Team:KIT-Kyoto/Project

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== The fireworks using AHL as a transmitter. ==
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The bacteria communicate with each other in the same way as people do so using some languages.This was called Quorum Sensing, and we paid our attention to this Quorum Sensing. In Quorum Sensing, the bacteria use the chemical substance called "autoinducer" in substitution for words to say with a human being. The bacteria confirm how much density the same type of bacteria exist by around oneself with autoinducers. When they sense the density is high, the bacteria take action all at once.  
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We produce bioart using Quorum Sensing as materials in a colon bacillus this time. E. coli uses a chemical substance called "N-Acyl-Homoserin-Lacton(AHL)" as autoinducers.
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We thought whether we could reproduce fireworks by bioart using AHL as a communication factor.We use the GFP to express flower firelight.The GPF,which is fluorescence protein discovered by Aequorea victoria,has the property of forming a chromophoric group (chemical structure to develop a color) alone.
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For a rough procedure, fireworks are launched and turned off by the following methods.
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① GPF is expressed in E.coil using AHL as a communication factor
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② Turn off the expressed GPF like a chain reaction.
|[[Image:KIT-Kyoto_logo.png|200px|right|frame]]
|[[Image:KIT-Kyoto_logo.png|200px|right|frame]]
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Revision as of 04:00, 14 July 2010


This is a template page. READ THESE INSTRUCTIONS.
You are provided with this team page template with which to start the iGEM season. You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki. You can find some examples HERE.
You MUST have a team description page, a project abstract, a complete project description, a lab notebook, and a safety page. PLEASE keep all of your pages within your teams namespace.


Contents

The fireworks using AHL as a transmitter.

The bacteria communicate with each other in the same way as people do so using some languages.This was called Quorum Sensing, and we paid our attention to this Quorum Sensing. In Quorum Sensing, the bacteria use the chemical substance called "autoinducer" in substitution for words to say with a human being. The bacteria confirm how much density the same type of bacteria exist by around oneself with autoinducers. When they sense the density is high, the bacteria take action all at once. We produce bioart using Quorum Sensing as materials in a colon bacillus this time. E. coli uses a chemical substance called "N-Acyl-Homoserin-Lacton(AHL)" as autoinducers.

We thought whether we could reproduce fireworks by bioart using AHL as a communication factor.We use the GFP to express flower firelight.The GPF,which is fluorescence protein discovered by Aequorea victoria,has the property of forming a chromophoric group (chemical structure to develop a color) alone.

For a rough procedure, fireworks are launched and turned off by the following methods.

① GPF is expressed in E.coil using AHL as a communication factor

② Turn off the expressed GPF like a chain reaction.

KIT-Kyoto logo.png

Tell us more about your project. Give us background. Use this is the abstract of your project. Be descriptive but concise (1-2 paragraphs)

File:KIT-Kyoto team.png
Your team picture
Team Example


Home Team Official Team Profile Project Parts Submitted to the Registry Modeling Notebook Safety



Overall project

Your abstract




Project Details

Part 2

The Experiments

Part 3

Results