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Team:LMU-Munich/Jump-or-die/Schedule
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(→Aim 2: Assembling Biobricks) |
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==Aim 2: Assembling Biobricks== | ==Aim 2: Assembling Biobricks== | ||
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| + | We are using the 3A System to assemble Biobricks. | ||
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| + | <b> Assembling Construct 1</b> | ||
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| + | [[Image: J1.jpg]] | ||
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| + | <b> Assembling Construct 2</b> | ||
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| + | [[Image: J2.jpg]] | ||
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| + | <b> Assembling Construct 3</b> | ||
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| + | [[Image: J3.jpg]] | ||
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==Aim 3: Testing products== | ==Aim 3: Testing products== | ||
==Aim 4: Testing system== | ==Aim 4: Testing system== | ||
{{:Team:LMU-Munich/Templates/Page Footer}} | {{:Team:LMU-Munich/Templates/Page Footer}} | ||
Revision as of 16:02, 20 August 2010
For PCR key see PCR key
Colourcode for the primers: Annealing part, mutagenised part, other sequences integrated into primer
Primer used: 1TreF 2TreR
expected product size: 492 bp
Primer used: 7BakF 8BakMutPR
expected product size: 330 bp
Primer used: 9BakMutPF 10BakR
expected product size: 376 bp
Primer used: 7BakF 10BakR
expected product size: 688 bp
Primer used: 11PAF 12PAR
expected product size: 237 bp
Primer used: 20eGFPattPF 21eGFPR
expected product size: 808 bp
Primer used: 22PC31oF 23PC31oR
expected product size: 1888 bp
Note: for attB two Primers ( 18attBF 19attBR) used.
We are using the 3A System to assemble Biobricks.
Assembling Construct 1
Assembling Construct 2
Assembling Construct 3
Contents
Aim 1: DNA reproduction+PCR
PCR1: replication of the Tet-inducible CMV promotor [X]
PCR4a: replication human bak with mutagenesis (Pst1) [ ]
PCR4b: replication human bak with mutagenesis (Pst1) [ ]
PCR5: joining PCR of human bak [ ]
PCR6: replication of the SV40-polyadenylation site [X]
PCR9: replication of eGFP with attP in primer [ ]
PCR10: Replikation of PhiC31o [ ]
Aim 2: Assembling Biobricks
Aim 3: Testing products
Aim 4: Testing system
