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Team:LMU-Munich/Jump-or-die/Schedule
From 2010.igem.org
(→Aim 1: DNA reproduction+PCR) |
(→Aim 1: DNA reproduction+PCR) |
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Primer used:[[Team:LMU-Munich/Cut'N'survive/Schedule/Primer1| 1TreF]][[Team:LMU-Munich/Cut'N'survive/Schedule/Primer2| 2TreR]] | Primer used:[[Team:LMU-Munich/Cut'N'survive/Schedule/Primer1| 1TreF]][[Team:LMU-Munich/Cut'N'survive/Schedule/Primer2| 2TreR]] | ||
| - | expected product size: | + | expected product size: 492 bp |
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Primer used:[[Team:LMU-Munich/Cut'N'survive/Schedule/Primer7| 7BakF]][[Team:LMU-Munich/Cut'N'survive/Schedule/Primer8| 8BakMutPR]] | Primer used:[[Team:LMU-Munich/Cut'N'survive/Schedule/Primer7| 7BakF]][[Team:LMU-Munich/Cut'N'survive/Schedule/Primer8| 8BakMutPR]] | ||
| - | expected product size: | + | expected product size: 330 bp |
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Primer used:[[Team:LMU-Munich/Cut'N'survive/Schedule/Primer9| 9BakMutPF]][[Team:LMU-Munich/Cut'N'survive/Schedule/Primer10| 10BakR]] | Primer used:[[Team:LMU-Munich/Cut'N'survive/Schedule/Primer9| 9BakMutPF]][[Team:LMU-Munich/Cut'N'survive/Schedule/Primer10| 10BakR]] | ||
| - | expected product size: | + | expected product size: 376 bp |
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Primer used:[[Team:LMU-Munich/Cut'N'survive/Schedule/Primer7| 7BakF]][[Team:LMU-Munich/Cut'N'survive/Schedule/Primer10| 10BakR]] | Primer used:[[Team:LMU-Munich/Cut'N'survive/Schedule/Primer7| 7BakF]][[Team:LMU-Munich/Cut'N'survive/Schedule/Primer10| 10BakR]] | ||
| - | expected product size: | + | expected product size: 688 bp |
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Primer used:[[Team:LMU-Munich/Cut'N'survive/Schedule/Primer11| 11PAF]][[Team:LMU-Munich/Cut'N'survive/Schedule/Primer12| 12PAR]] | Primer used:[[Team:LMU-Munich/Cut'N'survive/Schedule/Primer11| 11PAF]][[Team:LMU-Munich/Cut'N'survive/Schedule/Primer12| 12PAR]] | ||
| - | expected product size: | + | expected product size: 237 bp |
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Primer used:[[Team:LMU-Munich/Cut'N'survive/Schedule/Primer20| 20eGFPattPF]][[Team:LMU-Munich/Cut'N'survive/Schedule/Primer21| 21eGFPR]] | Primer used:[[Team:LMU-Munich/Cut'N'survive/Schedule/Primer20| 20eGFPattPF]][[Team:LMU-Munich/Cut'N'survive/Schedule/Primer21| 21eGFPR]] | ||
| - | expected product size: | + | expected product size: 1213 bp |
Revision as of 11:45, 20 August 2010
For PCR key see PCR key
Colourcode for the primers: Annealing part, mutagenised part, other sequences integrated into primer
Primer used: 1TreF 2TreR
expected product size: 492 bp
Primer used: 7BakF 8BakMutPR
expected product size: 330 bp
Primer used: 9BakMutPF 10BakR
expected product size: 376 bp
Primer used: 7BakF 10BakR
expected product size: 688 bp
Primer used: 11PAF 12PAR
expected product size: 237 bp
Primer used: 20eGFPattPF 21eGFPR
expected product size: 1213 bp
Primer used: 22PC31oF 23PC31oR
expected product size: bp
Note: for attB two Primers ( 18attBF 19attBR) used.
Contents
Aim 1: DNA reproduction+PCR
PCR1: replication of the Tet-inducible CMV promotor [X]
PCR4a: replication human bak with mutagenesis (Pst1) [ ]
PCR4b: replication human bak with mutagenesis (Pst1) [ ]
PCR5: joining PCR of human bak [ ]
PCR6: replication of the SV40-polyadenylation site [X]
PCR9: replication of eGFP with attP in primer [ ]
PCR10: Replikation of PhiC31o [ ]
Aim 2: Assembling Biobricks
Aim 3: Testing products
Aim 4: Testing system
