Team:KAIST-Korea/Notebook/Memo/Data
From 2010.igem.org
(19 intermediate revisions not shown) | |||
Line 9: | Line 9: | ||
<table width="100%"> | <table width="100%"> | ||
<tr> | <tr> | ||
- | <td> | + | <td align=center> |
<html><a href="https://2010.igem.org/Team:KAIST-Korea/Notebook/Memo/Data"><img src="https://static.igem.org/mediawiki/2010/thumb/5/54/Data.jpg/230px-Data.jpg"></a></html> | <html><a href="https://2010.igem.org/Team:KAIST-Korea/Notebook/Memo/Data"><img src="https://static.igem.org/mediawiki/2010/thumb/5/54/Data.jpg/230px-Data.jpg"></a></html> | ||
<html><a href="https://2010.igem.org/Team:KAIST-Korea/Notebook/Memo/Info"><img src="https://static.igem.org/mediawiki/2010/thumb/e/e9/Info.jpg/230px-Info.jpg"></a></html> | <html><a href="https://2010.igem.org/Team:KAIST-Korea/Notebook/Memo/Info"><img src="https://static.igem.org/mediawiki/2010/thumb/e/e9/Info.jpg/230px-Info.jpg"></a></html> | ||
Line 17: | Line 17: | ||
</tr> | </tr> | ||
</table> | </table> | ||
+ | |||
+ | <table width="100%" border="0" cellpadding="20px"> | ||
+ | <tr> | ||
+ | <td> | ||
== <span style=font-size:20px> <b> Data </b> </span> == | == <span style=font-size:20px> <b> Data </b> </span> == | ||
<br> | <br> | ||
Line 27: | Line 31: | ||
<span style=font-size:15px> <b> Protein information for virus-inspired Drug delivery system. </b> </span> | <span style=font-size:15px> <b> Protein information for virus-inspired Drug delivery system. </b> </span> | ||
<br> | <br> | ||
- | |||
- | |||
HNMT | HNMT | ||
Entrez ID : 3176 | Entrez ID : 3176 | ||
- | + | :related mRNA or protein sequence | |
NM_001024074.2 → NP_001019245.1 | NM_001024074.2 → NP_001019245.1 | ||
- | + | :histamine N-methyltransferase isoform 2 | |
NM_001024075.1 → NP_001019246.1 | NM_001024075.1 → NP_001019246.1 | ||
- | + | :histamine N-methyltransferase isoform 3 | |
NM_006895.2 → NP_008826.1 | NM_006895.2 → NP_008826.1 | ||
- | + | :histamine N-methyltransferase isoform 1 | |
(3 kinds of isoform is obtained by alternative splicing) | (3 kinds of isoform is obtained by alternative splicing) | ||
Human Coronaviral Spike protein. | Human Coronaviral Spike protein. | ||
- | + | :NL63 strain | |
- | + | Nucleotide sequence is in | |
- | + | :http://www.ncbi.nlm.nih.gov/nuccore/49169782/?from=20472&to=24542&report=fasta (Gene ID : 2943499) | |
- | + | protein sequence is in | |
- | http://www.ncbi.nlm.nih.gov/protein/45655909?report=genpept | + | :http://www.ncbi.nlm.nih.gov/protein/45655909?report=genpept |
- | + | SARS starin | |
- | + | nucleotide sequence is in | |
- | http://www.ncbi.nlm.nih.gov/nuccore/30271926/?from=21492&to=25259&report=fasta (Gene ID : 1489668) | + | :http://www.ncbi.nlm.nih.gov/nuccore/30271926/?from=21492&to=25259&report=fasta (Gene ID : 1489668) |
- | + | protein sequence is in | |
- | http://www.ncbi.nlm.nih.gov/protein/29836496?report=genpept | + | :http://www.ncbi.nlm.nih.gov/protein/29836496?report=genpept |
<br><br> | <br><br> | ||
+ | <span style=font-size:15px> <b> SARS-CoV Spike Protein </b> </span><br> | ||
+ | It seems very hard to get SARS-CoV spike protein cDNA in our country.<br> | ||
+ | Since there was no patient of SARS, few researchers used it.<br> | ||
+ | We can get the cDNA by 200 dollars from this overseas website.<br> | ||
+ | :http://www.bcgsc.ca/project/sars/SARS/clones<br> | ||
+ | It is not easy to bring cDNA in here, so we should keep finding a better way.<br><br> | ||
+ | <span style=font-size:15px> <b> Sequence we used </b> </span><br> | ||
+ | 1. Cytokine Receptor | ||
+ | :1.1 ER Receptor | making | ||
+ | :1.2 HER2 Receptor | making | ||
+ | 2. JAK JAK.fasta | ||
+ | 3. STAT STAT.fasta | ||
+ | 4. GFP GFP.fasta | ||
+ | ER, HER2 Receptor signal peptide sequence is P07110[1-24]. <br>If we reverse-translate MKDRIPFAVNNITCVILLSLFCNA we can obtain outer membrane usher protein papC, Escherichia coli signal peptide<br> | ||
+ | :ATGAAGGACAGCATTCCATTCGCAGTAAACAACATCACGTGTGTCATACTGCTGTCCTTCTTCTGCAATGCG<br><br> | ||
+ | <span style=font-size:15px> <b> Anti-estrogen kappa/gamma chain </b> </span><br> | ||
+ | M.musculus mRNA for anti-estrogen receptor IG JS34/32 kappa chain V region | ||
+ | :http://www.ncbi.nlm.nih.gov/nuccore/51693 | ||
+ | |||
+ | M.musculus mRNA for anti-estrogen receptor IG JS34/32 gamma chain V region | ||
+ | :http://www.ncbi.nlm.nih.gov/nuccore/51690 | ||
+ | <br><br> | ||
+ | <span style=font-size:15px> <b> Anti-estrogen Receptor</b> </span><br> | ||
+ | |||
+ | Mouse Anti-Estrogen Receptor alpha Monoclonal Antibody, Unconjugated, Clone 33 | ||
+ | :http://www.bioreagents.com/products/printProductDetail/printProductDetails.cfm?catnbr=MA1-310 | ||
+ | |||
+ | Mouse Anti-Estrogen Receptor beta Monoclonal Antibody, Unconjugated, Clone 14C8 | ||
+ | :http://www.antibodies-online.com/antibody/137574/anti-estrogen+receptor+beta/ | ||
+ | |||
+ | Mouse Anti-Estrogen Receptor alpha Monoclonal Antibody, Unconjugated, Clone 6F11 | ||
+ | :http://www.genwaybio.com/product_info.php?products_id=70970 | ||
+ | <br><br> | ||
+ | |||
+ | <span style=font-size:15px> <b> Anti-estrogen Receptor</b> </span><br> | ||
+ | abcam makes this antigen, clone 1F3 Synthesize | ||
+ | :http://www.biocompare.com/ProductDetails/304675/Mouse-Anti-Estrogen-Receptor-alpha-Monoclonal-Antibody-Unconjugated-Clone-1F3.html | ||
+ | The other antibody | ||
+ | :http://www.biocompare.com/ProductListings/3194/Antibodies-Search.html?s=estrogen+receptor | ||
+ | NCBI information | ||
+ | :http://www.ncbi.nlm.nih.gov/nuccore/AF003711.1<br><br> | ||
+ | <span style=font-size:15px> <b> APRE element(STAT3 dimer binding site) sequence </b> </span><br> | ||
+ | :<b>Electrophoretic mobility shift assay (EMSA).</b><br> | ||
+ | The acute-phase response element (APRE; 5'-GCGCCTTCTGGGAAGATCCTTACGGGAATTCAG-3') double-stranded oligonucleotide probe for STAT3 binding was end-labelled using polynucleotide kinase and -32-ATP. The STAT3 EMSA was performed on equal amounts of whole total cell extracts from COS-1 cells prepared as described elsewhere. Binding reactions (20 l) contained 2 104 cpm of APRE probe in 10 mM Hepes at pH 7.8, 50 mM KCl, 1 mM EDTA, 5 mM MgCl2, 10% glycerol, 25 mM DTT and 2 g poly dI-dC. Competitive inhibition experiments were performed using a 20-fold molar excess of unlabeled oligonucleotides: WT (indicated above) and mutant (5'-GCGCCTTCTGGGCCTATCCTTACGGGCCGTCAG-3'). Supershift assay was performed by addition of antibodies to the Flag epitope. Binding reaction mixture was incubated at room temperature for 30 min. STAT3–APRE complexes were resolved on 5% non-denaturing polyacrylamide gels, and radiolabelled bands were visualized by autoradiography.<br> | ||
+ | :http://www.nature.com/ncb/journal/v6/n6/full/ncb1138.html <br><br> | ||
+ | <span style=font-size:15px> <b> Homo sapiens Janus kinase 1 (JAK1) as transfection </b> </span><br> | ||
+ | |||
+ | ::<b>OriGene ORF sequence for NM_002227 edited</b> | ||
+ | :ATGGCTTTCTGTGCTAAAATGAGGAGCTCCAAGAAGACTGAGGTGAACCTGGAGGCCCCT<br> | ||
+ | :GAGCCAGGGGTGGAAGTGATCTTCTATCTGTCGGACAGGGAGCCCCTCCGGCTGGGCAGT<br> | ||
+ | :GGAGAGTACACAGCAGAGGAACTGTGCATCAGGGCTGCACAGGCATGCCGTATCTCTCCT<br> | ||
+ | :CTTTGTCACAACCTCTTTGCCCTGTATGACGAGAACACCAAGCTCTGGTATGCTCCAAAT<br> | ||
+ | :CGCACCATCACCGTTGATGACAAGATGTCCCTCCGGCTCCACTACCGGATGAGGTTCTAT<br> | ||
+ | :TTCACCAATTGGCATGGAACCAACGACAATGAGCAGTCAGTGTGGCGTCATTCTCCAAAG<br> | ||
+ | :AAGCAGAAAAATGGCTACGAGAAAAAAAAGATTCCAGATGCAACCCCTCTCCTTGATGCC<br> | ||
+ | :AGCTCACTGGAGTATCTGTTTGCTCAGGGACAGTATGATTTGGTGAAATGCCTGGCTCCT<br> | ||
+ | :ATTCGAGACCCCAAGACCGAGCAGGATGGACATGATATTGAGAACGAGTGTCTAGGGATG<br> | ||
+ | :GCTGTCCTGGCCATCTCACACTATGCCATGATGAAGAAGATGCAGTTGCCAGAACTGCCC<br> | ||
+ | :AAGGACATCAGCTACAAGCGATATATTCCAGAAACATTGAATAAGTCCATCAGACAGAGG<br> | ||
+ | :AACCTTCTCACCAGGATGCGGATAAATAATGTTTTCAAGGATTTCCTAAAGGAATTTAAC<br> | ||
+ | :AACAAGACCATTTGTGACAGCAGCGTGTCCACGCATGACCTGAAGGTGAAATACTTGGCT<br> | ||
+ | :ACCTTGGAAACTTTGACAAAACATTACGGTGCTGAAATATTTGAGACTTCCATGTTACTG<br> | ||
+ | :ATTTCATCAGAAAATGAGATGAATTGGTTTCATTCGAATGACGGTGGAAACGTTCTCTAC<br> | ||
+ | :TACGAAGTGATGGTGACTGGGAATCTTGGAATCCAGTGGAGGCATAAACCAAATGTTGTT<br> | ||
+ | :TCTGTTGAAAAGGAAAAAAATAAACTGAAGCGGAAAAAACTGGAAAATAAACACAAGAAG<br> | ||
+ | :GATGAGGAGAAAAACAAGATCCGGGAAGAGTGGAACAATTTTTCTTACTTCCCTGAAATC<br> | ||
+ | :ACTCACATTGTAATAAAGGAGTCTGTGGTCAGCATTAACAAGCAGGACAACAAGAAAATG<br> | ||
+ | :GAACTGAAGCTCTCTTCCCACGAGGAGGCCTTGTCCTTTGTGTCCCTGGTAGATGGCTAC<br> | ||
+ | :TTCCGGCTCACAGCAGATGCCCATCATTACCTCTGCACCGACGTGGCCCCCCCGTTGATC<br> | ||
+ | :GTCCACAACATACAGAATGGCTGTCATGGTCCAATCTGTACAGAATACGCCATCAATAAA<br> | ||
+ | :TTGCGGCAAGAAGGAAGCGAGGAGGGGATGTACGTGCTGAGGTGGAGCTGCACCGACTTT<br> | ||
+ | :GACAACATCCTCATGACCGTCACCTGCTTTGAGAAGTCTGAGCAGGTGCAGGGTGCCCAG<br> | ||
+ | :AAGCAGTTCAAGAACTTTCAGATCGAGGTGCAGAAGGGCCGCTACAGTCTGCACGGTTCG<br> | ||
+ | :GACCGCAGCTTCCCCAGCTTGGGAGACCTCATGAGCCACCTCAAGAAGCAGATCCTGCGC<br> | ||
+ | :ACGGATAACATCAGCTTCATGCTAAAACGCTGCTGCCAGCCCAAGCCCCGAGAAATCTCC<br> | ||
+ | :AACCTGCTGGTGGCTACTAAGAAAGCCCAGGAGTGGCAGCCCGTCTACCCCATGAGCCAG<br> | ||
+ | :CTGAGTTTCGATCGGATCCTCAAGAAGGATCTGGTGCAGGGCGAGCACCTTGGGAGAGGC<br> | ||
+ | :ACGAGAACACACATCTATTCTGGGACCCTGATGGATTACAAGGATGACGAAGGAACTTCT<br> | ||
+ | :GAAGAGAAGAAGATAAAAGTGATCCTCAAAGTCTTAGACCCCAGCCACAGGGATATTTCC<br> | ||
+ | :CTGGCCTTCTTCGAGGCAGCCAGCATGATGAGACAGGTCTCCCACAAACACATCGTGTAC<br> | ||
+ | :CTCTATGGCGTCTGTGTCCGCGACGTGGAGAATATCATGGTGGAAGAGTTTGTGGAAGGG<br> | ||
+ | :GGTCCTCTGGATCTCTTCATGCACCGGAAAAGCGATGTCCTTACCACACCATGGAAATTC<br> | ||
+ | :AAAGTTGCCAAACAGCTGGCCAGTGCCCTGAGCTACTTGGAGGATAAAGACCTGGTCCAT<br> | ||
+ | :GGAAATGTGTGTACTAAAAACCTCCTCCTGGCCCGTGAGGGCATCGACAGTGAGTGTGGC<br> | ||
+ | :CCATTCATCAAGCTCAGTGACCCCGGCATCCCCATTACGGTGCTGTCTAGGCAAGAATGC<br> | ||
+ | :ATTGAACGAATCCCATGGATTGCTCCTGAGTGTGTTGAGGACTCCAAGAACCTGAGTGTG<br> | ||
+ | :GCTGCTGACAAGTGGAGCTTTGGAACCACGCTCTGGGAAATCTGCTACAATGGCGAGATC<br> | ||
+ | :CCCTTGAAAGACAAGACGCTGATTGAGAAAGAGAGATTCTATGAAAGCCGGTGCAGGCCA<br> | ||
+ | :GTGACACCATCATGTAAGGAGCTGGCTGACCTCATGACCCGCTGCATGAACTATGACCCC<br> | ||
+ | :AATCAGAGGCCTTTCTTCCGAGCCATCATGAGAGACATTAATAAGCTTGAAGAGCAGAAT<br> | ||
+ | :CCAGATATTGTTTCAGAAAAAAAACCAGCAACTGAAGTGGACCCCACACATTTTGAAAAG<br> | ||
+ | :CGCTTCCTAAAGAGGATCCGTGACTTGGGAGAGGGCCACTTTGGGAAGGTTGAGCTCTGC<br> | ||
+ | :AGGTATGACCCCGAAGGGGACAATACAGGGGAGCAGGTGGCTGTTAAATCTCTGAAGCCT<br> | ||
+ | :GAGAGTGGAGGTAACCACATAGCTGATCTGAAAAAGGAAATCGAGATCTTAAGGAACCTC<br> | ||
+ | :TATCATGAGAACATTGTGAAGTACAAAGGAATCTGCACAGAAGACGGAGGAAATGGTATT<br> | ||
+ | :AAGCTCATCATGGAATTTCTGCCTTCGGGAAGCCTTAAGGAATATCTTCCAAAGAATAAG<br> | ||
+ | :AACAAAATAAACCTCAAACAGCAGCTAAAATATGCCGTTCAGATTTGTAAGGGGATGGAC<br> | ||
+ | :TATTTGGGTTCTCGGCAATACGTTCACCGGGACTTGGCAGCAAGAAATGTCCTTGTTGAG<br> | ||
+ | :AGTGAACACCAAGTGAAAATTGGAGACTTCGGTTTAACCAAAGCAATTGAAACCGATAAG<br> | ||
+ | :GAGTATTACACCGTCAAGGATGACCGGGACAGCCCTGTGTTTTGGTATGCTCCAGAATGT<br> | ||
+ | :TTAATGCAATCTAAATTTTATATTGCCTCTGACGTCTGGTCTTTTGGAGTCACTCTGCAT<br> | ||
+ | :GAGCTGCTGACTTACTGTGATTCAGATTCTAGTCCCATGGCTTTGTTCCTGAAAATGATA<br> | ||
+ | :GGCCCAACCCATGGCCAGATGACAGTCACAAGACTTGTGAATACGTTAAAAGAAGGAAAA<br> | ||
+ | :CGCCTGCCGTGCCCACCTAACTGTCCAGATGAGGTTTATCAACTTATGAGGAAATGCTGG<br> | ||
+ | :GAATTCCAACCATCCAATCGGACAAGCTTTCAGAACCTTATTGAAGGATTTGAAGCACTT<br> | ||
+ | :TTAAAATAA<br><br> | ||
</td> | </td> | ||
Line 65: | Line 173: | ||
</tr> | </tr> | ||
+ | </table> | ||
+ | |||
+ | |||
+ | </td> | ||
+ | </tr> | ||
</table> | </table> |
Latest revision as of 05:02, 17 October 2010
|