Team:LMU-Munich/Jump-or-die/Schedule

From 2010.igem.org

(Difference between revisions)
(Aim 1: DNA reproduction+PCR)
(Aim 1: DNA reproduction+PCR)
Line 2: Line 2:
==Aim 1: DNA reproduction+PCR==
==Aim 1: DNA reproduction+PCR==
 +
 +
For PCR key see [[Team:LMU-Munich/Cut'N'survive/Schedule/PCR key | PCR key]]
 +
<b>Colourcode for the primers: <font color="#FF0000">Annealing part</font>, <font color="#009933">mutagenised part</font>, <font color="#FF6600">other sequences integrated into primer</font></b>
<b>Colourcode for the primers: <font color="#FF0000">Annealing part</font>, <font color="#009933">mutagenised part</font>, <font color="#FF6600">other sequences integrated into primer</font></b>

Revision as of 14:44, 18 August 2010


Contents

Aim 1: DNA reproduction+PCR

For PCR key see PCR key

Colourcode for the primers: Annealing part, mutagenised part, other sequences integrated into primer

PCR1: replication of the Tet-inducible CMV promotor [X] PCR1.jpg

Primer used: 1TreF 2TreR

PCR4a: replication human bak with mutagenesis (Pst1) [ ] PCR4a.jpg

Primer used: 7BakF 8BakMutPR

PCR4b: replication human bak with mutagenesis (Pst1) [ ] PCR4b.jpg

Primer used: 9BakMutPF 10BakR

PCR5: joining PCR of human bak [ ] PCR5.jpg

Primer used: 7BakF 10BakR

PCR6: replication of the SV40-polyadenylation site [X] PCR6.jpg

Primer used: 11PAF 12PAR

PCR9: replication of eGFP with attP in primer [ ] PCR9.jpg

Primer used: 20eGFPattPF 21eGFPR

PCR10: Replikation of PhiC31o [ ] PCR10.jpg

Primer used: 22PC31oF 23PC31oR

Note: for attB two Primers ( 18attBF 19attBR) used.

Aim 2: Assembling Biobricks

Aim 3: Testing products

Aim 4: Testing system