Team:Kyoto

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===Abstract===
===Abstract===
<span class="title">'''The Fantastic Lysisbox:'''</span>
<span class="title">'''The Fantastic Lysisbox:'''</span>
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Genetic engineered cell death is imperative for biotechnological usage, such as bioremediation area. For controlling cell death, we designed “Lysisbox” consists of a pair of modules: “Killer gene” and “Anti-killer gene.” As the Killer gene for ''Escherichia coli'', we noted the lysis cassette [SRRz/Rz1 gene] of lambda phage coding for a holin and an endolysin. The holin forms pores in the inner membrane, and the endolysin access to and degrade the peptidoglycan by passing through the pores, leading the E.coli to death. As the Anti-killer gene, we chose S<sub>ΔTMD1</sub> coding for a dominant-negative holin that inhibits the formation of the fatal pores. The balance of these two genes expression level has a key of the E.coli’s life or death. In addition, such controllable membrane pores must show critical functions for all living organisms with lipid membranes. “Lysisbox” will contribute a lot to future projects, thus you must say “FANTASTIC!!!"
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Genetically engineered cell death is essential for the application of biotechnology, such as in bioremediation area. In order to control the cell-death, we designed “Lysisbox”, which consists of a pair of modules: “Killer gene” and “Anti-killer gene.” As the Killer gene for ''Escherichia coli'', we noted the lysis cassette [SRRz/Rz1 gene] of lambda phage coding for holin and endolysin. The holin form pores in the inner membrane and the endolysin access and degrade the peptidoglycan by passing through the pores, leading the E.coli to death. As the Anti-killer gene, we chose S<sub>ΔTMD1</sub> coding for a dominant-negative holin that inhibits the formation of the fatal pores. The balance of these two genes' expression level has the key to the E.coli’s life or death. In addition, such controllable membrane pores must show critical functions for all living organisms with lipid membranes. “Lysisbox” will contribute a lot to future projects and thus you must say “FANTASTIC!!!"
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Revision as of 05:15, 24 October 2010

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Abstract

The Fantastic Lysisbox: Genetically engineered cell death is essential for the application of biotechnology, such as in bioremediation area. In order to control the cell-death, we designed “Lysisbox”, which consists of a pair of modules: “Killer gene” and “Anti-killer gene.” As the Killer gene for Escherichia coli, we noted the lysis cassette [SRRz/Rz1 gene] of lambda phage coding for holin and endolysin. The holin form pores in the inner membrane and the endolysin access and degrade the peptidoglycan by passing through the pores, leading the E.coli to death. As the Anti-killer gene, we chose SΔTMD1 coding for a dominant-negative holin that inhibits the formation of the fatal pores. The balance of these two genes' expression level has the key to the E.coli’s life or death. In addition, such controllable membrane pores must show critical functions for all living organisms with lipid membranes. “Lysisbox” will contribute a lot to future projects and thus you must say “FANTASTIC!!!"

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