Team:LMU-Munich/Notebook/Protocols/8 SOC medium
From 2010.igem.org
(Difference between revisions)
(New page: {{:Team:LMU-Munich/Templates/Page Header}} Source: http://partsregistry.org/SOC == Summary == SOC Medium. Used in bacterial transformation. == Ingredients == *SOB *20 mM glucose ==...) |
|||
Line 5: | Line 5: | ||
== Ingredients == | == Ingredients == | ||
- | *[[SOB]] | + | *[[Team:LMU-Munich/Notebook/Protocols/6_SOB_medium|SOB]] |
*20 mM glucose | *20 mM glucose | ||
==Protocol== | ==Protocol== | ||
- | #Follow directions to make 1 liter of [[SOB]] media | + | #Follow directions to make 1 liter of [[Team:LMU-Munich/Notebook/Protocols/6_SOB_medium|SOB]] media |
#After cooling medium to less than 50°C, add 20 ml filter sterilized 20% glucose solution | #After cooling medium to less than 50°C, add 20 ml filter sterilized 20% glucose solution | ||
Latest revision as of 13:19, 13 August 2010
SOC Medium. Used in bacterial transformation.
Adapted From:
F. Ausubel et al., Short Protocols in Molecular Biology (John Wiley & Sons, ed. 4, 1999) pg. A1-36
The "C" in SOC supposedly stands for catabolite repression due to the added glucose.
Source: http://partsregistry.org/SOC
Contents
Summary
Ingredients
Protocol
Source
Trivia