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Team:LMU-Munich/Jump-or-die/Schedule
From 2010.igem.org
(→Aim 1: DNA reproduction+PCR) |
(→Aim 1: DNA reproduction+PCR) |
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<b>Colourcode for the primers: <font color="#FF0000">Annealing part</font>, <font color="#009933">mutagenised part</font>, <font color="#FF6600">other sequences integrated into primer</font></b> | <b>Colourcode for the primers: <font color="#FF0000">Annealing part</font>, <font color="#009933">mutagenised part</font>, <font color="#FF6600">other sequences integrated into primer</font></b> | ||
| - | PCR1: replication of the Tet-inducible CMV promotor [X] | + | |
| + | <b>PCR1: replication of the Tet-inducible CMV promotor [X]</b> | ||
[[Image:PCR1.jpg]] | [[Image:PCR1.jpg]] | ||
Primer used:[[Team:LMU-Munich/Cut'N'survive/Schedule/Primer1| 1TreF]][[Team:LMU-Munich/Cut'N'survive/Schedule/Primer2| 2TreR]] | Primer used:[[Team:LMU-Munich/Cut'N'survive/Schedule/Primer1| 1TreF]][[Team:LMU-Munich/Cut'N'survive/Schedule/Primer2| 2TreR]] | ||
| + | expected product size: bp | ||
| - | PCR4a: replication human bak with mutagenesis (Pst1) [ ] | + | |
| + | <b>PCR4a: replication human bak with mutagenesis (Pst1) [ ]</b> | ||
[[Image:PCR4a.jpg]] | [[Image:PCR4a.jpg]] | ||
Primer used:[[Team:LMU-Munich/Cut'N'survive/Schedule/Primer7| 7BakF]][[Team:LMU-Munich/Cut'N'survive/Schedule/Primer8| 8BakMutPR]] | Primer used:[[Team:LMU-Munich/Cut'N'survive/Schedule/Primer7| 7BakF]][[Team:LMU-Munich/Cut'N'survive/Schedule/Primer8| 8BakMutPR]] | ||
| + | expected product size: bp | ||
| - | PCR4b: replication human bak with mutagenesis (Pst1) [ ] | + | |
| + | <b>PCR4b: replication human bak with mutagenesis (Pst1) [ ]</b> | ||
[[Image:PCR4b.jpg]] | [[Image:PCR4b.jpg]] | ||
Primer used:[[Team:LMU-Munich/Cut'N'survive/Schedule/Primer9| 9BakMutPF]][[Team:LMU-Munich/Cut'N'survive/Schedule/Primer10| 10BakR]] | Primer used:[[Team:LMU-Munich/Cut'N'survive/Schedule/Primer9| 9BakMutPF]][[Team:LMU-Munich/Cut'N'survive/Schedule/Primer10| 10BakR]] | ||
| + | expected product size: bp | ||
| - | PCR5: joining PCR of human bak [ ] | + | |
| + | <b>PCR5: joining PCR of human bak [ ]</b> | ||
[[Image:PCR5.jpg]] | [[Image:PCR5.jpg]] | ||
Primer used:[[Team:LMU-Munich/Cut'N'survive/Schedule/Primer7| 7BakF]][[Team:LMU-Munich/Cut'N'survive/Schedule/Primer10| 10BakR]] | Primer used:[[Team:LMU-Munich/Cut'N'survive/Schedule/Primer7| 7BakF]][[Team:LMU-Munich/Cut'N'survive/Schedule/Primer10| 10BakR]] | ||
| + | expected product size: bp | ||
| - | PCR6: replication of the SV40-polyadenylation site [X] | + | |
| + | <b>PCR6: replication of the SV40-polyadenylation site [X]</b> | ||
[[Image:PCR6.jpg]] | [[Image:PCR6.jpg]] | ||
Primer used:[[Team:LMU-Munich/Cut'N'survive/Schedule/Primer11| 11PAF]][[Team:LMU-Munich/Cut'N'survive/Schedule/Primer12| 12PAR]] | Primer used:[[Team:LMU-Munich/Cut'N'survive/Schedule/Primer11| 11PAF]][[Team:LMU-Munich/Cut'N'survive/Schedule/Primer12| 12PAR]] | ||
| + | expected product size: bp | ||
| - | PCR9: replication of eGFP with attP in primer [ ] | + | |
| + | <b>PCR9: replication of eGFP with attP in primer [ ]</b> | ||
[[Image:PCR9.jpg]] | [[Image:PCR9.jpg]] | ||
Primer used:[[Team:LMU-Munich/Cut'N'survive/Schedule/Primer20| 20eGFPattPF]][[Team:LMU-Munich/Cut'N'survive/Schedule/Primer21| 21eGFPR]] | Primer used:[[Team:LMU-Munich/Cut'N'survive/Schedule/Primer20| 20eGFPattPF]][[Team:LMU-Munich/Cut'N'survive/Schedule/Primer21| 21eGFPR]] | ||
| + | expected product size: bp | ||
| + | |||
| - | PCR10: Replikation of PhiC31o [ ] | + | <b>PCR10: Replikation of PhiC31o [ ]</b> |
[[Image:PCR10.jpg]] | [[Image:PCR10.jpg]] | ||
Primer used:[[Team:LMU-Munich/Cut'N'survive/Schedule/Primer22| 22PC31oF]][[Team:LMU-Munich/Cut'N'survive/Schedule/Primer23| 23PC31oR]] | Primer used:[[Team:LMU-Munich/Cut'N'survive/Schedule/Primer22| 22PC31oF]][[Team:LMU-Munich/Cut'N'survive/Schedule/Primer23| 23PC31oR]] | ||
| + | expected product size: bp | ||
| - | Note: for attB two Primers ([[Team:LMU-Munich/Cut'N'survive/Schedule/Primer18| 18attBF]][[Team:LMU-Munich/Cut'N'survive/Schedule/Primer19| 19attBR]]) used. | + | <b>Note: for attB two Primers ([[Team:LMU-Munich/Cut'N'survive/Schedule/Primer18| 18attBF]][[Team:LMU-Munich/Cut'N'survive/Schedule/Primer19| 19attBR]]) used.</b> |
==Aim 2: Assembling Biobricks== | ==Aim 2: Assembling Biobricks== | ||
Revision as of 11:41, 20 August 2010
For PCR key see PCR key
Colourcode for the primers: Annealing part, mutagenised part, other sequences integrated into primer
Primer used: 1TreF 2TreR
expected product size: bp
Primer used: 7BakF 8BakMutPR
expected product size: bp
Primer used: 9BakMutPF 10BakR
expected product size: bp
Primer used: 7BakF 10BakR
expected product size: bp
Primer used: 11PAF 12PAR
expected product size: bp
Primer used: 20eGFPattPF 21eGFPR
expected product size: bp
Primer used: 22PC31oF 23PC31oR
expected product size: bp
Note: for attB two Primers ( 18attBF 19attBR) used.
Contents
Aim 1: DNA reproduction+PCR
PCR1: replication of the Tet-inducible CMV promotor [X]
PCR4a: replication human bak with mutagenesis (Pst1) [ ]
PCR4b: replication human bak with mutagenesis (Pst1) [ ]
PCR5: joining PCR of human bak [ ]
PCR6: replication of the SV40-polyadenylation site [X]
PCR9: replication of eGFP with attP in primer [ ]
PCR10: Replikation of PhiC31o [ ]
Aim 2: Assembling Biobricks
Aim 3: Testing products
Aim 4: Testing system
