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Team:Korea U Seoul/Parts
From 2010.igem.org
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:It detects cadmium, zinc, and arsenic in the media and gives brown, red, and green color. This is our final product to put in a | :It detects cadmium, zinc, and arsenic in the media and gives brown, red, and green color. This is our final product to put in a | ||
:capsule in order to be customized. | :capsule in order to be customized. | ||
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| + | =='''Part:BBa_K400628'''== | ||
| + | *Designed by Seobeom Heo Group: iGEM10_Korea_U_Seoul (2010-10-19) | ||
| + | *mAAA (EcoRI site mutated Aryl Acyl-Amidase) | ||
| + | *Aryl acylamidase is an enzyme that acts in the amide bond between aryl- and acyl- compounds. The most typical reaction is the hydrolysis :of an anilide, producing a carboxylate and aniline, which is reversible. When a p-acetaminophenol was reacted with AAA, the product gave :a purple-red color. As a result, a clone presenting AAA activity from the genomic library gave a color change around the colony when it :was grown on solid media enriched with p-acetaminophenol.Since it gives color to colorless substance, it can be used as a reporter gene to a specific promoter. In our project, we put it :downstream of cadmium-dependent promoter to detect any cadmium present in a solution. | ||
Revision as of 17:19, 27 October 2010
Contents |
Parts
New for iGEM 2010 is the groupparts tag. This tag will generate a table with all of the parts that your team adds to your team sandbox. Note that if you want to document a part you need to document it on the Registry, not on your team wiki.
<groupparts>iGEM010 Korea_U_Seoul</groupparts>
Character
Part:BBa_K400000
- Designed by Jonghwa Kim Group: iGEM10_Korea_U_Seoul (2010-10-21) composite heavy metal-detector(Zn, Cd, As)
- It is a composite part of heavy metal-detecting promoters and reporter genes.
- PyodA-mAAA(BBa_K400628)----PzntA-RFP(BBa_E0040)----ParsR-GFP(BBa_J04450)----
- It detects cadmium, zinc, and arsenic in the media and gives brown, red, and green color. This is our final product to put in a
- capsule in order to be customized.
Part:BBa_K400628
- Designed by Seobeom Heo Group: iGEM10_Korea_U_Seoul (2010-10-19)
- mAAA (EcoRI site mutated Aryl Acyl-Amidase)
- Aryl acylamidase is an enzyme that acts in the amide bond between aryl- and acyl- compounds. The most typical reaction is the hydrolysis :of an anilide, producing a carboxylate and aniline, which is reversible. When a p-acetaminophenol was reacted with AAA, the product gave :a purple-red color. As a result, a clone presenting AAA activity from the genomic library gave a color change around the colony when it :was grown on solid media enriched with p-acetaminophenol.Since it gives color to colorless substance, it can be used as a reporter gene to a specific promoter. In our project, we put it :downstream of cadmium-dependent promoter to detect any cadmium present in a solution.
