Team:LMU-Munich/Jump-or-die/Functional Principle
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(Difference between revisions)
(New page: {{:Team:LMU-Munich/Templates/Page Header}} == Jump-or-die-System == The jump-or-die-system selects the itegration of a gene of interest by apoptosis. Therefore three constructs ae needed....) |
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This Biobrick is containing CMV-promoter, PhiC31o (mouse-codon optimized integrase) and SV40PA. It will be transfected to the cellline and read-off so that PhiC31o will be existing. | This Biobrick is containing CMV-promoter, PhiC31o (mouse-codon optimized integrase) and SV40PA. It will be transfected to the cellline and read-off so that PhiC31o will be existing. | ||
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+ | [[Image: Jump3.jpg | 400px | Jump construct 3]] | ||
== Transfection possibilities == | == Transfection possibilities == | ||
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PhiC31o will be read off because of CMV promoter. Now the integrase will combine attB and attP site and integrate construct 2 into genom. Up next, the gene of interest is behind tet-on promoter and will be read off after induction. The doubled stop codon will efficiently prevent the tranlation of bak. This cell will express the gene of interest and survive. | PhiC31o will be read off because of CMV promoter. Now the integrase will combine attB and attP site and integrate construct 2 into genom. Up next, the gene of interest is behind tet-on promoter and will be read off after induction. The doubled stop codon will efficiently prevent the tranlation of bak. This cell will express the gene of interest and survive. | ||
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{{:Team:LMU-Munich/Templates/Page Footer}} | {{:Team:LMU-Munich/Templates/Page Footer}} |
Revision as of 09:48, 18 August 2010