Team:LMU-Munich/Jump-or-die/Schedule/SequenzPCR3

From 2010.igem.org

(Difference between revisions)
(Sequence PCR3)
(Sequence PCR3)
 
Line 7: Line 7:
=== with reverse primer (primer 6)===
=== with reverse primer (primer 6)===
-
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
+
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNANNNNNNNNN
 +
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
Line 13: Line 14:
=== with forward primer===
=== with forward primer===
-
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTTNNAGAGANNGATGAATTGNTNAGGNNGNNNTNTTCTTTNTACAAGAAGGCTGGTTCTGAAAACTTGTACTTCCAATTCCACAAGTCTGGTGCTTGGAANNTGACANTTTCTTNGGTTAANANAGGGATCGATAAGCTTGATTATAAANAACANCTTCNGGCTTGGCGGTGGGAAAGAGAAATTGATGANAGAANTCGCCCACTTTCTGATGANGAATTAGATGCTATGTTCCCANAANGATATAAGGTACTTCCTCCTNCAGCTGGTTATGTTCNTATTCGAACTCCANCTCATATGGATCGATTCCNGCANNAGCANGTGCTGGTGCTGAAGTTGCTGTTGCNAGNCNNGNGATTNACCACCNAAATTANGTCATTTAGGANCNTACTANCACCCCCCTCCTGCNNTCCNGCAAANNN
+
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTTNNAGAGANNGATGAATTG
 +
 
 +
NTNAGGNNGNNNTNTTCTTTNTACAAGAAGGCTGGTTCTGAAAACTTGTACTTCCA
 +
 
 +
ATTCCACAAGTCTGGTGCTTGGAANNTGACANTTTCTTNGGTTAANANAGGGATCGA
 +
 
 +
TAAGCTTGATTATAAANAACANCTTCNGGCTTGGCGGTGGGAAAGAGAAATTGATG
 +
 
 +
ANAGAANTCGCCCACTTTCTGATGANGAATTAGATGCTATGTTCCCANAANGATATA
 +
 
 +
AGGTACTTCCTCCTNCAGCTGGTTATGTTCNTATTCGAACTCCANCTCATATGGAT
 +
 
 +
CGATTCCNGCANNAGCANGTGCTGGTGCTGAAGTTGCTGTTGCNAGNCNNGNGATTN
 +
 
 +
ACCACCNAAATTANGTCATTTAGGANCNTACTANCACCCCCCTCCTGCNNTCCNGC
 +
 
 +
AAANNN
=== with reverse primer===
=== with reverse primer===
-
NNNNNNNNNNNNNNNNNNNGACCNNNCGCAGCGAGTCAGTGAGNNAGGAAGCCTGCATAACGCGAAGTAATCTTTTCGGTTTNAANGTGNNGGCAGGGTGGTGACACCTTGCCCTTTTTTGCCGGATTGNAGCGGNCGCTACTAGTAGGATCCTTTATGACCTAATTTAGATGGTAAATCACAATATCTAGCAACAGCAACTTCAGCNCCAGCACCTGCTACTGCCGGAATCGATCCATATGAGCTGGAGTTCGAATAGGAACATAACCAGCTGGAGGAGGAAGTACCTTATATCCTTCTGGGAACATAGCATCTAATTCCTCATCAGAAAGTGGGCGATTTCTCTCATCAATTTCTCTTTCCCACCGCCAAGCCTGAAGCTGTTCTTTATAATCAAGCTTATCGATCCCTCTCTTAACCAAAGAAACTGGCAACTTCCAAGCACCAGACTTGTGGAATTGGAAGTACAAGTTTTCAGAACCAGCCTTCTTGTACAAAGAAGTAATAGATTTGTACAATTCATCCATCTCTAGAAGCGGCCGCGAATTCCANAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATTCTGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGATAATAATGGTTTCTTAGACGTCAGGTGGCACTCGAGTTGATCGGGCACGTAAGAGGTTCCAACTTTCACCATAATGAAATAAGATCACTACCGGGCGTATTTTTTGAGTTATCGAGATTTTCANGAGCTAANGANCTAAATGGAGAAAAAATCACTGGATATANCACCGTTGATATATCCCAATGGCATCGTAAAGAACANTTTGAGGCATTTCAGTCAGTTGCTCNATGTACCTATAACCAGACCGTCANCTGGATNTACGGCCTTTTTANAGACCGNAAGAAAATAAGCACAAGTTTATCCGGCTTATCACATTCCTGCNGCNNGATGAATGCTCATCCGGAATTTCNATGGCNATGAAAGACGGNGANCTGGTGATATGGGNNANNGNTNNNCCTNGNNNACCNTTTCCNNNNNNAACTGNANN
+
NNNNNNNNNNNNNNNNNNNGACCNNNCGCAGCGAGTCAGTGAGNNAGGAAGCCTGCA
 +
 
 +
TAACGCGAAGTAATCTTTTCGGTTTNAANGTGNNGGCAGGGTGGTGACACCTTGCCC
 +
 
 +
TTTTTTGCCGGATTGNAGCGGNCGCTACTAGTAGGATCCTTTATGACCTAATTTAGA
 +
 
 +
TGGTAAATCACAATATCTAGCAACAGCAACTTCAGCNCCAGCACCTGCTACTGCCGG
 +
 
 +
AATCGATCCATATGAGCTGGAGTTCGAATAGGAACATAACCAGCTGGAGGAGGAAGT
 +
 
 +
ACCTTATATCCTTCTGGGAACATAGCATCTAATTCCTCATCAGAAAGTGGGCGATT
 +
 
 +
TCTCTCATCAATTTCTCTTTCCCACCGCCAAGCCTGAAGCTGTTCTTTATAATCAAG
 +
 
 +
CTTATCGATCCCTCTCTTAACCAAAGAAACTGGCAACTTCCAAGCACCAGACTTGTG
 +
 
 +
GAATTGGAAGTACAAGTTTTCAGAACCAGCCTTCTTGTACAAAGAAGTAATAGATTTG
 +
 
 +
TACAATTCATCCATCTCTAGAAGCGGCCGCGAATTCCANAAATCATCCTTAGCGAAAG
 +
 
 +
CTAAGGATTTTTTTTATCTGAAATTCTGCCTCGTGATACGCCTATTTTTATAGGTTAA
 +
 
 +
TGTCATGATAATAATGGTTTCTTAGACGTCAGGTGGCACTCGAGTTGATCGGGCACGT
 +
 
 +
AAGAGGTTCCAACTTTCACCATAATGAAATAAGATCACTACCGGGCGTATTTTTTGA
 +
 
 +
GTTATCGAGATTTTCANGAGCTAANGANCTAAATGGAGAAAAAATCACTGGATATANC
 +
 
 +
ACCGTTGATATATCCCAATGGCATCGTAAAGAACANTTTGAGGCATTTCAGTCAGTTG
 +
 
 +
CTCNATGTACCTATAACCAGACCGTCANCTGGATNTACGGCCTTTTTANAGACCGNAA
 +
 
 +
GAAAATAAGCACAAGTTTATCCGGCTTATCACATTCCTGCNGCNNGATGAATGCTCAT
 +
 
 +
CCGGAATTTCNATGGCNATGAAAGACGGNGANCTGGTGATATGGGNNANNGNTNNNCC
 +
 
 +
TNGNNNACCNTTTCCNNNNNNAACTGNANN
{{:Team:LMU-Munich/Templates/Page Footer}}
{{:Team:LMU-Munich/Templates/Page Footer}}

Latest revision as of 17:23, 24 October 2010


Contents

Sequence PCR3

with forward primer (primer 3)

NNNNN


with reverse primer (primer 6)

GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNANNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN


verified by sequencing of BBa_K368016 (PCR 3 in pSB1C3)

with forward primer

NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGTTNNAGAGANNGATGAATTG

NTNAGGNNGNNNTNTTCTTTNTACAAGAAGGCTGGTTCTGAAAACTTGTACTTCCA

ATTCCACAAGTCTGGTGCTTGGAANNTGACANTTTCTTNGGTTAANANAGGGATCGA

TAAGCTTGATTATAAANAACANCTTCNGGCTTGGCGGTGGGAAAGAGAAATTGATG

ANAGAANTCGCCCACTTTCTGATGANGAATTAGATGCTATGTTCCCANAANGATATA

AGGTACTTCCTCCTNCAGCTGGTTATGTTCNTATTCGAACTCCANCTCATATGGAT

CGATTCCNGCANNAGCANGTGCTGGTGCTGAAGTTGCTGTTGCNAGNCNNGNGATTN

ACCACCNAAATTANGTCATTTAGGANCNTACTANCACCCCCCTCCTGCNNTCCNGC

AAANNN

with reverse primer

NNNNNNNNNNNNNNNNNNNGACCNNNCGCAGCGAGTCAGTGAGNNAGGAAGCCTGCA

TAACGCGAAGTAATCTTTTCGGTTTNAANGTGNNGGCAGGGTGGTGACACCTTGCCC

TTTTTTGCCGGATTGNAGCGGNCGCTACTAGTAGGATCCTTTATGACCTAATTTAGA

TGGTAAATCACAATATCTAGCAACAGCAACTTCAGCNCCAGCACCTGCTACTGCCGG

AATCGATCCATATGAGCTGGAGTTCGAATAGGAACATAACCAGCTGGAGGAGGAAGT

ACCTTATATCCTTCTGGGAACATAGCATCTAATTCCTCATCAGAAAGTGGGCGATT

TCTCTCATCAATTTCTCTTTCCCACCGCCAAGCCTGAAGCTGTTCTTTATAATCAAG

CTTATCGATCCCTCTCTTAACCAAAGAAACTGGCAACTTCCAAGCACCAGACTTGTG

GAATTGGAAGTACAAGTTTTCAGAACCAGCCTTCTTGTACAAAGAAGTAATAGATTTG

TACAATTCATCCATCTCTAGAAGCGGCCGCGAATTCCANAAATCATCCTTAGCGAAAG

CTAAGGATTTTTTTTATCTGAAATTCTGCCTCGTGATACGCCTATTTTTATAGGTTAA

TGTCATGATAATAATGGTTTCTTAGACGTCAGGTGGCACTCGAGTTGATCGGGCACGT

AAGAGGTTCCAACTTTCACCATAATGAAATAAGATCACTACCGGGCGTATTTTTTGA

GTTATCGAGATTTTCANGAGCTAANGANCTAAATGGAGAAAAAATCACTGGATATANC

ACCGTTGATATATCCCAATGGCATCGTAAAGAACANTTTGAGGCATTTCAGTCAGTTG

CTCNATGTACCTATAACCAGACCGTCANCTGGATNTACGGCCTTTTTANAGACCGNAA

GAAAATAAGCACAAGTTTATCCGGCTTATCACATTCCTGCNGCNNGATGAATGCTCAT

CCGGAATTTCNATGGCNATGAAAGACGGNGANCTGGTGATATGGGNNANNGNTNNNCC

TNGNNNACCNTTTCCNNNNNNAACTGNANN