Team:LMU-Munich/Schedule

From 2010.igem.org

(Difference between revisions)

Revision as of 11:55, 20 August 2010


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PCR and mutagenisis of the digestion sites

PCR1a: replication of Pdu D with mutagenesis [ ]

expected product size: bp

PCR1b: replication of Pdu D with mutagenesis [ ]

expected product size: bp

PCR2: Joining PCR of PduD [ ]

expected product size: bp

PCR3a: replication of Pdu C with mutagenesis [ ]

expected product size: bp

PCR3b: replication of Pdu C with mutagenesis [ ]

expected product size: bp

PCR4: Joining PCR of Pdu C [ ]

expected product size: bp

PCR5: replication of AurF to be joined with Pdu D [ ]

expected product size: bp

PCR6a: replication of LctO with mutagenesis [ ]

expected product size: bp

PCR6b: replication of LctO with mutagenesis [ ]

expected product size: bp

PCR7: Joining PCR of LctO [ ]

expected product size: bp

PCR8a: replication of LctO with mutagenesis [ ] expected product size: bp

PCR8b: replication of LctO with mutagenesis [ ]

expected product size: bp

PCR9: Joining PCR of LctO [ ]

expected product size: bp

PCR10: Joining PCR of AurF and PduD [ ]

expected product size: bp

PCR11: Joining PCR of LctO and PduC [ ]

expected product size: bp

PCR12a: replication of the Pdu-Operon with mutagenesis [ ]

expected product size: bp

PCR12b: replication of the Pdu-Operon with mutagenesis [ ]

expected product size: bp

PCR13: Joining PCR of the Pdu-Operon [ ]

expected product size: bp

PCR14a: replication of the Pdu-Operon with mutagenesis [ ]

expected product size: bp

PCR14b: replication of the Pdu-Operon with mutagenesis [ ]

expected product size: bp

PCR15: Joining PCR of the Pdu-Operon [ ]

expected product size: bp

PCR16a: replication of the Pdu-Operon with mutagenesis [ ]

expected product size: bp

PCR16b: replication of the Pdu-Operon with mutagenesis [ ]

expected product size: bp

PCR17: Joining PCR of the Pdu-Operon [ ]

expected product size: bp

@@ Line 4: / Line 4: @@
 <b>PCR1a: replication of Pdu D with mutagenesis [ ] </b>
 [[Image:PCR1aP.jpg]]
 expected product size:  bp
@@ Line 9: / Line 10: @@
 <b>PCR1b: replication of Pdu D with mutagenesis [ ] </b>
 [[Image:PCR1bP.jpg]]
 expected product size:  bp
@@ Line 14: / Line 16: @@
 <b>PCR2: Joining PCR of PduD [ ] </b>
 [[Image:PCR2P.jpg]]
 expected product size:  bp
@@ Line 19: / Line 22: @@
 <b>PCR3a: replication of Pdu C with mutagenesis [ ] </b>
 [[Image:PCR3aP.jpg]]
 expected product size:  bp
@@ Line 24: / Line 28: @@
 <b>PCR3b: replication of Pdu C with mutagenesis [ ] </b>
 [[Image:PCR3bP.jpg]]
 expected product size:  bp
@@ Line 29: / Line 34: @@
 <b>PCR4: Joining PCR of Pdu C [ ] </b>
 [[Image:PCR4P.jpg]]
 expected product size:  bp
@@ Line 34: / Line 40: @@
 <b>PCR5: replication of AurF to be joined with Pdu D [ ] </b>
 [[Image:PCR5P.jpg]]
 expected product size:  bp
@@ Line 39: / Line 46: @@
 <b>PCR6a: replication of LctO with mutagenesis [ ] </b>
 [[Image:PCR6aP.jpg]]
 expected product size:  bp
@@ Line 44: / Line 52: @@
 <b>PCR6b: replication of LctO with mutagenesis [ ] </b>
 [[Image:PCR6bP.jpg]]
 expected product size:  bp
@@ Line 49: / Line 58: @@
 <b>PCR7: Joining PCR of LctO [ ] </b>
 [[Image: PCR7P.jpg]]
 expected product size:  bp
@@ Line 59: / Line 69: @@
 <b>PCR8b: replication of LctO with mutagenesis [ ] </b>
 [[Image:PCR8bP.jpg]]
 expected product size:  bp
@@ Line 64: / Line 75: @@
 <b>PCR9: Joining PCR of LctO [ ] </b>
 [[Image:PCR9P.jpg]]
 expected product size:  bp
@@ Line 69: / Line 81: @@
 <b>PCR10: Joining PCR of AurF and PduD [ ] </b>
 [[Image:PCR10P.jpg]]
 expected product size:  bp
@@ Line 74: / Line 87: @@
 <b>PCR11: Joining PCR of LctO and PduC [ ] </b>
 [[Image:PCR11P.jpg]]
 expected product size:  bp
@@ Line 79: / Line 93: @@
 <b>PCR12a: replication of the Pdu-Operon with mutagenesis [ ] </b>
 [[Image:PCR12aP.jpg]]
 expected product size:  bp
@@ Line 84: / Line 99: @@
 <b>PCR12b: replication of the Pdu-Operon with mutagenesis [ ] </b>
 [[Image:PCR12bP.jpg]]
 expected product size:  bp
@@ Line 89: / Line 105: @@
 <b>PCR13: Joining PCR of the Pdu-Operon [ ] </b>
 [[Image:PCR131517P.jpg]]
 expected product size:  bp
@@ Line 94: / Line 111: @@
 <b>PCR14a: replication of the Pdu-Operon with mutagenesis [ ] </b>
 [[Image:PCR14aP.jpg]]
 expected product size:  bp
@@ Line 99: / Line 117: @@
 <b>PCR14b: replication of the Pdu-Operon with mutagenesis [ ] </b>
 [[Image:PCR14bP.jpg]]
 expected product size:  bp
@@ Line 104: / Line 123: @@
 <b>PCR15: Joining PCR of the Pdu-Operon [ ] </b>
 [[Image:PCR131517P.jpg]]
 expected product size:  bp
@@ Line 109: / Line 129: @@
 <b>PCR16a: replication of the Pdu-Operon with mutagenesis [ ] </b>
 [[Image:PCR16aP.jpg]]
 expected product size:  bp
@@ Line 114: / Line 135: @@
 <b>PCR16b: replication of the Pdu-Operon with mutagenesis [ ] </b>
 [[Image:PCR16bP.jpg]]
 expected product size:  bp
-<b>PCR17: Joining PCR of the Pdu-Operon
+<b>PCR17: Joining PCR of the Pdu-Operon [ ] </b>
 [[Image:PCR131517P.jpg]]
 expected product size:  bp

Team:LMU-Munich/Schedule

From 2010.igem.org

Revision as of 11:55, 20 August 2010

Contents

PCR and mutagenisis of the digestion sites

In Vitro testing of the chemical kinetics with the enzymes

Transforming the construct in bacteria

Testing the system