Team:LMU-Munich/Notebook/Protocols/17 Special thermal cycler programs/Touch down phusion

From 2010.igem.org


PCR program: Touch-Down with Phusion

98°C 1 min
98°C 10 sec
59°C 20 sec
73°C 20 sec
return to step 2 for 2 cycles
98°C 10 sec
57°C 20 sec
73°C 20 sec
return to step 6 for 2 cycles
98°C 10 sec
55°C 20 sec
73°C 20 sec
return to step 10 for 2 cycles
98°C 10 sec
53°C 20 sec
73°C 20 sec
return to step 14 for 2 cycles
98°C 10 sec
51°C 20 sec
73°C 20 sec
return to step 18 for 2 cycles
98°C 10 sec
49°C 20 sec
73°C 20 sec
return to step 22 for 2 cycles
95°C 30 sec
53°C 30 sec
72°C 30 sec
return to step 26 for 29 cycles
73°C 10 min
12°C forever