymes

Transforming the construct in bacteria

Testing the system
 

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