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| Line 18: |
Line 18: |
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| | <br> | | <br> |
| - |
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| - | ==9/6 ~ 9/9 PCR Result==
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| - |
| |
| - | <span style=font-size:12px><b>PCR composition </b></span>
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| - | <table border=1>
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| - | <tr align=center>
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| - | <td><b>Materials</b></td><td><b>Amount</b></td>
| |
| - | </tr>
| |
| - | <tr align=center>
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| - | <td>Template</td><td>100pmole</td>
| |
| - | </tr>
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| - | <tr align=center>
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| - | <td>Primer A</td><td>50pmole</td>
| |
| - | </tr>
| |
| - | <tr align=center>
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| - | <td>Primer B</td><td>50pmole</td>
| |
| - | </tr>
| |
| - | <tr align=center>
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| - | <td>dNTP (dNTP mixture 2.5mM TaKaRa)</td><td>2ul</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td>Taq (Pfu Turbo® DNA Polymerase 2.5U/ μl STRATAGENE)</td><td>0.25ul</td>
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| - | </tr>
| |
| - | <tr align=center>
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| - | <td>10x buffer (10x cloned Pfu Reaction buffer)</td><td>5ul</td>
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| - | </tr>
| |
| - | <tr align=center>
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| - | <td>DW (3rd sterile water)</td><td>rest</td>
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| - | </tr>
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| - | <tr align=center>
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| - | <td>Total</td><td>50ul</td>
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| - | </tr>
| |
| - | </table>
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| - | <br>
| |
| - | <br>
| |
| - | <b>Exp 1</b>
| |
| - | <center>
| |
| - | <table border=1>
| |
| - | <tr align=center>
| |
| - | <td width = "300px"><b>Segment</b></td><td width = "200px"><b>Annealing Temperature</b></td><td width = "300px"><b>Result</b></td>
| |
| - | </tr>
| |
| - | </table>
| |
| - | <table border=1>
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| - | <tr align=center>
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| - | <td width = "40px">a</td><td width = "255px">STAT1</td><td width = "200px">53*C</td><td width = "223px">-</td><td width = "70px">-</td>
| |
| - | </tr>
| |
| - | <tr align=center>
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| - | <td width = "40px">b</td><td width = "255px">V_STAT1</td><td width = "200px">53*C</td><td width = "223px">-</td><td width = "70px">-</td>
| |
| - | </tr>
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| - | <tr align=center>
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| - | <td width = "40px">c</td><td width = "255px">Fusion Antibody receptor</td><td width = "200px">53*C</td><td width = "223px">-</td><td width = "70px">-</td>
| |
| - | </tr>
| |
| - | <tr align=center>
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| - | <td width = "40px">d</td><td width = "255px">Signal peptide</td><td width = "200px">56*C</td><td width = "223px">-</td><td width = "70px">-</td>
| |
| - | </tr>
| |
| - | <tr align=center>
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| - | <td width = "40px">e</td><td width = "255px">Ig-like</td><td width = "200px">56*C</td><td width = "223px">-</td><td width = "70px">-</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">f</td><td width = "255px">Signal Peptide + Ig-like</td><td width = "200px">56*C</td><td width = "223px">-</td><td width = "70px">-</td>
| |
| - | </tr>
| |
| - | <tr align=center>
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| - | <td width = "40px">g</td><td width = "255px">Antibody</td><td width = "200px">56*C</td><td width = "223px">-</td><td width = "70px">-</td>
| |
| - | </tr>
| |
| - | <tr align=center>
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| - | <td width = "40px">h</td><td width = "255px">V_Fusion Antibody Receptor</td><td width = "200px">53*C</td><td width = "223px">-</td><td width = "70px">-</td>
| |
| - | </tr>
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| - | <tr align=center>
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| - | <td width = "40px">i</td><td width = "255px">B_FGFR1</td><td width = "200px">56*C</td><td width = "223px">-</td><td width = "70px">-</td>
| |
| - | </tr>
| |
| - | <tr align=center>
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| - | <td width = "40px">j</td><td width = "255px">VB_FGFR1</td><td width = "200px">56*C</td><td width = "223px">-</td><td width = "70px">-</td>
| |
| - | </tr>
| |
| - | </table>
| |
| - | <br>
| |
| - | [[Image:KAIST-exp1.png|560px]]
| |
| - | </center>
| |
| - | <br>
| |
| - | <br>
| |
| - | <b>Exp 2</b>
| |
| - | <center>
| |
| - | <table border=1>
| |
| - | <tr align=center>
| |
| - | <td width = "300px"><b>Segment</b></td><td width = "200px"><b>Annealing Temperature</b></td><td width = "300px"><b>Result</b></td>
| |
| - | </tr>
| |
| - | </table>
| |
| - | <table border=1>
| |
| - | <tr align=center>
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| - | <td width = "40px">a</td><td width = "255px">STAT1</td><td width = "200px">48*C</td><td width = "223px">60%</td><td width = "70px">2139bp</td>
| |
| - | </tr>
| |
| - | <tr align=center>
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| - | <td width = "40px">b</td><td width = "255px">V_STAT1</td><td width = "200px">48*C</td><td width = "223px">-</td><td width = "70px">-</td>
| |
| - | </tr>
| |
| - | </table>
| |
| - | <br>
| |
| - | [[Image:KAIST-exp2.png|260px]]
| |
| - | </center>
| |
| - | <br>
| |
| - | <br>
| |
| - | <b>Exp 3</b>
| |
| - | <center>
| |
| - | <table border=1>
| |
| - | <tr align=center>
| |
| - | <td width = "300px"><b>Segment</b></td><td width = "200px"><b>Annealing Temperature</b></td><td width = "300px"><b>Result</b></td>
| |
| - | </tr>
| |
| - | </table>
| |
| - | <table border=1>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">a</td><td width = "255px"><font color = red>STAT1</font></td><td width = "200px">50*C</td><td width = "223px">100%</td><td width = "70px">2139bp</td>
| |
| - | </tr>
| |
| - | <tr align=center>
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| - | <td width = "40px">b</td><td width = "255px">V_STAT1</td><td width = "200px">50*C</td><td width = "223px">-</td><td width = "70px">-</td>
| |
| - | </tr>
| |
| - | </table>
| |
| - | <br>
| |
| - | [[Image:KAIST-exp3.png|260px]]
| |
| - | </center>
| |
| - | <br>
| |
| - | <br>
| |
| - | <b>Exp 4</b>
| |
| - | <center>
| |
| - | <table border=1>
| |
| - | <tr align=center>
| |
| - | <td width = "300px"><b>Segment</b></td><td width = "200px"><b>Annealing Temperature</b></td><td width = "300px"><b>Result</b></td>
| |
| - | </tr>
| |
| - | </table>
| |
| - | <table border=1>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">a</td><td width = "255px">Fusion Antibody receptor</td><td width = "200px">50*C</td><td width = "223px">-</td><td width = "70px">-</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">b</td><td width = "255px">Signal peptide</td><td width = "200px">50*C</td><td width = "223px">-</td><td width = "70px">-</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">c</td><td width = "255px">Ig-like</td><td width = "200px">50*C</td><td width = "223px">60%</td><td width = "70px">339bp</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">d</td><td width = "255px">Signal Peptide + Ig-like</td><td width = "200px">50*C</td><td width = "223px">-</td><td width = "70px">-</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">e</td><td width = "255px">Antibody</td><td width = "200px">50*C</td><td width = "223px">-</td><td width = "70px">-</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">f</td><td width = "255px">V_Fusion Antibody Receptor</td><td width = "200px">50*C</td><td width = "223px">-</td><td width = "70px">-</td>
| |
| - | </tr>
| |
| - | </table>
| |
| - | <br>
| |
| - | [[Image:KAIST-exp4.png|300px]]
| |
| - | </center>
| |
| - | <br>
| |
| - | <br>
| |
| - |
| |
| - | ==9/10 ~ 9/12 PCR Result==
| |
| - |
| |
| - | <span style=font-size:12px><b>PCR composition </b></span>
| |
| - | <table border=1>
| |
| - | <tr align=center>
| |
| - | <td><b>Materials</b></td><td><b>Amount</b></td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td>Template</td><td>150pmole</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td>Primer A</td><td>50pmole</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td>Primer B</td><td>50pmole</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td>dNTP (dNTP mixture 2.5mM TaKaRa)</td><td>5ul</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td>Taq (Pfu Turbo® DNA Polymerase 2.5U/ μl STRATAGENE)</td><td>0.25ul</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td>10x buffer (10x cloned Pfu Reaction buffer)</td><td>5ul</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td>DW (3rd sterile water)</td><td>rest</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td>Total</td><td>50ul</td>
| |
| - | </tr>
| |
| - | </table>
| |
| - | <br>
| |
| - | <br>
| |
| - | <b>Exp 1</b>
| |
| - | <center>
| |
| - | <table border=1>
| |
| - | <tr align=center>
| |
| - | <td width = "300px"><b>Segment</b></td><td width = "200px"><b>Annealing Temperature</b></td><td width = "300px"><b>Result</b></td>
| |
| - | </tr>
| |
| - | </table>
| |
| - | <table border=1>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">a</td><td width = "255px">STAT1</td><td width = "200px">60*C</td><td width = "223px">-</td><td width = "70px">-</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">b</td><td width = "255px">V_STAT1</td><td width = "200px">60*C</td><td width = "223px">-</td><td width = "70px">-</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">c</td><td width = "255px">Fusion Antibody receptor</td><td width = "200px">60*C</td><td width = "223px">70%</td><td width = "70px">1095bp</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">d</td><td width = "255px">Signal peptide</td><td width = "200px">60*C</td><td width = "223px">-</td><td width = "70px">-</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">e</td><td width = "255px">Ig-like</td><td width = "200px">60*C</td><td width = "223px">60%</td><td width = "70px">339bp</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">f</td><td width = "255px">Signal Peptide + Ig-like</td><td width = "200px">60*C</td><td width = "223px">-</td><td width = "70px">-</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">g</td><td width = "255px"><font color = red>Antibody</font></td><td width = "200px">60*C</td><td width = "223px">100%</td><td width = "70px">669bp</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">h</td><td width = "255px">V_Fusion Antibody Receptor</td><td width = "200px">60*C</td><td width = "223px">-</td><td width = "70px">-</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">i</td><td width = "255px">B_FGFR1</td><td width = "200px">60*C</td><td width = "223px">-</td><td width = "70px">-</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">j</td><td width = "255px">VB_FGFR1</td><td width = "200px">60*C</td><td width = "223px">-</td><td width = "70px">-</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">x1</td><td width = "255px">negative 1</td><td width = "200px">60*C</td><td width = "223px">-</td><td width = "70px">-</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">x2</td><td width = "255px">negative 2</td><td width = "200px">60*C</td><td width = "223px">-</td><td width = "70px">-</td>
| |
| - | </tr>
| |
| - | </table>
| |
| - | <br>
| |
| - | [[Image:KAIST-exp5.png|700px]]
| |
| - | </center>
| |
| - | <br>
| |
| - | <br>
| |
| - | <b>Exp 2</b>
| |
| - | <center>
| |
| - | <table border=1>
| |
| - | <tr align=center>
| |
| - | <td width = "300px"><b>Segment</b></td><td width = "200px"><b>Annealing Temperature</b></td><td width = "300px"><b>Result</b></td>
| |
| - | </tr>
| |
| - | </table>
| |
| - | <table border=1>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">a</td><td width = "255px">STAT1</td><td width = "200px">58*C</td><td width = "223px">60%</td><td width = "70px">2139bp</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">b</td><td width = "255px">V_STAT1</td><td width = "200px">58*C</td><td width = "223px">-</td><td width = "70px">-</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">c</td><td width = "255px">Fusion Antibody receptor</td><td width = "200px">58*C</td><td width = "223px">80%</td><td width = "70px">1095bp</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">d</td><td width = "255px">Signal peptide</td><td width = "200px">58*C</td><td width = "223px">80%</td><td width = "70px">87bp</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">e</td><td width = "255px"><font color = red>Ig-like</font></td><td width = "200px">58*C</td><td width = "223px">100%</td><td width = "70px">339bp</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">f</td><td width = "255px">Signal Peptide + Ig-like</td><td width = "200px">58*C</td><td width = "223px">80%</td><td width = "70px">426bp</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">g</td><td width = "255px"><font color = red>Antibody</font></td><td width = "200px">58*C</td><td width = "223px">100%</td><td width = "70px">669bp</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">h</td><td width = "255px">V_Fusion Antibody Receptor</td><td width = "200px">58*C</td><td width = "223px">-</td><td width = "70px">-</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">i</td><td width = "255px">B_FGFR1</td><td width = "200px">58*C</td><td width = "223px">-</td><td width = "70px">-</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">j</td><td width = "255px">VB_FGFR1</td><td width = "200px">58*C</td><td width = "223px">-</td><td width = "70px">-</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">x</td><td width = "255px">negative</td><td width = "200px">58*C</td><td width = "223px">-</td><td width = "70px">-</td>
| |
| - | </tr>
| |
| - | </table>
| |
| - | <br>
| |
| - | [[Image:KAIST-exp6.png|700px]]
| |
| - | </center>
| |
| - | <br>
| |
| - | <br>
| |
| - | <b>Exp 3</b>
| |
| - | <center>
| |
| - | <table border=1>
| |
| - | <tr align=center>
| |
| - | <td width = "300px"><b>Segment</b></td><td width = "200px"><b>Annealing Temperature</b></td><td width = "300px"><b>Result</b></td>
| |
| - | </tr>
| |
| - | </table>
| |
| - | <table border=1>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">a</td><td width = "255px">STAT1</td><td width = "200px">62*C</td><td width = "223px">-</td><td width = "70px">-</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">b</td><td width = "255px">V_STAT1</td><td width = "200px">62*C</td><td width = "223px">-</td><td width = "70px">-</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">c</td><td width = "255px">Fusion Antibody receptor</td><td width = "200px">62*C</td><td width = "223px">10%</td><td width = "70px">1095bp</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">d</td><td width = "255px">Signal peptide</td><td width = "200px">62*C</td><td width = "223px">-</td><td width = "70px">-</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">e</td><td width = "255px"><font color = red>Ig-like</font></td><td width = "200px">62*C</td><td width = "223px">-</td><td width = "70px">-</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">f</td><td width = "255px">Signal Peptide + Ig-like</td><td width = "200px">62*C</td><td width = "223px">-</td><td width = "70px">-</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">g</td><td width = "255px">Antibody<td width = "200px">62*C</td><td width = "223px">-</td><td width = "70px">-</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">h</td><td width = "255px">V_Fusion Antibody Receptor</td><td width = "200px">62*C</td><td width = "223px">-</td><td width = "70px">-</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">i</td><td width = "255px">B_FGFR1</td><td width = "200px">62*C</td><td width = "223px">-</td><td width = "70px">-</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">j</td><td width = "255px">VB_FGFR1</td><td width = "200px">62*C</td><td width = "223px">-</td><td width = "70px">-</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">x</td><td width = "255px">negative</td><td width = "200px">62*C</td><td width = "223px">-</td><td width = "70px">-</td>
| |
| - | </tr>
| |
| - | </table>
| |
| - | <br>
| |
| - | [[Image:KAIST-exp7.png|640px]]
| |
| - | </center>
| |
| - | <br>
| |
| - | <br>
| |
| - | <b>Exp 4</b>
| |
| - | <center>
| |
| - | <table border=1>
| |
| - | <tr align=center>
| |
| - | <td width = "300px"><b>Segment</b></td><td width = "200px"><b>Annealing Temperature</b></td><td width = "300px"><b>Result</b></td>
| |
| - | </tr>
| |
| - | </table>
| |
| - | <table border=1>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">a</td><td width = "255px">STAT1</td><td width = "200px">56*C</td><td width = "223px">-</td><td width = "70px">-</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">b</td><td width = "255px">V_STAT1</td><td width = "200px">56*C</td><td width = "223px">-</td><td width = "70px">-</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">c</td><td width = "255px">Fusion Antibody receptor</td><td width = "200px">56*C</td><td width = "223px">5%</td><td width = "70px">1095bp</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">d</td><td width = "255px">Signal peptide</td><td width = "200px">56*C</td><td width = "223px">-</td><td width = "70px">-</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">e</td><td width = "255px">Ig-like</td><td width = "200px">56*C</td><td width = "223px">80%</td><td width = "70px">339bp</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">f</td><td width = "255px">Signal Peptide + Ig-like</td><td width = "200px">56*C</td><td width = "223px">-</td><td width = "70px">-</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">g</td><td width = "255px">Antibody</td><td width = "200px">56*C</td><td width = "223px">80%</td><td width = "70px">669bp</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">h</td><td width = "255px">V_Fusion Antibody Receptor</td><td width = "200px">56*C</td><td width = "223px">-</td><td width = "70px">-</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">i</td><td width = "255px">B_FGFR1</td><td width = "200px">56*C</td><td width = "223px">-</td><td width = "70px">-</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">j</td><td width = "255px">VB_FGFR1</td><td width = "200px">56*C</td><td width = "223px">-</td><td width = "70px">-</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">x</td><td width = "255px">negative</td><td width = "200px">56*C</td><td width = "223px">-</td><td width = "70px">-</td>
| |
| - | </tr>
| |
| - | </table>
| |
| - | <br>
| |
| - | [[Image:KAIST-exp8.png|640px]]
| |
| - | </center>
| |
| - | <br>
| |
| - | <br>
| |
| - | <b>Exp 5</b>
| |
| - | <center>
| |
| - | <table border=1>
| |
| - | <tr align=center>
| |
| - | <td width = "300px"><b>Segment</b></td><td width = "200px"><b>Annealing Temperature</b></td><td width = "300px"><b>Result</b></td>
| |
| - | </tr>
| |
| - | </table>
| |
| - | <table border=1>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">a</td><td width = "255px">V_STAT1</td><td width = "200px">64*C</td><td width = "223px">-</td><td width = "70px">-</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">b</td><td width = "255px">B_FGFR1</td><td width = "200px">64*C</td><td width = "223px">5%</td><td width = "70px">1398bp</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">c</td><td width = "255px">VB_FGFR1</td><td width = "200px">64*C</td><td width = "223px">-</td><td width = "70px">-</td>
| |
| - | </tr>
| |
| - | </table>
| |
| - | <br>
| |
| - | [[Image:KAIST-exp9.png|320px]]
| |
| - | </center>
| |
| - | <br>
| |
| - | <br>
| |
| - | <b>Exp 6</b>
| |
| - | <center>
| |
| - | <table border=1>
| |
| - | <tr align=center>
| |
| - | <td width = "300px"><b>Segment</b></td><td width = "200px"><b>Annealing Temperature</b></td><td width = "300px"><b>Result</b></td>
| |
| - | </tr>
| |
| - | </table>
| |
| - | <table border=1>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">a</td><td width = "255px">V_STAT1</td><td width = "200px">64*C</td><td width = "223px">-</td><td width = "70px">-</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">b</td><td width = "255px">B_FGFR1</td><td width = "200px">64*C</td><td width = "223px">3%</td><td width = "70px">1398bp</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">c</td><td width = "255px">VB_FGFR1</td><td width = "200px">64*C</td><td width = "223px">-</td><td width = "70px">-</td>
| |
| - | </tr>
| |
| - | </table>
| |
| - | <br>
| |
| - | [[Image:KAIST-exp10.png|320px]]
| |
| - | </center>
| |
| - | <br>
| |
| - | <br>
| |
| - | <b>Exp 7</b>
| |
| - | <center>
| |
| - | <table border=1>
| |
| - | <tr align=center>
| |
| - | <td width = "300px"><b>Segment</b></td><td width = "200px"><b>Annealing Temperature</b></td><td width = "300px"><b>Result</b></td>
| |
| - | </tr>
| |
| - | </table>
| |
| - | <table border=1>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">a</td><td width = "255px">V_STAT1</td><td width = "200px">68*C</td><td width = "223px">-</td><td width = "70px">-</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">b</td><td width = "255px">B_FGFR1</td><td width = "200px">68*C</td><td width = "223px">10%</td><td width = "70px">1398bp</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">c</td><td width = "255px">VB_FGFR1</td><td width = "200px">68*C</td><td width = "223px">-</td><td width = "70px">-</td>
| |
| - | </tr>
| |
| - | </table>
| |
| - | <br>
| |
| - | [[Image:KAIST-exp12.png|400px]]
| |
| - | </center>
| |
| - | <br>
| |
| - | <br>
| |
| - | <b>Exp 8</b>
| |
| - | <center>
| |
| - | <table border=1>
| |
| - | <tr align=center>
| |
| - | <td width = "300px"><b>Segment</b></td><td width = "200px"><b>Annealing Temperature</b></td><td width = "300px"><b>Result</b></td>
| |
| - | </tr>
| |
| - | </table>
| |
| - | <table border=1>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">a</td><td width = "255px">V_STAT1</td><td width = "200px">70*C</td><td width = "223px">-</td><td width = "70px">-</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">b</td><td width = "255px">B_FGFR1</td><td width = "200px">70*C</td><td width = "223px">3%</td><td width = "70px">1398bp</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">c</td><td width = "255px">VB_FGFR1</td><td width = "200px">70*C</td><td width = "223px">-</td><td width = "70px">-</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">c</td><td width = "255px">5'UTR</td><td width = "200px">70*C</td><td width = "223px">-</td><td width = "70px">-</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">c</td><td width = "255px">3'UTR</td><td width = "200px">70*C</td><td width = "223px">-</td><td width = "70px">-</td>
| |
| - | </tr>
| |
| - | </table>
| |
| - | <br>
| |
| - | [[Image:KAIST-exp13.png|400px]]
| |
| - | </center>
| |
| - | <br>
| |
| - | <br>
| |
| - | <b>Exp 9</b>
| |
| - | <center>
| |
| - | <table border=1>
| |
| - | <tr align=center>
| |
| - | <td width = "300px"><b>Segment</b></td><td width = "200px"><b>Annealing Temperature</b></td><td width = "300px"><b>Result</b></td>
| |
| - | </tr>
| |
| - | </table>
| |
| - | <table border=1>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">a</td><td width = "255px">V_STAT1</td><td width = "200px">67*C</td><td width = "223px">-</td><td width = "70px">-</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">b</td><td width = "255px">B_FGFR1</td><td width = "200px">67*C</td><td width = "223px">6%</td><td width = "70px">1398bp</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">c</td><td width = "255px">VB_FGFR1</td><td width = "200px">67*C</td><td width = "223px">-</td><td width = "70px">-</td>
| |
| - | </tr>
| |
| - | </table>
| |
| - | <br>
| |
| - | [[Image:KAIST-exp14.png|320px]]
| |
| - | </center>
| |
| - | <br>
| |
| - | <br>
| |
| - |
| |
| - | ==9/13 ~ 9/16 PCR Result==
| |
| - |
| |
| - | <span style=font-size:12px><b>PCR composition </b></span>
| |
| - | <table border=1>
| |
| - | <tr align=center>
| |
| - | <td><b>Materials</b></td><td><b>Amount</b></td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td>Template</td><td>100pmole</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td>Primer A</td><td>50pmole</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td>Primer B</td><td>50pmole</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td>Bioneer Accupower HF PCR premix </td><td> < 1ul</td>
| |
| - | </tr>
| |
| - | <tr align=center><td>DW (3rd sterile water)</td><td>rest</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td>Total</td><td>50ul</td>
| |
| - | </tr>
| |
| - | </table>
| |
| - | <br>
| |
| - | <br>
| |
| - | <b>Exp 1</b>
| |
| - | <center>
| |
| - | <table border=1>
| |
| - | <tr align=center>
| |
| - | <td width = "300px"><b>Segment</b></td><td width = "200px"><b>Annealing Temperature</b></td><td width = "300px"><b>Result</b></td>
| |
| - | </tr>
| |
| - | </table>
| |
| - | <table border=1>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">a</td><td width = "255px"><font color=red>STAT1</font></td><td width = "200px">50*C</td><td width = "223px">100%</td><td width = "70px">2139bp</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">b</td><td width = "255px"><font color=red>STAT1</font></td><td width = "200px">52*C</td><td width = "223px">100%</td><td width = "70px">2139bp</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">c</td><td width = "255px"><font color=red>STAT1</font></td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">2139bp</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">d</td><td width = "255px"><font color=red>STAT1</font></td><td width = "200px">56*C</td><td width = "223px">100%</td><td width = "70px">2139bp</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">e</td><td width = "255px"><font color=red>STAT1</font></td><td width = "200px">58*C</td><td width = "223px">100%</td><td width = "70px">2139bp</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">f</td><td width = "255px"><font color=red>STAT1</font></td><td width = "200px">60*C</td><td width = "223px">100%</td><td width = "70px">2139bp</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">g</td><td width = "255px"><font color=red>V_STAT1</font></td><td width = "200px">50*C</td><td width = "223px">100%</td><td width = "70px">2151bp</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">h</td><td width = "255px"><font color=red>V_STAT1</font></td><td width = "200px">52*C</td><td width = "223px">100%</td><td width = "70px">2151bp</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">i</td><td width = "255px"><font color=red>V_STAT1</font></td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">2151bp</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">j</td><td width = "255px"><font color=red>V_STAT1</font></td><td width = "200px">56*C</td><td width = "223px">100%</td><td width = "70px">2151bp</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">k</td><td width = "255px"><font color=red>V_STAT1</font></td><td width = "200px">58*C</td><td width = "223px">100%</td><td width = "70px">2151bp</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">l</td><td width = "255px"><font color=red>V_STAT1</font></td><td width = "200px">60*C</td><td width = "223px">100%</td><td width = "70px">2151bp</td>
| |
| - | </tr>
| |
| - | </table>
| |
| - | <br>
| |
| - | [[Image:KAIST-exp21.png|700px]]
| |
| - | </center>
| |
| - | <br>
| |
| - | <br>
| |
| - | <b>Exp 2</b>
| |
| - | <center>
| |
| - | <table border=1>
| |
| - | <tr align=center>
| |
| - | <td width = "300px"><b>Segment</b></td><td width = "200px"><b>Annealing Temperature</b></td><td width = "300px"><b>Result</b></td>
| |
| - | </tr>
| |
| - | </table>
| |
| - | <table border=1>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">a</td><td width = "255px"><font color=red>B_FGFR</font></td><td width = "200px">50*C</td><td width = "223px">100%</td><td width = "70px">1398bp</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">b</td><td width = "255px"><font color=red>B_FGFR</font></td><td width = "200px">52*C</td><td width = "223px">100%</td><td width = "70px">1398bp</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">c</td><td width = "255px"><font color=red>B_FGFR</font></td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">1398bp</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">d</td><td width = "255px"><font color=red>B_FGFR</font></td><td width = "200px">56*C</td><td width = "223px">100%</td><td width = "70px">1398bp</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">e</td><td width = "255px"><font color=red>B_FGFR</font></td><td width = "200px">58*C</td><td width = "223px">100%</td><td width = "70px">1398bp</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">f</td><td width = "255px"><font color=red>B_FGFR</font></td><td width = "200px">60*C</td><td width = "223px">100%</td><td width = "70px">1398bp</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">g</td><td width = "255px"><font color=red>VB_FGFR</font></td><td width = "200px">50*C</td><td width = "223px">100%</td><td width = "70px">1420bp</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">h</td><td width = "255px"><font color=red>VB_FGFR</font></td><td width = "200px">52*C</td><td width = "223px">100%</td><td width = "70px">1420bp</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">i</td><td width = "255px"><font color=red>VB_FGFR</font></td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">1420bp</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">j</td><td width = "255px"><font color=red>VB_FGFR</font></td><td width = "200px">56*C</td><td width = "223px">100%</td><td width = "70px">1420bp</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">k</td><td width = "255px"><font color=red>VB_FGFR</font></td><td width = "200px">58*C</td><td width = "223px">100%</td><td width = "70px">1420bp</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">l</td><td width = "255px"><font color=red>VB_FGFR</font></td><td width = "200px">60*C</td><td width = "223px">100%</td><td width = "70px">1420bp</td>
| |
| - | </tr>
| |
| - | </table>
| |
| - | <br>
| |
| - | [[Image:KAIST-exp22.png|700px]]
| |
| - | </center>
| |
| - | <br>
| |
| - | <br>
| |
| - | <b>Exp 3</b>
| |
| - | <center>
| |
| - | <table border=1>
| |
| - | <tr align=center>
| |
| - | <td width = "300px"><b>Segment</b></td><td width = "200px"><b>Annealing Temperature</b></td><td width = "300px"><b>Result</b></td>
| |
| - | </tr>
| |
| - | </table>
| |
| - | <table border=1>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">a</td><td width = "255px">Signal peptide</td><td width = "200px">61*C</td><td width = "223px">30%</td><td width = "70px">87bp</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">b</td><td width = "255px"><font color=red>Ig-like</font></td><td width = "200px">61*C</td><td width = "223px">100%</td><td width = "70px">339bp</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">c</td><td width = "255px"><font color=red>Signal peptide + Ig-like</font></td><td width = "200px">61*C</td><td width = "223px">100%</td><td width = "70px">426bp</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">d</td><td width = "255px">Signal peptide</td><td width = "200px">59*C</td><td width = "223px">30%</td><td width = "70px">87bp</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">e</td><td width = "255px"><font color=red>Ig-like</font></td><td width = "200px">59*C</td><td width = "223px">100%</td><td width = "70px">339bp</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">f</td><td width = "255px"><font color=red>Signal peptide + Ig-like</font></td><td width = "200px">59*C</td><td width = "223px">100%</td><td width = "70px">426bp</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">g</td><td width = "255px">3'UTR</td><td width = "200px">52*C</td><td width = "223px">50%</td><td width = "70px">444bp</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">h</td><td width = "255px">3'UTR</td><td width = "200px">54*C</td><td width = "223px">50%</td><td width = "70px">444bp</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">i</td><td width = "255px"><font color=red>3'UTR</font></td><td width = "200px">56*C</td><td width = "223px">100%</td><td width = "70px">444bp</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">j</td><td width = "255px"><font color=red>3'UTR</font></td><td width = "200px">58*C</td><td width = "223px">100%</td><td width = "70px">444bp</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">k</td><td width = "255px"><font color=red>3'UTR</font></td><td width = "200px">60*C</td><td width = "223px">100%</td><td width = "70px">444bp</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">l</td><td width = "255px"><font color=red>5'UTR</font></td><td width = "200px">52*C</td><td width = "223px">100%</td><td width = "70px">500bp</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">m</td><td width = "255px"><font color=red>5'UTR</font></td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">500bp</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">n</td><td width = "255px"><font color=red>5'UTR</font></td><td width = "200px">56*C</td><td width = "223px">100%</td><td width = "70px">500bp</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">o</td><td width = "255px"><font color=red>5'UTR</font></td><td width = "200px">58*C</td><td width = "223px">100%</td><td width = "70px">500bp</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">p</td><td width = "255px">5'UTR</td><td width = "200px">69*C</td><td width = "223px">70%</td><td width = "70px">500bp</td>
| |
| - | </tr>
| |
| - | </table>
| |
| - | <br>
| |
| - | [[Image:KAIST-exp23.png|700px]]
| |
| - | </center>
| |
| - | <br>
| |
| - | <br>
| |
| - | <b>Exp 4</b>
| |
| - | <center>
| |
| - | <table border=1>
| |
| - | <tr align=center>
| |
| - | <td width = "300px"><b>Segment</b></td><td width = "200px"><b>Annealing Temperature</b></td><td width = "300px"><b>Result</b></td>
| |
| - | </tr>
| |
| - | </table>
| |
| - | <table border=1>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">a</td><td width = "255px"><font color=red>F_Fusion antibody receptor</font></td><td width = "200px">60*C</td><td width = "223px">100%</td><td width = "70px">1095bp</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">b</td><td width = "255px"><font color=red>F_Fusion antibody receptor</font></td><td width = "200px">58*C</td><td width = "223px">100%</td><td width = "70px">1095bp</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">c</td><td width = "255px"><font color=red>Antibody</font></td><td width = "200px">60*C</td><td width = "223px">100%</td><td width = "70px">669bp</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">d</td><td width = "255px"><font color=red>Antibody</font></td><td width = "200px">58*C</td><td width = "223px">100%</td><td width = "70px">669bp</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">e</td><td width = "255px"><font color=red>VF_Fusion antibody receptor</font></td><td width = "200px">60*C</td><td width = "223px">100%</td><td width = "70px">1107bp</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">f</td><td width = "255px"><font color=red>VF_Fusion antibody receptor</font></td><td width = "200px">58*C</td><td width = "223px">100%</td><td width = "70px">1107bp</td>
| |
| - | </tr>
| |
| - |
| |
| - | </table>
| |
| - | <br>
| |
| - | [[Image:KAIST-exp24.png|360px]]
| |
| - | </center>
| |
| - | <br>
| |
| - | <br>
| |
| - |
| |
| - | == 9/17 PCR result ==
| |
| - |
| |
| - | <span style=font-size:12px><b>PCR composition </b></span>
| |
| - | <table border=1>
| |
| - | <tr align=center>
| |
| - | <td><b>Materials</b></td><td><b>Amount</b></td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td>Template</td><td>100pmole</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td>Primer A</td><td>50pmole</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td>Primer B</td><td>50pmole</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td>Bioneer Accupower HF PCR premix </td><td> < 1ul</td>
| |
| - | </tr>
| |
| - | <tr align=center><td>DW (3rd sterile water)</td><td>rest</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td>Total</td><td>50ul</td>
| |
| - | </tr>
| |
| - | </table>
| |
| - | <br>
| |
| - | <br>
| |
| - | <b>Exp 1</b>
| |
| - | <center>
| |
| - | <table border=1>
| |
| - | <tr align=center>
| |
| - | <td width = "300px"><b>Segment</b></td><td width = "200px"><b>Annealing Temperature</b></td><td width = "300px"><b>Result</b></td>
| |
| - | </tr>
| |
| - | </table>
| |
| - | <table border=1>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">a</td><td width = "255px">Selection marker(41)</td><td width = "200px">50*C</td><td width = "223px">0%</td><td width = "70px">--</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">b</td><td width = "255px">Selection marker(41)</td><td width = "200px">52*C</td><td width = "223px">0%</td><td width = "70px">--</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">c</td><td width = "255px">Selection marker(41)</td><td width = "200px">54*C</td><td width = "223px">0%</td><td width = "70px">--</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">d</td><td width = "255px">Selection marker(41)</td><td width = "200px">56*C</td><td width = "223px">0%</td><td width = "70px">--</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">e</td><td width = "255px">Selection marker(42)</td><td width = "200px">50*C</td><td width = "223px">0%</td><td width = "70px">--</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">f</td><td width = "255px">Selection marker(42)</td><td width = "200px">52*C</td><td width = "223px">0%</td><td width = "70px">--</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">g</td><td width = "255px">Selection marker(42)</td><td width = "200px">54*C</td><td width = "223px">0%</td><td width = "70px">--</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">h</td><td width = "255px">Selection marker(42)</td><td width = "200px">56*C</td><td width = "223px">0%</td><td width = "70px">--</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">i</td><td width = "255px">F_Fusion antibody receptor</td><td width = "200px">50*C</td><td width = "223px">50%</td><td width = "70px">1107bp</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">j</td><td width = "255px">F_Fusion antibody receptor</td><td width = "200px">52*C</td><td width = "223px">80%</td><td width = "70px">1107bp</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">k</td><td width = "255px"><font color=red>B_Fusion antibody receptor</font></td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">1398bp</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">l</td><td width = "255px"><font color=red>B_Fusion antibody receptor</font></td><td width = "200px">56*C</td><td width = "223px">100%</td><td width = "70px">1398bp</td>
| |
| - | </tr>
| |
| - | </table>
| |
| - | <br>
| |
| - | [[Image:KAIST-exp25.png|360px]]
| |
| - | </center>
| |
| - | <br>
| |
| - | <br>
| |
| - | <b>Exp 2</b>
| |
| - | <center>
| |
| - | <table border=1>
| |
| - | <tr align=center>
| |
| - | <td width = "300px"><b>Segment</b></td><td width = "200px"><b>Annealing Temperature</b></td><td width = "300px"><b>Result</b></td>
| |
| - | </tr>
| |
| - | </table>
| |
| - | <table border=1>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">a</td><td width = "255px"><font color=red>HA1</font></td><td width = "200px">50*C</td><td width = "223px">100%</td><td width = "70px">87bp</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">b</td><td width = "255px"><font color=red>HA1</font></td><td width = "200px">52*C</td><td width = "223px">100%</td><td width = "70px">87bp</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">c</td><td width = "255px"><font color=red>HA1</font></td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">87bp</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">d</td><td width = "255px"><font color=red>HA1</font></td><td width = "200px">56*C</td><td width = "223px">100%</td><td width = "70px">87bp</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">e</td><td width = "255px"><font color=red>HA2</font></td><td width = "200px">50*C</td><td width = "223px">100%</td><td width = "70px">87bp</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">f</td><td width = "255px"><font color=red>HA2</font></td><td width = "200px">52*C</td><td width = "223px">100%</td><td width = "70px">87bp</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">g</td><td width = "255px"><font color=red>HA2</font></td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">87bp</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">h</td><td width = "255px"><font color=red>HA2</font></td><td width = "200px">56*C</td><td width = "223px">100%</td><td width = "70px">87bp</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">i</td><td width = "255px"><font color=red>Proximal promoter</font></td><td width = "200px">50*C</td><td width = "223px">100%</td><td width = "70px">120bp</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">j</td><td width = "255px"><font color=red>Proximal promoter</font></td><td width = "200px">52*C</td><td width = "223px">100%</td><td width = "70px">120bp</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">k</td><td width = "255px"><font color=red>Proximal promoter</font></td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">120bp</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">l</td><td width = "255px"><font color=red>Proximal promoter</font></td><td width = "200px">56*C</td><td width = "223px">100%</td><td width = "70px">120bp</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">m</td><td width = "255px"><font color=red>5' UTR</font></td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">500bp</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">n</td><td width = "255px"><font color=red>5' UTR</font></td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">500bp</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">o</td><td width = "255px"><font color=red>3' UTR</font></td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">444bp</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">p</td><td width = "255px"><font color=red>3' UTR</font></td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">444bp</td>
| |
| - | </tr>
| |
| - | </table>
| |
| - | <br>
| |
| - | [[Image:KAIST-exp26.png|360px]]
| |
| - | </center>
| |
| - | <br>
| |
| - | <br>
| |
| - |
| |
| - | == 9/18 PCR result ==
| |
| - |
| |
| - | <span style=font-size:12px><b>PCR composition </b></span>
| |
| - | <table border=1>
| |
| - | <tr align=center>
| |
| - | <td><b>Materials</b></td><td><b>Amount</b></td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td>Template</td><td>100pmole</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td>Primer A</td><td>50pmole</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td>Primer B</td><td>50pmole</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td>Bioneer Accupower HF PCR premix </td><td> < 1ul</td>
| |
| - | </tr>
| |
| - | <tr align=center><td>DW (3rd sterile water)</td><td>rest</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td>Total</td><td>50ul</td>
| |
| - | </tr>
| |
| - | </table>
| |
| - | <br>
| |
| - | <br>
| |
| - | <b>Exp 1</b>
| |
| - | <center>
| |
| - | <table border=1>
| |
| - | <tr align=center>
| |
| - | <td width = "300px"><b>Segment</b></td><td width = "200px"><b>Annealing Temperature</b></td><td width = "300px"><b>Result</b></td>
| |
| - | </tr>
| |
| - | </table>
| |
| - | <table border=1>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">a</td><td width = "255px"><font color=red>HA2+3'UTR</font></td><td width = "200px">58*C</td><td width = "223px">100%</td><td width = "70px">531bp</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">b</td><td width = "255px"><font color=red>F_Fusion antibody receptor</font></td><td width = "200px">58*C</td><td width = "223px">100%</td><td width = "70px">1107bp</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">c</td><td width = "255px"><font color=red>F_Fusion antibody receptor</font></td><td width = "200px">58*C</td><td width = "223px">100%</td><td width = "70px">1107bp</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">d</td><td width = "255px"><font color=red>B_Fusion antibody receptor</font></td><td width = "200px">58*C</td><td width = "223px">100%</td><td width = "70px">1398bp</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">e</td><td width = "255px"><font color=red>B_Fusion antibody receptor</font></td><td width = "200px">58*C</td><td width = "223px">100%</td><td width = "70px">1398bp</td>
| |
| - | </tr>
| |
| - | </table>
| |
| - | <br>
| |
| - | [[Image:KAIST-exp27.png|360px]]
| |
| - | </center>
| |
| - | <br>
| |
| - | <br>
| |
| - |
| |
| - | == 9/19 PCR result ==
| |
| - |
| |
| - | <span style=font-size:12px><b>PCR composition </b></span>
| |
| - | <table border=1>
| |
| - | <tr align=center>
| |
| - | <td><b>Materials</b></td><td><b>Amount</b></td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td>Template</td><td>100pmole</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td>Primer A</td><td>50pmole</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td>Primer B</td><td>50pmole</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td>Bioneer Accupower HF PCR premix </td><td> < 1ul</td>
| |
| - | </tr>
| |
| - | <tr align=center><td>DW (3rd sterile water)</td><td>rest</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td>Total</td><td>50ul</td>
| |
| - | </tr>
| |
| - | </table>
| |
| - | <br>
| |
| - | <br>
| |
| - | <b>Exp 1</b>
| |
| - | <center>
| |
| - | <table border=1>
| |
| - | <tr align=center>
| |
| - | <td width = "300px"><b>Segment</b></td><td width = "200px"><b>Annealing Temperature</b></td><td width = "300px"><b>Result</b></td>
| |
| - | </tr>
| |
| - | </table>
| |
| - | <table border=1>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">a</td><td width = "255px">Fusion antibody receptor</td><td width = "200px">58*C</td><td width = "223px">50%</td><td width = "70px">2493bp</td>
| |
| - | </tr>
| |
| - | </table>
| |
| - | <br>
| |
| - | [[Image:KAIST-exp28.png|360px]]
| |
| - | </center>
| |
| - | <br>
| |
| - | <br>
| |
| - |
| |
| - | == 9/21 PCR result ==
| |
| - |
| |
| - | <span style=font-size:12px><b>PCR composition </b></span>
| |
| - | <table border=1>
| |
| - | <tr align=center>
| |
| - | <td><b>Materials</b></td><td><b>Amount</b></td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td>Template</td><td>100pmole</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td>Primer A</td><td>50pmole</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td>Primer B</td><td>50pmole</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td>Bioneer Accupower HF PCR premix </td><td> < 1ul</td>
| |
| - | </tr>
| |
| - | <tr align=center><td>DW (3rd sterile water)</td><td>rest</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td>Total</td><td>50ul</td>
| |
| - | </tr>
| |
| - | </table>
| |
| - | <br>
| |
| - | <br>
| |
| - | <b>Exp 1</b>
| |
| - | <center>
| |
| - | <table border=1>
| |
| - | <tr align=center>
| |
| - | <td width = "300px"><b>Segment</b></td><td width = "200px"><b>Annealing Temperature</b></td><td width = "300px"><b>Result</b></td>
| |
| - | </tr>
| |
| - | </table>
| |
| - | <table border=1>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">a</td><td width = "255px"><font color=red>F_Fusion antibody receptor</font></td><td width = "200px">58*C</td><td width = "223px">100%</td><td width = "70px">1107bp</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">b</td><td width = "255px"><font color=red>B_Fusion antibody receptor</font></td><td width = "200px">58*C</td><td width = "223px">100%</td><td width = "70px">1398bp</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">c</td><td width = "255px">Core promoter</td><td width = "200px">58*C</td><td width = "223px">0%</td><td width = "70px">--</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">d</td><td width = "255px">Selection marker(42)</td><td width = "200px">58*C</td><td width = "223px">0%</td><td width = "70px">--</td>
| |
| - | </tr>
| |
| - | </table>
| |
| - | <br>
| |
| - | [[Image:KAIST-exp29.png|360px]]
| |
| - | </center>
| |
| - | <br>
| |
| - | <br>
| |
| - | <b>Exp 2</b>
| |
| - | <center>
| |
| - | <table border=1>
| |
| - | <tr align=center>
| |
| - | <td width = "300px"><b>Segment</b></td><td width = "200px"><b>Annealing Temperature</b></td><td width = "300px"><b>Result</b></td>
| |
| - | </tr>
| |
| - | </table>
| |
| - | <table border=1>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">a</td><td width = "255px">Selection marker(41)</td><td width = "200px">58*C</td><td width = "223px">0%</td><td width = "70px">--</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td width = "40px">b</td><td width = "255px">Selection marker(42)</td><td width = "200px">58*C</td><td width = "223px">0%</td><td width = "70px">--</td>
| |
| - | </tr>
| |
| - | </table>
| |
| - | <br>
| |
| - | [[Image:KAIST-exp30.png|360px]]
| |
| - | </center>
| |
| - | <br>
| |
| - | <br>
| |
| - |
| |
| - | == 10/5 PCR result ==
| |
| - |
| |
| - | <span style=font-size:12px><b>PCR composition </b></span>
| |
| - | <table border=1>
| |
| - | <tr align=center>
| |
| - | <td><b>Materials</b></td><td><b>Amount</b></td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td>Template</td><td>100pmole</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td>Primer A</td><td>50pmole</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td>Primer B</td><td>50pmole</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td>Bioneer Accupower HF PCR premix </td><td> < 1ul</td>
| |
| - | </tr>
| |
| - | <tr align=center><td>DW (3rd sterile water)</td><td>rest</td>
| |
| - | </tr>
| |
| - | <tr align=center>
| |
| - | <td>Total</td><td>50ul</td>
| |
| - | </tr>
| |
| - | </table>
| |
| - | <br>
| |
| - | <br>
| |
| - | <b>Exp 1</b>
| |
| | <center> | | <center> |
| | <table border=1> | | <table border=1> |
"
