Team:LMU-Munich/Cut'N'survive/Schedule

(Difference between revisions)

Revision as of 11:46, 20 August 2010


	Cut'N'Survive System Functional Principle Sequences Schedule Jump-or-Die System Functional Principle Sequences Schedule ProSearch System Schedule

	Functional Principle Sequences Schedule picture gallery

For PCR key see PCR key

Colourcode for the primers: Annealing part, mutagenised part, other sequences integrated into primer

PCR1: replication of the Tet-inducible CMV promotor [X]

Primer used: 1TreF 2TreR expected product size: 492bp

PCR2a: replication of the TEVrecogn-NDegron-SF3 Part with mutagenisis (EcoR1 + Pst1) [X]

Primer used: 3TevF 4TevMutEPR expected product size: 332 bp

PCR2b: replication of the TEVrecogn-NDegron-SF3 Part with mutagenisis (EcoR1 + Pst1) [X]

Primer used: 5TevMutEPF 6TevR expected product size: 772 bp

PCR3: joining PCR of the TEVrecogn-NDegron-SF3 Part [ ]

Primer used: 3TevF 6TevR expected product size: 1087 bp

PCR4a: replication human bak with mutagenisis (Pst1) [ ]

Primer used: 7BakF 8BakMutPR expected product size: 330 bp

PCR4b: replication human bak with mutagenisis (Pst1) [ ]

Primer used: 9BakMutPF 10BakR expected product size: 376 bp

PCR5: joining PCR of human bak [ ]

Primer used: 7BakF 10BakR expected product size: 688 bp

PCR6: replication of the SV40-polyadenylation site [X]

Primer used: 11PAF 12PAR expected product size: 237 bp

PCR7a: replication of the p14*TEVrecogn with mutagenisis (Spe1) [ ]

Primer used: 13TrecF 14TrecMutSR expected product size: 850 bp

PCR7b: replication of the p14*TEVrecogn with mutagenisis (Spe1) with TEV recogn in primer (16TrecR) [ ]

Primer used: 15TrecMutSF 16TrecR expected product size: 402 bp

PCR8: joining PCR of p14*TEVrecogn with TEV recogn in primer (16TrecR) [ ]

Primer used: 13TrecF 16TrecR expected product size: 1233 bp

@@ Line 47: / Line 47: @@
 Primer used:[[Team:LMU-Munich/Cut'N'survive/Schedule/Primer9| 9BakMutPF]][[Team:LMU-Munich/Cut'N'survive/Schedule/Primer10| 10BakR]]
-expected product size: 373 bp
+expected product size: 376 bp
@@ Line 54: / Line 54: @@
 Primer used:[[Team:LMU-Munich/Cut'N'survive/Schedule/Primer7| 7BakF]][[Team:LMU-Munich/Cut'N'survive/Schedule/Primer10| 10BakR]]
-expected product size: 685 bp
+expected product size: 688 bp
@@ Line 75: / Line 75: @@
 Primer used:[[Team:LMU-Munich/Cut'N'survive/Schedule/Primer15| 15TrecMutSF]][[Team:LMU-Munich/Cut'N'survive/Schedule/Primer16| 16TrecR]]
-expected product size: 382 bp
+expected product size: 402 bp
@@ Line 82: / Line 82: @@
 Primer used:[[Team:LMU-Munich/Cut'N'survive/Schedule/Primer13| 13TrecF]][[Team:LMU-Munich/Cut'N'survive/Schedule/Primer16| 16TrecR]]
-expected product size: 1213 bp
+expected product size: 1233 bp
 ==Aim 2: Assembling Biobricks==