Team:LMU-Munich/Cut'N'survive/Functional Principle
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=== Construct 2 === | === Construct 2 === | ||
- | Construct 2 is composed of CMV-promoter, TEV-protease, p14, TEV-recognition site, eGFP | + | Construct 2 is composed of CMV-promoter, TEV-protease, p14, TEV-recognition site, eGFP, a double stop codon and SV40PA. |
- | [[Image: | + | [[Image:TEV12.jpg|400pxs|TEV Construct 2: Contains gene of interest]] |
The CMV-promoter is to read off construct 2. The task of TEV-protease is to cut TEV-recognition sites, especially the one ahead of N-degron to signal protein degradation. p14 is interacting with SF3b155 and so increases the rate of finding the TEV-recognition site by the TEV-proteases. eGFP is our example of a gene of interest which can be verified by green flourescence. | The CMV-promoter is to read off construct 2. The task of TEV-protease is to cut TEV-recognition sites, especially the one ahead of N-degron to signal protein degradation. p14 is interacting with SF3b155 and so increases the rate of finding the TEV-recognition site by the TEV-proteases. eGFP is our example of a gene of interest which can be verified by green flourescence. |
Revision as of 12:31, 18 August 2010