Team:KAIST-Korea/Project/Methods/PCR Result

From 2010.igem.org

(Difference between revisions)
(9/17 PCR result)
Line 1,779: Line 1,779:
== 10/5 PCR result ==
== 10/5 PCR result ==
-
 
-
<span style=font-size:12px><b>PCR composition </b></span>
 
-
<table border=1>
 
-
<tr align=center>
 
-
<td><b>Materials</b></td><td><b>Amount</b></td>
 
-
</tr>
 
-
<tr align=center>
 
-
<td>Template</td><td>100pmole</td>
 
-
</tr>
 
-
<tr align=center>
 
-
<td>Primer A</td><td>50pmole</td>
 
-
</tr>
 
-
<tr align=center>
 
-
<td>Primer B</td><td>50pmole</td>
 
-
</tr>
 
-
<tr align=center>
 
-
<td>Bioneer Accupower HF PCR premix </td><td> < 1ul</td>
 
-
</tr>
 
-
<tr align=center><td>DW (3rd sterile water)</td><td>rest</td>
 
-
</tr>
 
-
<tr align=center>
 
-
<td>Total</td><td>50ul</td>
 
-
</tr>
 
-
</table>
 
-
<br>
 
-
<br>
 
-
<b>Exp 1</b>
 
-
<table border=1>
 
-
<tr align=center>
 
-
<td width = "300px"><b>Segment</b></td><td width = "200px"><b>Annealing Temperature</b></td><td width = "300px"><b>Result</b></td>
 
-
</tr>
 
-
</table>
 
-
 
-
<table border=1>
 
-
<tr align=center>
 
-
<td width = "40px">a</td><td width = "255px">Selection marker(41)</td><td width = "200px">50*C</td><td width = "223px">0%</td><td width = "70px">--</td>
 
-
</tr>
 
-
<tr align=center>
 
-
<td width = "40px">b</td><td width = "255px">Selection marker(41)</td><td width = "200px">52*C</td><td width = "223px">0%</td><td width = "70px">--</td>
 
-
</tr>
 
-
<tr align=center>
 
-
<td width = "40px">c</td><td width = "255px">Selection marker(41)</td><td width = "200px">54*C</td><td width = "223px">0%</td><td width = "70px">--</td>
 
-
</tr>
 
-
<tr align=center>
 
-
<td width = "40px">d</td><td width = "255px">Selection marker(41)</td><td width = "200px">56*C</td><td width = "223px">0%</td><td width = "70px">--</td>
 
-
</tr>
 
-
<tr align=center>
 
-
<td width = "40px">e</td><td width = "255px">Selection marker(42)</td><td width = "200px">50*C</td><td width = "223px">0%</td><td width = "70px">--</td>
 
-
</tr>
 
-
<tr align=center>
 
-
<td width = "40px">f</td><td width = "255px">Selection marker(42)</td><td width = "200px">52*C</td><td width = "223px">0%</td><td width = "70px">--</td>
 
-
</tr>
 
-
<tr align=center>
 
-
<td width = "40px">g</td><td width = "255px">Selection marker(42)</td><td width = "200px">54*C</td><td width = "223px">0%</td><td width = "70px">--</td>
 
-
</tr>
 
-
<tr align=center>
 
-
<td width = "40px">h</td><td width = "255px">Selection marker(42)</td><td width = "200px">56*C</td><td width = "223px">0%</td><td width = "70px">--</td>
 
-
</tr>
 
-
<tr align=center>
 
-
<td width = "40px">i</td><td width = "255px">F_Fusion antibody receptor</td><td width = "200px">54*C</td><td width = "223px">30%</td><td width = "70px">1107bp</td>
 
-
</tr>
 
-
<tr align=center>
 
-
<td width = "40px">j</td><td width = "255px">F_Fusion antibody receptor</td><td width = "200px">54*C</td><td width = "223px">50%</td><td width = "70px">1107bp</td>
 
-
</tr>
 
-
<tr align=center>
 
-
<td width = "40px">k</td><td width = "255px">B_Fusion antibody receptor</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">1398bp</td>4
 
-
</tr>
 
-
<tr align=center>
 
-
<td width = "40px">l</td><td width = "255px">B_Fusion antibody receptor</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">1398bp</td>
 
-
</tr>
 
-
</table>
 
-
<br>
 
-
<br>
 
-
[[Image:KAIST-exp25.png|360px]]
 
-
<br>
 
-
<br>
 
-
<b>Exp 2</b>
 
-
<table border=1>
 
-
<tr align=center>
 
-
<td width = "300px"><b>Segment</b></td><td width = "200px"><b>Annealing Temperature</b></td><td width = "300px"><b>Result</b></td>
 
-
</tr>
 
-
</table>
 
-
<table border=1>
 
-
<tr align=center>
 
-
<td width = "40px">a</td><td width = "255px">HA1</td><td width = "200px">50*C</td><td width = "223px">100%</td><td width = "70px">87bp</td>
 
-
</tr>
 
-
<tr align=center>
 
-
<td width = "40px">b</td><td width = "255px">HA1</td><td width = "200px">52*C</td><td width = "223px">100%</td><td width = "70px">87bp</td>
 
-
</tr>
 
-
<tr align=center>
 
-
<td width = "40px">c</td><td width = "255px">HA1</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">87bp</td>
 
-
</tr>
 
-
<tr align=center>
 
-
<td width = "40px">d</td><td width = "255px">HA1</td><td width = "200px">56*C</td><td width = "223px">100%</td><td width = "70px">87bp</td>
 
-
</tr>
 
-
<tr align=center>
 
-
<td width = "40px">e</td><td width = "255px">HA2</td><td width = "200px">50*C</td><td width = "223px">80%</td><td width = "70px">87bp</td>
 
-
</tr>
 
-
<tr align=center>
 
-
<td width = "40px">f</td><td width = "255px">HA2</td><td width = "200px">52*C</td><td width = "223px">80%</td><td width = "70px">87bp</td>
 
-
</tr>
 
-
<tr align=center>
 
-
<td width = "40px">g</td><td width = "255px">HA2</td><td width = "200px">54*C</td><td width = "223px">80%</td><td width = "70px">87bp</td>
 
-
</tr>
 
-
<tr align=center>
 
-
<td width = "40px">h</td><td width = "255px">HA2</td><td width = "200px">56*C</td><td width = "223px">80%</td><td width = "70px">87bp</td>
 
-
</tr>
 
-
<tr align=center>
 
-
<td width = "40px">i</td><td width = "255px">Proximal promoter</td><td width = "200px">50*C</td><td width = "223px">100%</td><td width = "70px">120bp</td>
 
-
</tr>
 
-
<tr align=center>
 
-
<td width = "40px">j</td><td width = "255px">Proximal promoter</td><td width = "200px">52*C</td><td width = "223px">100%</td><td width = "70px">120bp</td>
 
-
</tr>
 
-
<tr align=center>
 
-
<td width = "40px">k</td><td width = "255px">Proximal promoter</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">120bp</td>
 
-
</tr>
 
-
<tr align=center>
 
-
<td width = "40px">l</td><td width = "255px">Proximal promoter</td><td width = "200px">56*C</td><td width = "223px">100%</td><td width = "70px">120bp</td>
 
-
</tr>
 
-
<tr align=center>
 
-
<td width = "40px">m</td><td width = "255px">5' UTR</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">500bp</td>
 
-
</tr>
 
-
<tr align=center>
 
-
<td width = "40px">n</td><td width = "255px">5' UTR</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">500bp</td>
 
-
</tr>
 
-
<tr align=center>
 
-
<td width = "40px">o</td><td width = "255px">3' UTR</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">444bp</td>
 
-
</tr>
 
-
<tr align=center>
 
-
<td width = "40px">p</td><td width = "255px">3' UTR</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">444bp</td>
 
-
</tr>
 
-
</table>
 
-
<br>
 
-
<br>
 
-
[[Image:KAIST-exp26.png|360px]]
 
-
<br>
 
-
<br>
 
-
 
-
== 9/17 PCR result ==
 
-
 
-
<span style=font-size:12px><b>PCR composition </b></span>
 
-
<table border=1>
 
-
<tr align=center>
 
-
<td><b>Materials</b></td><td><b>Amount</b></td>
 
-
</tr>
 
-
<tr align=center>
 
-
<td>Template</td><td>100pmole</td>
 
-
</tr>
 
-
<tr align=center>
 
-
<td>Primer A</td><td>50pmole</td>
 
-
</tr>
 
-
<tr align=center>
 
-
<td>Primer B</td><td>50pmole</td>
 
-
</tr>
 
-
<tr align=center>
 
-
<td>Bioneer Accupower HF PCR premix </td><td> < 1ul</td>
 
-
</tr>
 
-
<tr align=center><td>DW (3rd sterile water)</td><td>rest</td>
 
-
</tr>
 
-
<tr align=center>
 
-
<td>Total</td><td>50ul</td>
 
-
</tr>
 
-
</table>
 
-
<br>
 
-
<br>
 
-
<b>Exp 1</b>
 
-
<table border=1>
 
-
<tr align=center>
 
-
<td width = "300px"><b>Segment</b></td><td width = "200px"><b>Annealing Temperature</b></td><td width = "300px"><b>Result</b></td>
 
-
</tr>
 
-
</table>
 
-
 
-
<table border=1>
 
-
<tr align=center>
 
-
<td width = "40px">a</td><td width = "255px">Selection marker(41)</td><td width = "200px">50*C</td><td width = "223px">0%</td><td width = "70px">--</td>
 
-
</tr>
 
-
<tr align=center>
 
-
<td width = "40px">b</td><td width = "255px">Selection marker(41)</td><td width = "200px">52*C</td><td width = "223px">0%</td><td width = "70px">--</td>
 
-
</tr>
 
-
<tr align=center>
 
-
<td width = "40px">c</td><td width = "255px">Selection marker(41)</td><td width = "200px">54*C</td><td width = "223px">0%</td><td width = "70px">--</td>
 
-
</tr>
 
-
<tr align=center>
 
-
<td width = "40px">d</td><td width = "255px">Selection marker(41)</td><td width = "200px">56*C</td><td width = "223px">0%</td><td width = "70px">--</td>
 
-
</tr>
 
-
<tr align=center>
 
-
<td width = "40px">e</td><td width = "255px">Selection marker(42)</td><td width = "200px">50*C</td><td width = "223px">0%</td><td width = "70px">--</td>
 
-
</tr>
 
-
<tr align=center>
 
-
<td width = "40px">f</td><td width = "255px">Selection marker(42)</td><td width = "200px">52*C</td><td width = "223px">0%</td><td width = "70px">--</td>
 
-
</tr>
 
-
<tr align=center>
 
-
<td width = "40px">g</td><td width = "255px">Selection marker(42)</td><td width = "200px">54*C</td><td width = "223px">0%</td><td width = "70px">--</td>
 
-
</tr>
 
-
<tr align=center>
 
-
<td width = "40px">h</td><td width = "255px">Selection marker(42)</td><td width = "200px">56*C</td><td width = "223px">0%</td><td width = "70px">--</td>
 
-
</tr>
 
-
<tr align=center>
 
-
<td width = "40px">i</td><td width = "255px">F_Fusion antibody receptor</td><td width = "200px">54*C</td><td width = "223px">30%</td><td width = "70px">1107bp</td>
 
-
</tr>
 
-
<tr align=center>
 
-
<td width = "40px">j</td><td width = "255px">F_Fusion antibody receptor</td><td width = "200px">54*C</td><td width = "223px">50%</td><td width = "70px">1107bp</td>
 
-
</tr>
 
-
<tr align=center>
 
-
<td width = "40px">k</td><td width = "255px">B_Fusion antibody receptor</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">1398bp</td>4
 
-
</tr>
 
-
<tr align=center>
 
-
<td width = "40px">l</td><td width = "255px">B_Fusion antibody receptor</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">1398bp</td>
 
-
</tr>
 
-
</table>
 
-
<br>
 
-
<br>
 
-
[[Image:KAIST-exp25.png|360px]]
 
-
<br>
 
-
<br>
 
-
<b>Exp 2</b>
 
-
<table border=1>
 
-
<tr align=center>
 
-
<td width = "300px"><b>Segment</b></td><td width = "200px"><b>Annealing Temperature</b></td><td width = "300px"><b>Result</b></td>
 
-
</tr>
 
-
</table>
 
-
<table border=1>
 
-
<tr align=center>
 
-
<td width = "40px">a</td><td width = "255px">HA1</td><td width = "200px">50*C</td><td width = "223px">100%</td><td width = "70px">87bp</td>
 
-
</tr>
 
-
<tr align=center>
 
-
<td width = "40px">b</td><td width = "255px">HA1</td><td width = "200px">52*C</td><td width = "223px">100%</td><td width = "70px">87bp</td>
 
-
</tr>
 
-
<tr align=center>
 
-
<td width = "40px">c</td><td width = "255px">HA1</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">87bp</td>
 
-
</tr>
 
-
<tr align=center>
 
-
<td width = "40px">d</td><td width = "255px">HA1</td><td width = "200px">56*C</td><td width = "223px">100%</td><td width = "70px">87bp</td>
 
-
</tr>
 
-
<tr align=center>
 
-
<td width = "40px">e</td><td width = "255px">HA2</td><td width = "200px">50*C</td><td width = "223px">80%</td><td width = "70px">87bp</td>
 
-
</tr>
 
-
<tr align=center>
 
-
<td width = "40px">f</td><td width = "255px">HA2</td><td width = "200px">52*C</td><td width = "223px">80%</td><td width = "70px">87bp</td>
 
-
</tr>
 
-
<tr align=center>
 
-
<td width = "40px">g</td><td width = "255px">HA2</td><td width = "200px">54*C</td><td width = "223px">80%</td><td width = "70px">87bp</td>
 
-
</tr>
 
-
<tr align=center>
 
-
<td width = "40px">h</td><td width = "255px">HA2</td><td width = "200px">56*C</td><td width = "223px">80%</td><td width = "70px">87bp</td>
 
-
</tr>
 
-
<tr align=center>
 
-
<td width = "40px">i</td><td width = "255px">Proximal promoter</td><td width = "200px">50*C</td><td width = "223px">100%</td><td width = "70px">120bp</td>
 
-
</tr>
 
-
<tr align=center>
 
-
<td width = "40px">j</td><td width = "255px">Proximal promoter</td><td width = "200px">52*C</td><td width = "223px">100%</td><td width = "70px">120bp</td>
 
-
</tr>
 
-
<tr align=center>
 
-
<td width = "40px">k</td><td width = "255px">Proximal promoter</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">120bp</td>
 
-
</tr>
 
-
<tr align=center>
 
-
<td width = "40px">l</td><td width = "255px">Proximal promoter</td><td width = "200px">56*C</td><td width = "223px">100%</td><td width = "70px">120bp</td>
 
-
</tr>
 
-
<tr align=center>
 
-
<td width = "40px">m</td><td width = "255px">5' UTR</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">500bp</td>
 
-
</tr>
 
-
<tr align=center>
 
-
<td width = "40px">n</td><td width = "255px">5' UTR</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">500bp</td>
 
-
</tr>
 
-
<tr align=center>
 
-
<td width = "40px">o</td><td width = "255px">3' UTR</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">444bp</td>
 
-
</tr>
 
-
<tr align=center>
 
-
<td width = "40px">p</td><td width = "255px">3' UTR</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">444bp</td>
 
-
</tr>
 
-
</table>
 
-
<br>
 
-
<br>
 
-
[[Image:KAIST-exp26.png|360px]]
 
-
<br>
 
-
<br>
 
-
 
-
 
-
== 9/17 PCR result ==
 
-
 
-
<span style=font-size:12px><b>PCR composition </b></span>
 
-
<table border=1>
 
-
<tr align=center>
 
-
<td><b>Materials</b></td><td><b>Amount</b></td>
 
-
</tr>
 
-
<tr align=center>
 
-
<td>Template</td><td>100pmole</td>
 
-
</tr>
 
-
<tr align=center>
 
-
<td>Primer A</td><td>50pmole</td>
 
-
</tr>
 
-
<tr align=center>
 
-
<td>Primer B</td><td>50pmole</td>
 
-
</tr>
 
-
<tr align=center>
 
-
<td>Bioneer Accupower HF PCR premix </td><td> < 1ul</td>
 
-
</tr>
 
-
<tr align=center><td>DW (3rd sterile water)</td><td>rest</td>
 
-
</tr>
 
-
<tr align=center>
 
-
<td>Total</td><td>50ul</td>
 
-
</tr>
 
-
</table>
 
-
<br>
 
-
<br>
 
-
<b>Exp 1</b>
 
-
<table border=1>
 
-
<tr align=center>
 
-
<td width = "300px"><b>Segment</b></td><td width = "200px"><b>Annealing Temperature</b></td><td width = "300px"><b>Result</b></td>
 
-
</tr>
 
-
</table>
 
-
 
-
<table border=1>
 
-
<tr align=center>
 
-
<td width = "40px">a</td><td width = "255px">Selection marker(41)</td><td width = "200px">50*C</td><td width = "223px">0%</td><td width = "70px">--</td>
 
-
</tr>
 
-
<tr align=center>
 
-
<td width = "40px">b</td><td width = "255px">Selection marker(41)</td><td width = "200px">52*C</td><td width = "223px">0%</td><td width = "70px">--</td>
 
-
</tr>
 
-
<tr align=center>
 
-
<td width = "40px">c</td><td width = "255px">Selection marker(41)</td><td width = "200px">54*C</td><td width = "223px">0%</td><td width = "70px">--</td>
 
-
</tr>
 
-
<tr align=center>
 
-
<td width = "40px">d</td><td width = "255px">Selection marker(41)</td><td width = "200px">56*C</td><td width = "223px">0%</td><td width = "70px">--</td>
 
-
</tr>
 
-
<tr align=center>
 
-
<td width = "40px">e</td><td width = "255px">Selection marker(42)</td><td width = "200px">50*C</td><td width = "223px">0%</td><td width = "70px">--</td>
 
-
</tr>
 
-
<tr align=center>
 
-
<td width = "40px">f</td><td width = "255px">Selection marker(42)</td><td width = "200px">52*C</td><td width = "223px">0%</td><td width = "70px">--</td>
 
-
</tr>
 
-
<tr align=center>
 
-
<td width = "40px">g</td><td width = "255px">Selection marker(42)</td><td width = "200px">54*C</td><td width = "223px">0%</td><td width = "70px">--</td>
 
-
</tr>
 
-
<tr align=center>
 
-
<td width = "40px">h</td><td width = "255px">Selection marker(42)</td><td width = "200px">56*C</td><td width = "223px">0%</td><td width = "70px">--</td>
 
-
</tr>
 
-
<tr align=center>
 
-
<td width = "40px">i</td><td width = "255px">F_Fusion antibody receptor</td><td width = "200px">54*C</td><td width = "223px">30%</td><td width = "70px">1107bp</td>
 
-
</tr>
 
-
<tr align=center>
 
-
<td width = "40px">j</td><td width = "255px">F_Fusion antibody receptor</td><td width = "200px">54*C</td><td width = "223px">50%</td><td width = "70px">1107bp</td>
 
-
</tr>
 
-
<tr align=center>
 
-
<td width = "40px">k</td><td width = "255px">B_Fusion antibody receptor</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">1398bp</td>4
 
-
</tr>
 
-
<tr align=center>
 
-
<td width = "40px">l</td><td width = "255px">B_Fusion antibody receptor</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">1398bp</td>
 
-
</tr>
 
-
</table>
 
-
<br>
 
-
<br>
 
-
[[Image:KAIST-exp25.png|360px]]
 
-
<br>
 
-
<br>
 
-
<b>Exp 2</b>
 
-
<table border=1>
 
-
<tr align=center>
 
-
<td width = "300px"><b>Segment</b></td><td width = "200px"><b>Annealing Temperature</b></td><td width = "300px"><b>Result</b></td>
 
-
</tr>
 
-
</table>
 
-
<table border=1>
 
-
<tr align=center>
 
-
<td width = "40px">a</td><td width = "255px">HA1</td><td width = "200px">50*C</td><td width = "223px">100%</td><td width = "70px">87bp</td>
 
-
</tr>
 
-
<tr align=center>
 
-
<td width = "40px">b</td><td width = "255px">HA1</td><td width = "200px">52*C</td><td width = "223px">100%</td><td width = "70px">87bp</td>
 
-
</tr>
 
-
<tr align=center>
 
-
<td width = "40px">c</td><td width = "255px">HA1</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">87bp</td>
 
-
</tr>
 
-
<tr align=center>
 
-
<td width = "40px">d</td><td width = "255px">HA1</td><td width = "200px">56*C</td><td width = "223px">100%</td><td width = "70px">87bp</td>
 
-
</tr>
 
-
<tr align=center>
 
-
<td width = "40px">e</td><td width = "255px">HA2</td><td width = "200px">50*C</td><td width = "223px">80%</td><td width = "70px">87bp</td>
 
-
</tr>
 
-
<tr align=center>
 
-
<td width = "40px">f</td><td width = "255px">HA2</td><td width = "200px">52*C</td><td width = "223px">80%</td><td width = "70px">87bp</td>
 
-
</tr>
 
-
<tr align=center>
 
-
<td width = "40px">g</td><td width = "255px">HA2</td><td width = "200px">54*C</td><td width = "223px">80%</td><td width = "70px">87bp</td>
 
-
</tr>
 
-
<tr align=center>
 
-
<td width = "40px">h</td><td width = "255px">HA2</td><td width = "200px">56*C</td><td width = "223px">80%</td><td width = "70px">87bp</td>
 
-
</tr>
 
-
<tr align=center>
 
-
<td width = "40px">i</td><td width = "255px">Proximal promoter</td><td width = "200px">50*C</td><td width = "223px">100%</td><td width = "70px">120bp</td>
 
-
</tr>
 
-
<tr align=center>
 
-
<td width = "40px">j</td><td width = "255px">Proximal promoter</td><td width = "200px">52*C</td><td width = "223px">100%</td><td width = "70px">120bp</td>
 
-
</tr>
 
-
<tr align=center>
 
-
<td width = "40px">k</td><td width = "255px">Proximal promoter</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">120bp</td>
 
-
</tr>
 
-
<tr align=center>
 
-
<td width = "40px">l</td><td width = "255px">Proximal promoter</td><td width = "200px">56*C</td><td width = "223px">100%</td><td width = "70px">120bp</td>
 
-
</tr>
 
-
<tr align=center>
 
-
<td width = "40px">m</td><td width = "255px">5' UTR</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">500bp</td>
 
-
</tr>
 
-
<tr align=center>
 
-
<td width = "40px">n</td><td width = "255px">5' UTR</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">500bp</td>
 
-
</tr>
 
-
<tr align=center>
 
-
<td width = "40px">o</td><td width = "255px">3' UTR</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">444bp</td>
 
-
</tr>
 
-
<tr align=center>
 
-
<td width = "40px">p</td><td width = "255px">3' UTR</td><td width = "200px">54*C</td><td width = "223px">100%</td><td width = "70px">444bp</td>
 
-
</tr>
 
-
</table>
 
-
<br>
 
-
<br>
 
-
[[Image:KAIST-exp26.png|360px]]
 
-
<br>
 
-
<br>
 
-
 
-
 
-
== 9/17 PCR result ==
 
<span style=font-size:12px><b>PCR composition </b></span>
<span style=font-size:12px><b>PCR composition </b></span>

Revision as of 03:29, 17 October 2010

 



PCR Experiment

9/6 ~ 9/9 PCR Result

PCR composition

MaterialsAmount
Template100pmole
Primer A50pmole
Primer B50pmole
dNTP (dNTP mixture 2.5mM TaKaRa)2ul
Taq (Pfu Turbo® DNA Polymerase 2.5U/ μl STRATAGENE)0.25ul
10x buffer (10x cloned Pfu Reaction buffer)5ul
DW (3rd sterile water)rest
Total50ul



Exp 1

SegmentAnnealing TemperatureResult
aSTAT153*C--
bV_STAT153*C--
cFusion Antibody receptor53*C--
dSignal peptide56*C--
eIg-like56*C--
fSignal Peptide + Ig-like56*C--
gAntibody56*C--
hV_Fusion Antibody Receptor53*C--
iB_FGFR156*C--
jVB_FGFR156*C--



KAIST-exp1.png




Exp 2

SegmentAnnealing TemperatureResult
aSTAT148*C60%2139bp
bV_STAT148*C--



KAIST-exp2.png




Exp 3

SegmentAnnealing TemperatureResult
aSTAT150*C100%2139bp
bV_STAT150*C--



KAIST-exp3.png




Exp 4

SegmentAnnealing TemperatureResult
aFusion Antibody receptor50*C--
bSignal peptide50*C--
cIg-like50*C60%339bp
dSignal Peptide + Ig-like50*C--
eAntibody50*C--
fV_Fusion Antibody Receptor50*C--



KAIST-exp4.png






9/10 ~ 9/12 PCR Result

PCR composition

MaterialsAmount
Template150pmole
Primer A50pmole
Primer B50pmole
dNTP (dNTP mixture 2.5mM TaKaRa)5ul
Taq (Pfu Turbo® DNA Polymerase 2.5U/ μl STRATAGENE)0.25ul
10x buffer (10x cloned Pfu Reaction buffer)5ul
DW (3rd sterile water)rest
Total50ul



Exp 1

SegmentAnnealing TemperatureResult
aSTAT160*C--
bV_STAT160*C--
cFusion Antibody receptor60*C70%1095bp
dSignal peptide60*C--
eIg-like60*C60%339bp
fSignal Peptide + Ig-like60*C--
gAntibody60*C100%669bp
hV_Fusion Antibody Receptor60*C--
iB_FGFR160*C--
jVB_FGFR160*C--
x1negative 160*C--
x2negative 260*C--



KAIST-exp5.png





Exp 2

SegmentAnnealing TemperatureResult
aSTAT158*C60%2139bp
bV_STAT158*C--
cFusion Antibody receptor58*C80%1095bp
dSignal peptide58*C80%87bp
eIg-like58*C100%339bp
fSignal Peptide + Ig-like58*C80%426bp
gAntibody58*C100%669bp
hV_Fusion Antibody Receptor58*C--
iB_FGFR158*C--
jVB_FGFR158*C--
xnegative58*C--



KAIST-exp6.png




Exp 3

SegmentAnnealing TemperatureResult
aSTAT162*C--
bV_STAT162*C--
cFusion Antibody receptor62*C10%1095bp
dSignal peptide62*C--
eIg-like62*C--
fSignal Peptide + Ig-like62*C--
gAntibody62*C--
hV_Fusion Antibody Receptor62*C--
iB_FGFR162*C--
jVB_FGFR162*C--
xnegative62*C--



KAIST-exp7.png




Exp 4

SegmentAnnealing TemperatureResult
aSTAT156*C--
bV_STAT156*C--
cFusion Antibody receptor56*C5%1095bp
dSignal peptide56*C--
eIg-like56*C80%339bp
fSignal Peptide + Ig-like56*C--
gAntibody56*C80%669bp
hV_Fusion Antibody Receptor56*C--
iB_FGFR156*C--
jVB_FGFR156*C--
xnegative56*C--



KAIST-exp8.png




Exp 5

SegmentAnnealing TemperatureResult
aV_STAT164*C--
bB_FGFR164*C5%1398bp
cVB_FGFR164*C--



KAIST-exp9.png





Exp 6

SegmentAnnealing TemperatureResult
aV_STAT164*C--
bB_FGFR164*C3%1398bp
cVB_FGFR164*C--



KAIST-exp10.png





Exp 7

SegmentAnnealing TemperatureResult
a3'UTR62*C--
b5'UTR62*C--









Exp 8

SegmentAnnealing TemperatureResult
aV_STAT168*C--
bB_FGFR168*C10%1398bp
cVB_FGFR168*C--



KAIST-exp12.png





Exp 9

SegmentAnnealing TemperatureResult
aV_STAT170*C--
bB_FGFR170*C3%1398bp
cVB_FGFR170*C--
c5'UTR70*C--
c3'UTR70*C--



KAIST-exp13.png





Exp 10

SegmentAnnealing TemperatureResult
aV_STAT167*C--
bB_FGFR167*C6%1398bp
cVB_FGFR167*C--



KAIST-exp14.png





9/13 ~ 9/16 PCR Result

PCR composition

MaterialsAmount
Template100pmole
Primer A50pmole
Primer B50pmole
Bioneer Accupower HF PCR premix < 1ul
DW (3rd sterile water)rest
Total50ul



Exp 1

SegmentAnnealing TemperatureResult
aSTAT150*C100%2139bp
bSTAT152*C100%2139bp
cSTAT154*C100%2139bp
dSTAT156*C100%2139bp
eSTAT158*C100%2139bp
fSTAT160*C100%2139bp
gV_STAT150*C100%2151bp
hV_STAT152*C100%2151bp
iV_STAT154*C100%2151bp
jV_STAT156*C100%2151bp
kV_STAT158*C100%2151bp
lV_STAT160*C100%2151bp



KAIST-exp21.png




Exp 2

SegmentAnnealing TemperatureResult
aB_FGFR50*C100%1398bp
bB_FGFR52*C100%1398bp
cB_FGFR54*C100%1398bp
dB_FGFR56*C100%1398bp
eB_FGFR58*C100%1398bp
fB_FGFR60*C100%1398bp
gVB_FGFR50*C100%1420bp
hVB_FGFR52*C100%1420bp
iVB_FGFR54*C100%1420bp
jVB_FGFR56*C100%1420bp
kVB_FGFR58*C100%1420bp
lVB_FGFR60*C100%1420bp



KAIST-exp22.png




Exp 3

SegmentAnnealing TemperatureResult
aSignal peptide61*C30%87bp
bIg-like61*C100%339bp
cSignal peptide + Ig-like61*C100%426bp
dSignal peptide59*C30%87bp
eIg-like59*C100%339bp
fSignal peptide + Ig-like59*C100%426bp
g3'UTR52*C50%444bp
h3'UTR54*C50%444bp
i3'UTR56*C100%444bp
j3'UTR58*C100%444bp
k3'UTR60*C100%444bp
l5'UTR52*C100%500bp
m5'UTR54*C100%500bp
n5'UTR56*C100%500bp
o5'UTR58*C100%500bp
p5'UTR69*C70%500bp



KAIST-exp23.png




Exp 4

SegmentAnnealing TemperatureResult
aF_Fusion antibody receptor60*C100%1095bp
bF_Fusion antibody receptor58*C100%1095bp
cAntibody60*C100%669bp
dAntibody58*C100%669bp
eVF_Fusion antibody receptor60*C100%1107bp
fVF_Fusion antibody receptor58*C100%1107bp



KAIST-exp24.png




9/17 PCR result

PCR composition

MaterialsAmount
Template100pmole
Primer A50pmole
Primer B50pmole
Bioneer Accupower HF PCR premix < 1ul
DW (3rd sterile water)rest
Total50ul



Exp 1

SegmentAnnealing TemperatureResult
4
aSelection marker(41)50*C0%--
bSelection marker(41)52*C0%--
cSelection marker(41)54*C0%--
dSelection marker(41)56*C0%--
eSelection marker(42)50*C0%--
fSelection marker(42)52*C0%--
gSelection marker(42)54*C0%--
hSelection marker(42)56*C0%--
iF_Fusion antibody receptor54*C30%1107bp
jF_Fusion antibody receptor54*C50%1107bp
kB_Fusion antibody receptor54*C100%1398bp
lB_Fusion antibody receptor54*C100%1398bp



KAIST-exp25.png

Exp 2

SegmentAnnealing TemperatureResult
aHA150*C100%87bp
bHA152*C100%87bp
cHA154*C100%87bp
dHA156*C100%87bp
eHA250*C80%87bp
fHA252*C80%87bp
gHA254*C80%87bp
hHA256*C80%87bp
iProximal promoter50*C100%120bp
jProximal promoter52*C100%120bp
kProximal promoter54*C100%120bp
lProximal promoter56*C100%120bp
m5' UTR54*C100%500bp
n5' UTR54*C100%500bp
o3' UTR54*C100%444bp
p3' UTR54*C100%444bp



KAIST-exp26.png

9/18 PCR result

PCR composition

MaterialsAmount
Template100pmole
Primer A50pmole
Primer B50pmole
Bioneer Accupower HF PCR premix < 1ul
DW (3rd sterile water)rest
Total50ul



Exp 1

SegmentAnnealing TemperatureResult
4
aSelection marker(41)50*C0%--
bSelection marker(41)52*C0%--
cSelection marker(41)54*C0%--
dSelection marker(41)56*C0%--
eSelection marker(42)50*C0%--
fSelection marker(42)52*C0%--
gSelection marker(42)54*C0%--
hSelection marker(42)56*C0%--
iF_Fusion antibody receptor54*C30%1107bp
jF_Fusion antibody receptor54*C50%1107bp
kB_Fusion antibody receptor54*C100%1398bp
lB_Fusion antibody receptor54*C100%1398bp



KAIST-exp25.png

Exp 2

SegmentAnnealing TemperatureResult
aHA150*C100%87bp
bHA152*C100%87bp
cHA154*C100%87bp
dHA156*C100%87bp
eHA250*C80%87bp
fHA252*C80%87bp
gHA254*C80%87bp
hHA256*C80%87bp
iProximal promoter50*C100%120bp
jProximal promoter52*C100%120bp
kProximal promoter54*C100%120bp
lProximal promoter56*C100%120bp
m5' UTR54*C100%500bp
n5' UTR54*C100%500bp
o3' UTR54*C100%444bp
p3' UTR54*C100%444bp



KAIST-exp26.png

9/19 PCR result

PCR composition

MaterialsAmount
Template100pmole
Primer A50pmole
Primer B50pmole
Bioneer Accupower HF PCR premix < 1ul
DW (3rd sterile water)rest
Total50ul



Exp 1

SegmentAnnealing TemperatureResult
4
aSelection marker(41)50*C0%--
bSelection marker(41)52*C0%--
cSelection marker(41)54*C0%--
dSelection marker(41)56*C0%--
eSelection marker(42)50*C0%--
fSelection marker(42)52*C0%--
gSelection marker(42)54*C0%--
hSelection marker(42)56*C0%--
iF_Fusion antibody receptor54*C30%1107bp
jF_Fusion antibody receptor54*C50%1107bp
kB_Fusion antibody receptor54*C100%1398bp
lB_Fusion antibody receptor54*C100%1398bp



KAIST-exp25.png

Exp 2

SegmentAnnealing TemperatureResult
aHA150*C100%87bp
bHA152*C100%87bp
cHA154*C100%87bp
dHA156*C100%87bp
eHA250*C80%87bp
fHA252*C80%87bp
gHA254*C80%87bp
hHA256*C80%87bp
iProximal promoter50*C100%120bp
jProximal promoter52*C100%120bp
kProximal promoter54*C100%120bp
lProximal promoter56*C100%120bp
m5' UTR54*C100%500bp
n5' UTR54*C100%500bp
o3' UTR54*C100%444bp
p3' UTR54*C100%444bp



KAIST-exp26.png

9/21 PCR result

PCR composition

MaterialsAmount
Template100pmole
Primer A50pmole
Primer B50pmole
Bioneer Accupower HF PCR premix < 1ul
DW (3rd sterile water)rest
Total50ul



Exp 1

SegmentAnnealing TemperatureResult
4
aSelection marker(41)50*C0%--
bSelection marker(41)52*C0%--
cSelection marker(41)54*C0%--
dSelection marker(41)56*C0%--
eSelection marker(42)50*C0%--
fSelection marker(42)52*C0%--
gSelection marker(42)54*C0%--
hSelection marker(42)56*C0%--
iF_Fusion antibody receptor54*C30%1107bp
jF_Fusion antibody receptor54*C50%1107bp
kB_Fusion antibody receptor54*C100%1398bp
lB_Fusion antibody receptor54*C100%1398bp



KAIST-exp25.png

Exp 2

SegmentAnnealing TemperatureResult
aHA150*C100%87bp
bHA152*C100%87bp
cHA154*C100%87bp
dHA156*C100%87bp
eHA250*C80%87bp
fHA252*C80%87bp
gHA254*C80%87bp
hHA256*C80%87bp
iProximal promoter50*C100%120bp
jProximal promoter52*C100%120bp
kProximal promoter54*C100%120bp
lProximal promoter56*C100%120bp
m5' UTR54*C100%500bp
n5' UTR54*C100%500bp
o3' UTR54*C100%444bp
p3' UTR54*C100%444bp



KAIST-exp26.png

9/27 PCR result

PCR composition

MaterialsAmount
Template100pmole
Primer A50pmole
Primer B50pmole
Bioneer Accupower HF PCR premix < 1ul
DW (3rd sterile water)rest
Total50ul



Exp 1

SegmentAnnealing TemperatureResult
4
aSelection marker(41)50*C0%--
bSelection marker(41)52*C0%--
cSelection marker(41)54*C0%--
dSelection marker(41)56*C0%--
eSelection marker(42)50*C0%--
fSelection marker(42)52*C0%--
gSelection marker(42)54*C0%--
hSelection marker(42)56*C0%--
iF_Fusion antibody receptor54*C30%1107bp
jF_Fusion antibody receptor54*C50%1107bp
kB_Fusion antibody receptor54*C100%1398bp
lB_Fusion antibody receptor54*C100%1398bp



KAIST-exp25.png

Exp 2

SegmentAnnealing TemperatureResult
aHA150*C100%87bp
bHA152*C100%87bp
cHA154*C100%87bp
dHA156*C100%87bp
eHA250*C80%87bp
fHA252*C80%87bp
gHA254*C80%87bp
hHA256*C80%87bp
iProximal promoter50*C100%120bp
jProximal promoter52*C100%120bp
kProximal promoter54*C100%120bp
lProximal promoter56*C100%120bp
m5' UTR54*C100%500bp
n5' UTR54*C100%500bp
o3' UTR54*C100%444bp
p3' UTR54*C100%444bp



KAIST-exp26.png

9/28 PCR result

PCR composition

MaterialsAmount
Template100pmole
Primer A50pmole
Primer B50pmole
Bioneer Accupower HF PCR premix < 1ul
DW (3rd sterile water)rest
Total50ul



Exp 1

SegmentAnnealing TemperatureResult
4
aSelection marker(41)50*C0%--
bSelection marker(41)52*C0%--
cSelection marker(41)54*C0%--
dSelection marker(41)56*C0%--
eSelection marker(42)50*C0%--
fSelection marker(42)52*C0%--
gSelection marker(42)54*C0%--
hSelection marker(42)56*C0%--
iF_Fusion antibody receptor54*C30%1107bp
jF_Fusion antibody receptor54*C50%1107bp
kB_Fusion antibody receptor54*C100%1398bp
lB_Fusion antibody receptor54*C100%1398bp



KAIST-exp25.png

Exp 2

SegmentAnnealing TemperatureResult
aHA150*C100%87bp
bHA152*C100%87bp
cHA154*C100%87bp
dHA156*C100%87bp
eHA250*C80%87bp
fHA252*C80%87bp
gHA254*C80%87bp
hHA256*C80%87bp
iProximal promoter50*C100%120bp
jProximal promoter52*C100%120bp
kProximal promoter54*C100%120bp
lProximal promoter56*C100%120bp
m5' UTR54*C100%500bp
n5' UTR54*C100%500bp
o3' UTR54*C100%444bp
p3' UTR54*C100%444bp



KAIST-exp26.png

10/1 PCR result

PCR composition

MaterialsAmount
Template100pmole
Primer A50pmole
Primer B50pmole
Bioneer Accupower HF PCR premix < 1ul
DW (3rd sterile water)rest
Total50ul



Exp 1

SegmentAnnealing TemperatureResult
4
aSelection marker(41)50*C0%--
bSelection marker(41)52*C0%--
cSelection marker(41)54*C0%--
dSelection marker(41)56*C0%--
eSelection marker(42)50*C0%--
fSelection marker(42)52*C0%--
gSelection marker(42)54*C0%--
hSelection marker(42)56*C0%--
iF_Fusion antibody receptor54*C30%1107bp
jF_Fusion antibody receptor54*C50%1107bp
kB_Fusion antibody receptor54*C100%1398bp
lB_Fusion antibody receptor54*C100%1398bp



KAIST-exp25.png

Exp 2

SegmentAnnealing TemperatureResult
aHA150*C100%87bp
bHA152*C100%87bp
cHA154*C100%87bp
dHA156*C100%87bp
eHA250*C80%87bp
fHA252*C80%87bp
gHA254*C80%87bp
hHA256*C80%87bp
iProximal promoter50*C100%120bp
jProximal promoter52*C100%120bp
kProximal promoter54*C100%120bp
lProximal promoter56*C100%120bp
m5' UTR54*C100%500bp
n5' UTR54*C100%500bp
o3' UTR54*C100%444bp
p3' UTR54*C100%444bp



KAIST-exp26.png

10/5 PCR result

PCR composition

MaterialsAmount
Template100pmole
Primer A50pmole
Primer B50pmole
Bioneer Accupower HF PCR premix < 1ul
DW (3rd sterile water)rest
Total50ul



Exp 1

SegmentAnnealing TemperatureResult
4
aSelection marker(41)50*C0%--
bSelection marker(41)52*C0%--
cSelection marker(41)54*C0%--
dSelection marker(41)56*C0%--
eSelection marker(42)50*C0%--
fSelection marker(42)52*C0%--
gSelection marker(42)54*C0%--
hSelection marker(42)56*C0%--
iF_Fusion antibody receptor54*C30%1107bp
jF_Fusion antibody receptor54*C50%1107bp
kB_Fusion antibody receptor54*C100%1398bp
lB_Fusion antibody receptor54*C100%1398bp



KAIST-exp25.png

Exp 2

SegmentAnnealing TemperatureResult
aHA150*C100%87bp
bHA152*C100%87bp
cHA154*C100%87bp
dHA156*C100%87bp
eHA250*C80%87bp
fHA252*C80%87bp
gHA254*C80%87bp
hHA256*C80%87bp
iProximal promoter50*C100%120bp
jProximal promoter52*C100%120bp
kProximal promoter54*C100%120bp
lProximal promoter56*C100%120bp
m5' UTR54*C100%500bp
n5' UTR54*C100%500bp
o3' UTR54*C100%444bp
p3' UTR54*C100%444bp



KAIST-exp26.png