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Team:LMU-Munich/Schedule
From 2010.igem.org
(→PCR and mutagenisis of the digestion sites) |
(→PCR and mutagenisis of the digestion sites) |
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PCR1a: replication of Pdu D with mutagenisis | PCR1a: replication of Pdu D with mutagenisis | ||
| - | [[Image:PCR1aP]] | + | [[Image:PCR1aP.jpg]] |
PCR1b: replication of Pdu D with mutagenisis | PCR1b: replication of Pdu D with mutagenisis | ||
| - | [[Image:PCR1bP]] | + | [[Image:PCR1bP.jpg]] |
PCR2: Joining PCR of PduD | PCR2: Joining PCR of PduD | ||
| - | [[Image:PCR2P]] | + | [[Image:PCR2P.jpg]] |
PCR3a: replication of Pdu C with mutagenisis | PCR3a: replication of Pdu C with mutagenisis | ||
| - | [[Image:PCR3aP]] | + | [[Image:PCR3aP.jpg]] |
PCR3b: replication of Pdu C with mutagenisis | PCR3b: replication of Pdu C with mutagenisis | ||
| - | [[Image:PCR3bP]] | + | [[Image:PCR3bP.jpg]] |
PCR4: Joining PCR of Pdu C | PCR4: Joining PCR of Pdu C | ||
| - | [[Image:PCR4P]] | + | [[Image:PCR4P.jpg]] |
PCR5: replication of AurF to be joined with Pdu D | PCR5: replication of AurF to be joined with Pdu D | ||
| - | [[Image:PCR5P]] | + | [[Image:PCR5P.jpg]] |
PCR6a: replication of LctO with mutagenisis | PCR6a: replication of LctO with mutagenisis | ||
| - | [[Image:PCR6aP]] | + | [[Image:PCR6aP.jpg]] |
PCR6b: replication of LctO with mutagenisis | PCR6b: replication of LctO with mutagenisis | ||
| - | [[Image:PCR6bP]] | + | [[Image:PCR6bP.jpg]] |
PCR7: Joining PCR of LctO | PCR7: Joining PCR of LctO | ||
| - | [[Image: PCR7P]] | + | [[Image: PCR7P.jpg]] |
PCR8a: replication of LctO with mutagenisis | PCR8a: replication of LctO with mutagenisis | ||
| - | [[Image:PCR8aP]] | + | [[Image:PCR8aP.jpg]] |
PCR8b: replication of LctO with mutagenisis | PCR8b: replication of LctO with mutagenisis | ||
| - | [[Image:PCR8bP]] | + | [[Image:PCR8bP.jpg]] |
PCR9: Joining PCR of LctO | PCR9: Joining PCR of LctO | ||
| - | [[Image:PCR9P]] | + | [[Image:PCR9P.jpg]] |
PCR10: Joining PCR of AurF and PduD | PCR10: Joining PCR of AurF and PduD | ||
| - | [[Image:PCR10P]] | + | [[Image:PCR10P.jpg]] |
PCR11: Joining PCR of LctO and PduC | PCR11: Joining PCR of LctO and PduC | ||
| - | [[Image:PCR11P]] | + | [[Image:PCR11P.jpg]] |
PCR12a: replication of the Pdu-Operon with mutagenisis | PCR12a: replication of the Pdu-Operon with mutagenisis | ||
| - | [[Image:PCR12aP]] | + | [[Image:PCR12aP.jpg]] |
PCR12b: replication of the Pdu-Operon with mutagenisis | PCR12b: replication of the Pdu-Operon with mutagenisis | ||
| - | [[Image:PCR12bP]] | + | [[Image:PCR12bP.jpg]] |
PCR13: Joining PCR of the Pdu-Operon | PCR13: Joining PCR of the Pdu-Operon | ||
| - | [[Image:PCR131517P]] | + | [[Image:PCR131517P.jpg]] |
PCR14a: replication of the Pdu-Operon with mutagenisis | PCR14a: replication of the Pdu-Operon with mutagenisis | ||
| - | [[Image:PCR14aP]] | + | [[Image:PCR14aP.jpg]] |
PCR14b: replication of the Pdu-Operon with mutagenisis | PCR14b: replication of the Pdu-Operon with mutagenisis | ||
| - | [[Image:PCR14bP]] | + | [[Image:PCR14bP.jpg]] |
PCR15: Joining PCR of the Pdu-Operon | PCR15: Joining PCR of the Pdu-Operon | ||
| - | [[Image:PCR131517P]] | + | [[Image:PCR131517P.jpg]] |
PCR16a: replication of the Pdu-Operon with mutagenisis | PCR16a: replication of the Pdu-Operon with mutagenisis | ||
| - | [[Image:PCR16aP]] | + | [[Image:PCR16aP.jpg]] |
PCR16b: replication of the Pdu-Operon with mutagenisis | PCR16b: replication of the Pdu-Operon with mutagenisis | ||
| - | [[Image:PCR16bP]] | + | [[Image:PCR16bP.jpg]] |
PCR17: Joining PCR of the Pdu-Operon | PCR17: Joining PCR of the Pdu-Operon | ||
| - | [[Image:PCR131517P]] | + | [[Image:PCR131517P.jpg]] |
==In Vitro testing of the chemical kinetics with the enzymes== | ==In Vitro testing of the chemical kinetics with the enzymes== | ||
Revision as of 11:16, 20 August 2010
PCR1a: replication of Pdu D with mutagenisis
PCR1b: replication of Pdu D with mutagenisis
PCR2: Joining PCR of PduD
PCR3a: replication of Pdu C with mutagenisis
PCR3b: replication of Pdu C with mutagenisis
PCR4: Joining PCR of Pdu C
PCR5: replication of AurF to be joined with Pdu D
PCR6a: replication of LctO with mutagenisis
PCR6b: replication of LctO with mutagenisis
PCR7: Joining PCR of LctO
PCR8a: replication of LctO with mutagenisis
PCR8b: replication of LctO with mutagenisis
PCR9: Joining PCR of LctO
PCR10: Joining PCR of AurF and PduD
PCR11: Joining PCR of LctO and PduC
PCR12a: replication of the Pdu-Operon with mutagenisis
PCR12b: replication of the Pdu-Operon with mutagenisis
PCR13: Joining PCR of the Pdu-Operon
PCR14a: replication of the Pdu-Operon with mutagenisis
PCR14b: replication of the Pdu-Operon with mutagenisis
PCR15: Joining PCR of the Pdu-Operon
PCR16a: replication of the Pdu-Operon with mutagenisis
PCR16b: replication of the Pdu-Operon with mutagenisis
PCR17: Joining PCR of the Pdu-Operon
Contents
PCR and mutagenisis of the digestion sites
In Vitro testing of the chemical kinetics with the enzymes
Transforming the construct in bacteria
Testing the system
