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Team:LMU-Munich/Jump-or-die/Schedule
From 2010.igem.org
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==Aim 1: DNA reproduction+PCR== | ==Aim 1: DNA reproduction+PCR== | ||
| + | PCR1: replication of the Tet-inducible CMV promotor [X] | ||
| + | [[Image:PCR1.jpg]] | ||
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| + | Primer used:[[Team:LMU-Munich/Cut'N'survive/Schedule/Primer1| 1TreF]][[Team:LMU-Munich/Cut'N'survive/Schedule/Primer2| 2TreR]] | ||
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| + | PCR4a: replication human bak with mutagenisis (Pst1) [ ] | ||
| + | [[Image:PCR4a.jpg]] | ||
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| + | Primer used:[[Team:LMU-Munich/Cut'N'survive/Schedule/Primer7| 7BakF]][[Team:LMU-Munich/Cut'N'survive/Schedule/Primer8| 8BakMutPR]] | ||
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| + | PCR4b: replication human bak with mutagenisis (Pst1) [ ] | ||
| + | [[Image:PCR4b.jpg]] | ||
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| + | Primer used:[[Team:LMU-Munich/Cut'N'survive/Schedule/Primer9| 9BakMutPF]][[Team:LMU-Munich/Cut'N'survive/Schedule/Primer10| 10BakR]] | ||
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| + | PCR5: joining PCR of human bak [ ] | ||
| + | [[Image:PCR5.jpg]] | ||
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| + | Primer used:[[Team:LMU-Munich/Cut'N'survive/Schedule/Primer7| 7BakF]][[Team:LMU-Munich/Cut'N'survive/Schedule/Primer10| 10BakR]] | ||
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| + | PCR6: replication of the SV40-polyadenylation site [X] | ||
| + | [[Image:PCR6.jpg]] | ||
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| + | Primer used:[[Team:LMU-Munich/Cut'N'survive/Schedule/Primer11| 11PAF]][[Team:LMU-Munich/Cut'N'survive/Schedule/Primer12| 12PAR]] | ||
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==Aim 2: Assembling Biobricks== | ==Aim 2: Assembling Biobricks== | ||
==Aim 3: Testing products== | ==Aim 3: Testing products== | ||
Revision as of 13:12, 18 August 2010
PCR1: replication of the Tet-inducible CMV promotor [X]
PCR4a: replication human bak with mutagenisis (Pst1) [ ]
PCR4b: replication human bak with mutagenisis (Pst1) [ ]
PCR5: joining PCR of human bak [ ]
PCR6: replication of the SV40-polyadenylation site [X]
Contents
Aim 1: DNA reproduction+PCR
Aim 2: Assembling Biobricks
Aim 3: Testing products
Aim 4: Testing system
