http://2010.igem.org/wiki/index.php?title=Team:Kyoto/Safety&feed=atom&action=historyTeam:Kyoto/Safety - Revision history2024-03-29T13:44:16ZRevision history for this page on the wikiMediaWiki 1.16.5http://2010.igem.org/wiki/index.php?title=Team:Kyoto/Safety&diff=207523&oldid=prevTomonori: /* 1. Safety of Project */2010-10-28T03:30:24Z<p><span class="autocomment">1. Safety of Project</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* Ethidium Bromide (EtBr)---Very commonly used to visualize DNA and RNA because it binds to nucleic acids and fluoresces when exposed to UV. However, its binding property to double-stranded DNA makes itself harmful for organisms at the same time---it is thought to intercalate DNA and deform the molecule, thereby acting as a mutagen. Therefore, care should be taken during its use, storage and disposal. We wore gloves whenever we use EtBr, and all the EtBr contaminated debris were double wrapped and collected separately to be incinerated. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* Ethidium Bromide (EtBr)---Very commonly used to visualize DNA and RNA because it binds to nucleic acids and fluoresces when exposed to UV. However, its binding property to double-stranded DNA makes itself harmful for organisms at the same time---it is thought to intercalate DNA and deform the molecule, thereby acting as a mutagen. Therefore, care should be taken during its use, storage and disposal. We wore gloves whenever we use EtBr, and all the EtBr contaminated debris were double wrapped and collected separately to be incinerated. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">: </del>Ultra Violet machine---Used in combination with EtBr. As is well known, UV has various hazardous effects on human body. In addition to causing direct and indirect damage to DNA (through subtype UVB and UVC), it also damages collagen fibers, destroys vitamin A, and is especially harmful to eyes and skin. All the more because we are familiar with UV ray, we should be careful when using the machine not to over expose ourselves to it.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">* </ins>Ultra Violet machine---Used in combination with EtBr. As is well known, UV has various hazardous effects on human body. In addition to causing direct and indirect damage to DNA (through subtype UVB and UVC), it also damages collagen fibers, destroys vitamin A, and is especially harmful to eyes and skin. All the more because we are familiar with UV ray, we should be careful when using the machine not to over expose ourselves to it.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* Autoclave---Used to sterilize equipment and reagents and to inactivate bacteria. The proper use of autoclave is imperative since it works at high temperature and pressure and is therefore potentially very dangerous. Senior students confirmed and reconfirmed over and over again that we all operate it correctly.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* Autoclave---Used to sterilize equipment and reagents and to inactivate bacteria. The proper use of autoclave is imperative since it works at high temperature and pressure and is therefore potentially very dangerous. Senior students confirmed and reconfirmed over and over again that we all operate it correctly.</div></td></tr>
</table>Tomonorihttp://2010.igem.org/wiki/index.php?title=Team:Kyoto/Safety&diff=207503&oldid=prevTomonori: /* Safety */2010-10-28T03:29:59Z<p><span class="autocomment">Safety</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Q. Would any of your project ideas raise safety issues in terms of researcher safety, public safety, or environmental safety?</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Q. Would any of your project ideas raise safety issues in terms of researcher safety, public safety, or environmental safety?</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>* <del class="diffchange diffchange-inline"> </del>Public safety</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>* Public safety</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>To ensure the safety of both the public and researchers, we consulted our advisors when planning out our project. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>To ensure the safety of both the public and researchers, we consulted our advisors when planning out our project. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>In the process, we abandoned many of our ideas due to the concern that they may raise safety issues: </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>In the process, we abandoned many of our ideas due to the concern that they may raise safety issues: </div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The major materials we used that may raise safety issues include: </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The major materials we used that may raise safety issues include: </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">・Ethidium </del>Bromide (EtBr)---Very commonly used to visualize DNA and RNA because it binds to nucleic acids and fluoresces when exposed to UV. However, its binding property to double-stranded DNA makes itself harmful for organisms at the same time---it is thought to intercalate DNA and deform the molecule, thereby acting as a mutagen. Therefore, care should be taken during its use, storage and disposal. We wore gloves whenever we use EtBr, and all the EtBr contaminated debris were double wrapped and collected separately to be incinerated. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">* Ethidium </ins>Bromide (EtBr)---Very commonly used to visualize DNA and RNA because it binds to nucleic acids and fluoresces when exposed to UV. However, its binding property to double-stranded DNA makes itself harmful for organisms at the same time---it is thought to intercalate DNA and deform the molecule, thereby acting as a mutagen. Therefore, care should be taken during its use, storage and disposal. We wore gloves whenever we use EtBr, and all the EtBr contaminated debris were double wrapped and collected separately to be incinerated. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">・Ultra </del>Violet machine---Used in combination with EtBr. As is well known, UV has various hazardous effects on human body. In addition to causing direct and indirect damage to DNA (through subtype UVB and UVC), it also damages collagen fibers, destroys vitamin A, and is especially harmful to eyes and skin. All the more because we are familiar with UV ray, we should be careful when using the machine not to over expose ourselves to it.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">: Ultra </ins>Violet machine---Used in combination with EtBr. As is well known, UV has various hazardous effects on human body. In addition to causing direct and indirect damage to DNA (through subtype UVB and UVC), it also damages collagen fibers, destroys vitamin A, and is especially harmful to eyes and skin. All the more because we are familiar with UV ray, we should be careful when using the machine not to over expose ourselves to it.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">・Autoclave</del>---Used to sterilize equipment and reagents and to inactivate bacteria. The proper use of autoclave is imperative since it works at high temperature and pressure and is therefore potentially very dangerous. Senior students confirmed and reconfirmed over and over again that we all operate it correctly.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">* Autoclave</ins>---Used to sterilize equipment and reagents and to inactivate bacteria. The proper use of autoclave is imperative since it works at high temperature and pressure and is therefore potentially very dangerous. Senior students confirmed and reconfirmed over and over again that we all operate it correctly.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">・Gas </del>burner---Since we first had to do without biosafety cabinets and an alternative control measure was required, we used gas burners and heated the air around the workspace to rise, thereby reducing contamination. Although gas burner is a common combustion apparatus we are all familiar with, it can directly cause a massive disaster if used improperly. We made all-out efforts to keep all the flammable items away from the flame and to not pass behind a person who is using a gas burner. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">* Gas </ins>burner---Since we first had to do without biosafety cabinets and an alternative control measure was required, we used gas burners and heated the air around the workspace to rise, thereby reducing contamination. Although gas burner is a common combustion apparatus we are all familiar with, it can directly cause a massive disaster if used improperly. We made all-out efforts to keep all the flammable items away from the flame and to not pass behind a person who is using a gas burner. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">・Chloramphenicol </del>(Cam)---As is often the case with antibiotics, Cam has harmful effects on us. Among its adverse side effects, the two most serious may be bone marrow suppression and the resulting aplastic anemia. This year, we used Cam only for bacterial selection for part submission, but we made all of us aware of its myelotoxicity and wear gloves when handling.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">* Chloramphenicol </ins>(Cam)---As is often the case with antibiotics, Cam has harmful effects on us. Among its adverse side effects, the two most serious may be bone marrow suppression and the resulting aplastic anemia. This year, we used Cam only for bacterial selection for part submission, but we made all of us aware of its myelotoxicity and wear gloves when handling.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>* <del class="diffchange diffchange-inline"> </del>Environmental safety </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>* Environmental safety </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Since one of the purposes of our project is to prevent the unregulated spread of genetically modified organisms, especially bacteria, </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Since one of the purposes of our project is to prevent the unregulated spread of genetically modified organisms, especially bacteria, </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>the closest attention was paid when handling and disposing of bacterial cultures or any of its by-products. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>the closest attention was paid when handling and disposing of bacterial cultures or any of its by-products. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We made sure all the biohazardous waste and labware had been autoclaved before disposal.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We made sure all the biohazardous waste and labware had been autoclaved before disposal.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>* <del class="diffchange diffchange-inline"> </del>Researchers safety</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>* Researchers safety</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We think that good education on safety guidelines and requirements is the primary, yet important and effective way to enable researchers to protect themselves. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We think that good education on safety guidelines and requirements is the primary, yet important and effective way to enable researchers to protect themselves. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>As our first activity this year, we provided all of our new members with basic knowledge and understanding of biotechnology, and then had them trained for their assigned experiments. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>As our first activity this year, we provided all of our new members with basic knowledge and understanding of biotechnology, and then had them trained for their assigned experiments. </div></td></tr>
</table>Tomonorihttp://2010.igem.org/wiki/index.php?title=Team:Kyoto/Safety&diff=200186&oldid=prevTomo: /* 1. Safety of Project */2010-10-27T23:13:29Z<p><span class="autocomment">1. Safety of Project</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* Researchers safety</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* Researchers safety</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We think that good education on safety guidelines and requirements is the primary, yet important and effective way to enable researchers to protect themselves. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We think that good education on safety guidelines and requirements is the primary, yet important and effective way to enable researchers to protect themselves. </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>As our first activity this year, we <del class="diffchange diffchange-inline">first </del>provided all of our new members with basic knowledge and understanding of biotechnology, and then had them trained for their assigned experiments. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>As our first activity this year, we provided all of our new members with basic knowledge and understanding of biotechnology, and then had them trained for their assigned experiments. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Being frequently visited and gone over by senior students and advisors, all of us, including freshmen and sophomores, could carry out all the experiments safely.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Being frequently visited and gone over by senior students and advisors, all of us, including freshmen and sophomores, could carry out all the experiments safely.</div></td></tr>
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</table>Tomohttp://2010.igem.org/wiki/index.php?title=Team:Kyoto/Safety&diff=200047&oldid=prevTomo: /* 1. Safety of Project */2010-10-27T23:08:56Z<p><span class="autocomment">1. Safety of Project</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We made extra sure that hazardous reagents and equipment are handled properly and safely.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We made extra sure that hazardous reagents and equipment are handled properly and safely.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>The major materials we used that may raise safety issues include: <del class="diffchange diffchange-inline"> </del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>The major materials we used that may raise safety issues include: <ins class="diffchange diffchange-inline"> </ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>・Ethidium Bromide (EtBr)---Very commonly used to visualize DNA and RNA because it binds to nucleic acids and fluoresces when exposed to UV. However, its binding property to double-stranded DNA makes itself harmful for organisms at the same time---it is thought to intercalate DNA and deform the molecule, thereby acting as a mutagen. Therefore, care should be taken during its use, storage and disposal. We wore gloves whenever we use EtBr, and all the EtBr contaminated debris were double wrapped and collected separately to be incinerated. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>・Ethidium Bromide (EtBr)---Very commonly used to visualize DNA and RNA because it binds to nucleic acids and fluoresces when exposed to UV. However, its binding property to double-stranded DNA makes itself harmful for organisms at the same time---it is thought to intercalate DNA and deform the molecule, thereby acting as a mutagen. Therefore, care should be taken during its use, storage and disposal. We wore gloves whenever we use EtBr, and all the EtBr contaminated debris were double wrapped and collected separately to be incinerated. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
</table>Tomohttp://2010.igem.org/wiki/index.php?title=Team:Kyoto/Safety&diff=200003&oldid=prevTomo: /* 1. Safety of Project */2010-10-27T23:07:42Z<p><span class="autocomment">1. Safety of Project</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We made extra sure that hazardous reagents and equipment are handled properly and safely.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We made extra sure that hazardous reagents and equipment are handled properly and safely.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>The major materials we used that may raise safety issues <del class="diffchange diffchange-inline">includes</del>: </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>The major materials we used that may raise safety issues <ins class="diffchange diffchange-inline">include</ins>: </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>・Ethidium Bromide (EtBr)---Very commonly used to visualize DNA and RNA because it binds to nucleic acids and fluoresces when exposed to UV. However, its binding property to double-stranded DNA makes itself harmful for organisms at the same time---it is thought to intercalate DNA and deform the molecule, thereby acting as a mutagen. Therefore, care should be taken during its use, storage and disposal. We wore gloves whenever we use EtBr, and all the EtBr contaminated debris were double wrapped and collected separately to be incinerated. </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>・Ethidium Bromide (EtBr)---Very commonly used to visualize DNA and RNA because it binds to nucleic acids and fluoresces when exposed to UV. However, its binding property to double-stranded DNA makes itself harmful for organisms at the same time---it is thought to intercalate DNA and deform the molecule, thereby acting as a mutagen. Therefore, care should be taken during its use, storage and disposal. We wore gloves whenever we use EtBr, and all the EtBr contaminated debris were double wrapped and collected separately to be incinerated. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
</table>Tomohttp://2010.igem.org/wiki/index.php?title=Team:Kyoto/Safety&diff=193857&oldid=prevTomo: /* 4. Other Ideas */2010-10-27T19:41:11Z<p><span class="autocomment">4. Other Ideas</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>In establishing the standard, not only the discussion between researchers and experts in biosafety, but also an interview to participants is necessary. This is because it seems there are big differences in the experimental instruments and how much teams can afford to biosafety. International and national guidelines also have to be concerned.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>In establishing the standard, not only the discussion between researchers and experts in biosafety, but also an interview to participants is necessary. This is because it seems there are big differences in the experimental instruments and how much teams can afford to biosafety. International and national guidelines also have to be concerned.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Once the standards are established, they can be made into an itemized checklist that can be easily referred to even when iGEMers are busy with experiments. Also, in order that these standards are well maintained, iGEM HQ can <del class="diffchange diffchange-inline">visit some teams at random and without appointment</del>.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Once the standards are established, they can be made into an itemized checklist that can be easily referred to even when iGEMers are busy with experiments. Also, in order that these standards are well maintained, iGEM HQ can <ins class="diffchange diffchange-inline">carry out surprise inspections</ins>.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[#top-section|^Top]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[#top-section|^Top]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>----</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>----</div></td></tr>
</table>Tomohttp://2010.igem.org/wiki/index.php?title=Team:Kyoto/Safety&diff=193682&oldid=prevTomo: /* 4. Other Ideas */2010-10-27T19:34:37Z<p><span class="autocomment">4. Other Ideas</span></p>
<table style="background-color: white; color:black;">
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>In establishing the standard, not only the discussion between researchers and experts in biosafety, but also an interview to participants is necessary. This is because it seems there are big differences in the experimental instruments and how much teams can afford to biosafety. International and national guidelines also have to be concerned.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>In establishing the standard, not only the discussion between researchers and experts in biosafety, but also an interview to participants is necessary. This is because it seems there are big differences in the experimental instruments and how much teams can afford to biosafety. International and national guidelines also have to be concerned.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Once the standards are established, they can be made into <del class="diffchange diffchange-inline">a simple </del>checklist <del class="diffchange diffchange-inline">shown by a run of the item </del>that can be easily <del class="diffchange diffchange-inline">refferred </del>even when iGEMers are busy with experiments. Also, in order that these standards are well maintained, iGEM HQ can visit some teams at random and without appointment.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Once the standards are established, they can be made into <ins class="diffchange diffchange-inline">an itemized </ins>checklist that can be easily <ins class="diffchange diffchange-inline">referred to </ins>even when iGEMers are busy with experiments. <ins class="diffchange diffchange-inline"> </ins>Also, in order that these standards are well maintained, iGEM HQ can visit some teams at random and without appointment.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[#top-section|^Top]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[#top-section|^Top]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>----</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>----</div></td></tr>
</table>Tomohttp://2010.igem.org/wiki/index.php?title=Team:Kyoto/Safety&diff=193347&oldid=prevTomo: /* 4. Other Ideas */2010-10-27T19:23:53Z<p><span class="autocomment">4. Other Ideas</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>In establishing the standard, not only the discussion between researchers and experts in biosafety, but also an interview to participants is necessary. This is because it seems there are big differences in the experimental instruments and how much teams can afford to biosafety. International and national guidelines also have to be concerned.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>In establishing the standard, not only the discussion between researchers and experts in biosafety, but also an interview to participants is necessary. This is because it seems there are big differences in the experimental instruments and how much teams can afford to biosafety. International and national guidelines also have to be concerned.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">After </del>the standards are established, they can be made into a simple checklist shown by a run of the item that can be easily refferred even when iGEMers are busy with experiments. Also, in order that these standards are well maintained, iGEM HQ can visit some teams at random and without appointment.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">Once </ins>the standards are established, they can be made into a simple checklist shown by a run of the item that can be easily refferred even when iGEMers are busy with experiments. Also, in order that these standards are well maintained, iGEM HQ can visit some teams at random and without appointment.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[#top-section|^Top]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[#top-section|^Top]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>----</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>----</div></td></tr>
</table>Tomohttp://2010.igem.org/wiki/index.php?title=Team:Kyoto/Safety&diff=193200&oldid=prevTomo: /* 4. Other Ideas */2010-10-27T19:19:00Z<p><span class="autocomment">4. Other Ideas</span></p>
<table style="background-color: white; color:black;">
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>In establishing the standard, not only the discussion between researchers and experts in biosafety, but also an interview to participants is necessary. This is because it seems there are big differences in the experimental instruments and how much teams can afford to biosafety. International and national guidelines also have to be concerned.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>In establishing the standard, not only the discussion between researchers and experts in biosafety, but also an interview to participants is necessary. This is because it seems there are big differences in the experimental instruments and how much teams can afford to biosafety. International and national guidelines also have to be concerned.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>After the standards are established, they can be made into a simple <del class="diffchange diffchange-inline">check list </del>shown by a run of the item that can be easily refferred even when iGEMers are busy with experiments. Also, in order that these standards are well maintained, iGEM HQ can visit some teams at random and without appointment.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>After the standards are established, they can be made into a simple <ins class="diffchange diffchange-inline">checklist </ins>shown by a run of the item that can be easily refferred even when iGEMers are busy with experiments. Also, in order that these standards are well maintained, iGEM HQ can visit some teams at random and without appointment.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[#top-section|^Top]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[#top-section|^Top]]</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>----</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>----</div></td></tr>
</table>Tomohttp://2010.igem.org/wiki/index.php?title=Team:Kyoto/Safety&diff=193001&oldid=prevTomo: /* 1. Safety of Project */2010-10-27T19:12:36Z<p><span class="autocomment">1. Safety of Project</span></p>
<table style="background-color: white; color:black;">
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We made extra sure that hazardous reagents and equipment are handled properly and safely.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>We made extra sure that hazardous reagents and equipment are handled properly and safely.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The major materials we used that may raise safety issues includes: </div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>The major materials we used that may raise safety issues includes: </div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>・Ethidium Bromide (EtBr)---Very commonly used to visualize DNA and RNA because it binds to nucleic acids and fluoresces when exposed to UV. However, its binding property to double-stranded DNA makes itself harmful for organisms at the same time---it is thought to intercalate DNA and deform the molecule, thereby acting as a mutagen. Therefore, care should be taken during its use, storage and disposal. We wore gloves whenever we use EtBr, and all the EtBr contaminated debris were collected separately to be incinerated. </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>・Ethidium Bromide (EtBr)---Very commonly used to visualize DNA and RNA because it binds to nucleic acids and fluoresces when exposed to UV. However, its binding property to double-stranded DNA makes itself harmful for organisms at the same time---it is thought to intercalate DNA and deform the molecule, thereby acting as a mutagen. Therefore, care should be taken during its use, storage and disposal. We wore gloves whenever we use EtBr, and all the EtBr contaminated debris were <ins class="diffchange diffchange-inline">double wrapped and </ins>collected separately to be incinerated. </div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>・Ultra Violet machine---Used in combination with EtBr. As is well known, UV has various hazardous effects on human body. In addition to causing direct and indirect damage to DNA (through subtype UVB and UVC), it also damages collagen fibers, destroys vitamin A, and is especially harmful to eyes and skin. All the more because we are familiar with UV ray, we should be careful when using the machine not to over expose ourselves to it.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>・Ultra Violet machine---Used in combination with EtBr. As is well known, UV has various hazardous effects on human body. In addition to causing direct and indirect damage to DNA (through subtype UVB and UVC), it also damages collagen fibers, destroys vitamin A, and is especially harmful to eyes and skin. All the more because we are familiar with UV ray, we should be careful when using the machine not to over expose ourselves to it.</div></td></tr>
</table>Tomo