Team:KAIST-Korea/Notebook/Memo/Data

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== <span style=font-size:20px> <b> Data </b> </span> ==
 
-
Brain storming. <br><br>
 
-
<span style=font-size:15px> <b> Idea 1 </b> </span>
+
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<td>
 +
== <span style=font-size:20px> <b> Data </b> </span> ==
<br>
<br>
-
leeches prevent blood to be harden. And there are product using vampire bat. If we use bacteria this as anti coagulant. We can localize this using bacteria.
+
<span style=font-size:15px> <b> pDRAW32 </b> </span>
-
<br><br>
+
-
<span style=font-size:15px> <b> Idea 2 </b> </span>
+
<br>
<br>
-
Using bacteria we could make a carrier that have chemicals that can cure cancer with targeting.  
+
pDRAW32 is a program which draws plasmid or linear gene, and it scans restriction enzyme site of its own DB.<br>
 +
You can see RFLP pattern.<br>
 +
You can download it at http://www.acaclone.com/
<br><br>
<br><br>
-
<span style=font-size:15px> <b> Idea 3 </b> </span>
+
<span style=font-size:15px> <b> Protein information for virus-inspired Drug delivery system. </b> </span>
<br>
<br>
-
Using magnetosome we could make a system that can detect magnetic field like other iGEM team detected toxicity.
+
HNMT
-
problem
+
Entrez ID : 3176
-
This bacteria is reacting passively to the magnetic field. Therefore it is not appropriate for detection.
+
:related mRNA or protein sequence
 +
 
 +
NM_001024074.2 → NP_001019245.1
 +
:histamine N-methyltransferase isoform 2
 +
 
 +
NM_001024075.1 → NP_001019246.1
 +
:histamine N-methyltransferase isoform 3
 +
 
 +
NM_006895.2 → NP_008826.1
 +
:histamine N-methyltransferase isoform 1
 +
(3 kinds of isoform is obtained by alternative splicing)
 +
 
 +
Human Coronaviral Spike protein.
 +
:NL63 strain
 +
Nucleotide sequence is in
 +
:http://www.ncbi.nlm.nih.gov/nuccore/49169782/?from=20472&to=24542&report=fasta (Gene ID : 2943499)
 +
protein sequence is in
 +
:http://www.ncbi.nlm.nih.gov/protein/45655909?report=genpept
 +
 
 +
SARS starin
 +
nucleotide sequence is in
 +
:http://www.ncbi.nlm.nih.gov/nuccore/30271926/?from=21492&to=25259&report=fasta (Gene ID : 1489668)
 +
protein sequence is in
 +
:http://www.ncbi.nlm.nih.gov/protein/29836496?report=genpept
<br><br>
<br><br>
-
<span style=font-size:15px> <b> Idea 4 </b> </span>
+
<span style=font-size:15px> <b> SARS-CoV Spike Protein </b> </span><br>
-
<br>
+
It seems very hard to get SARS-CoV spike protein cDNA in our country.<br>
-
Want to make a oscillator. There are memory network that remembers. And with that we can combine this with telomere. We can later use this protocol.
+
Since there was no patient of SARS, few researchers used it.<br>
 +
We can get the cDNA by 200 dollars from this overseas website.<br>
 +
:http://www.bcgsc.ca/project/sars/SARS/clones<br>
 +
It is not easy to bring cDNA in here, so we should keep finding a better way.<br><br>
 +
 
 +
<span style=font-size:15px> <b> Sequence we used </b> </span><br>
 +
1. Cytokine Receptor
 +
:1.1 ER Receptor | making
 +
:1.2 HER2 Receptor | making
 +
2. JAK JAK.fasta
 +
3. STAT STAT.fasta
 +
4. GFP GFP.fasta
 +
 
 +
ER, HER2 Receptor signal peptide sequence is P07110[1-24]. <br>If we reverse-translate MKDRIPFAVNNITCVILLSLFCNA we can obtain outer membrane usher protein papC, Escherichia coli signal peptide<br>
 +
:ATGAAGGACAGCATTCCATTCGCAGTAAACAACATCACGTGTGTCATACTGCTGTCCTTCTTCTGCAATGCG<br><br>
 +
<span style=font-size:15px> <b> Anti-estrogen kappa/gamma chain </b> </span><br>
 +
 
 +
M.musculus mRNA for anti-estrogen receptor IG JS34/32 kappa chain V region
 +
:http://www.ncbi.nlm.nih.gov/nuccore/51693
 +
 
 +
M.musculus mRNA for anti-estrogen receptor IG JS34/32 gamma chain V region
 +
:http://www.ncbi.nlm.nih.gov/nuccore/51690
<br><br>
<br><br>
-
<span style=font-size:15px> <b> Idea 5 </b> </span>
+
<span style=font-size:15px> <b> Anti-estrogen Receptor</b> </span><br>
-
<br>
+
-
Using vibration maybe we can make bacteria make sound.
+
-
<br><br>
+
-
<span style=font-size:15px> <b> Idea 6 </b> </span>
+
-
<br>
+
-
If we can make a NAND gate, we can make a calculator. Maybe we can make a compare system. And maybe we can use this for comparing concentration difference. And in case of NAND gate maybe we can in one cell system. If we make this in one cell then we can apply on other similar systems.
+
-
<br><br>
+
-
<span style=font-size:15px> <b> Idea 7 </b> </span>
+
-
<br>
+
-
We can put antigen in mosquitoes saliva And naturally we put antigens in people to get tolerance to malaria. Since malaria mosquitoes are active at night we can use mosquitoes that are active at day.
+
-
problem
+
-
It could make a problem if accidently inject too much antigens.
+
-
<br><br>
+
-
<span style=font-size:15px> <b> Idea 8 </b> </span>
+
-
<br>
+
-
In case of DDS packing is the problem. And we can use vesicle system for packing.
+
-
<br><br>
+
-
<span style=font-size:15px> <b> Idea 9 </b> </span>
+
-
<br>
+
-
Using Na+ pump maybe we can make electricity. Even there is a similar study that is exists. There are microorganism battery which circulate electron. If we use this system in bacteria we might make battery.
+
-
<br><br>
+
-
<span style=font-size:15px> <b> Idea 10 </b> </span>
+
-
<br>
+
-
RBC can trap virus because virus can't replicate in RBC. But this system need to use fake cell only to delay. And only has probability to trap. Therefore it is not perfect system. Maybe Since it is making delay, we can use it at rabies because its infection timing is faster than recovering timing.
+
-
<br><br>
+
-
<span style=font-size:15px> <b> Idea 11 </b> </span>
+
-
<br>
+
-
There are many steps in mammalian cell replication if we make a mammalian cell replicatable in vitro, it would be very convenient in laboratory life.
+
-
<br><br><br>
+
-
<span style=font-size:17px> <b> Idea Sum-up </b> </span>
+
-
<br>
+
-
Maybe we can work on mammalian cell replication tool or DDS that works on respiratory system. So we decided to look for that information.
+
 +
Mouse Anti-Estrogen Receptor alpha Monoclonal Antibody, Unconjugated, Clone 33
 +
:http://www.bioreagents.com/products/printProductDetail/printProductDetails.cfm?catnbr=MA1-310
 +
 +
Mouse Anti-Estrogen Receptor beta Monoclonal Antibody, Unconjugated, Clone 14C8
 +
:http://www.antibodies-online.com/antibody/137574/anti-estrogen+receptor+beta/
 +
 +
Mouse Anti-Estrogen Receptor alpha Monoclonal Antibody, Unconjugated, Clone 6F11
 +
:http://www.genwaybio.com/product_info.php?products_id=70970
 +
<br><br>
 +
<span style=font-size:15px> <b> Anti-estrogen Receptor</b> </span><br>
 +
abcam makes this antigen, clone 1F3 Synthesize
 +
:http://www.biocompare.com/ProductDetails/304675/Mouse-Anti-Estrogen-Receptor-alpha-Monoclonal-Antibody-Unconjugated-Clone-1F3.html
 +
The other antibody
 +
:http://www.biocompare.com/ProductListings/3194/Antibodies-Search.html?s=estrogen+receptor
 +
NCBI information
 +
:http://www.ncbi.nlm.nih.gov/nuccore/AF003711.1<br><br>
 +
<span style=font-size:15px> <b> APRE element(STAT3 dimer binding site) sequence  </b> </span><br>
 +
:<b>Electrophoretic mobility shift assay (EMSA).</b><br>
 +
The acute-phase response element (APRE; 5'-GCGCCTTCTGGGAAGATCCTTACGGGAATTCAG-3') double-stranded oligonucleotide probe for STAT3 binding was end-labelled using polynucleotide kinase and -32-ATP. The STAT3 EMSA was performed on equal amounts of whole total cell extracts from COS-1 cells prepared as described elsewhere. Binding reactions (20 l) contained 2  104 cpm of APRE probe in 10 mM Hepes at pH 7.8, 50 mM KCl, 1 mM EDTA, 5 mM MgCl2, 10% glycerol, 25 mM DTT and 2 g poly dI-dC. Competitive inhibition experiments were performed using a 20-fold molar excess of unlabeled oligonucleotides: WT (indicated above) and mutant (5'-GCGCCTTCTGGGCCTATCCTTACGGGCCGTCAG-3'). Supershift assay was performed by addition of antibodies to the Flag epitope. Binding reaction mixture was incubated at room temperature for 30 min. STAT3–APRE complexes were resolved on 5% non-denaturing polyacrylamide gels, and radiolabelled bands were visualized by autoradiography.<br>
 +
:http://www.nature.com/ncb/journal/v6/n6/full/ncb1138.html <br><br>
 +
<span style=font-size:15px> <b> Homo sapiens Janus kinase 1 (JAK1) as transfection </b> </span><br>
 +
::<b>OriGene ORF sequence for NM_002227 edited</b>
 +
:ATGGCTTTCTGTGCTAAAATGAGGAGCTCCAAGAAGACTGAGGTGAACCTGGAGGCCCCT<br>
 +
:GAGCCAGGGGTGGAAGTGATCTTCTATCTGTCGGACAGGGAGCCCCTCCGGCTGGGCAGT<br>
 +
:GGAGAGTACACAGCAGAGGAACTGTGCATCAGGGCTGCACAGGCATGCCGTATCTCTCCT<br>
 +
:CTTTGTCACAACCTCTTTGCCCTGTATGACGAGAACACCAAGCTCTGGTATGCTCCAAAT<br>
 +
:CGCACCATCACCGTTGATGACAAGATGTCCCTCCGGCTCCACTACCGGATGAGGTTCTAT<br>
 +
:TTCACCAATTGGCATGGAACCAACGACAATGAGCAGTCAGTGTGGCGTCATTCTCCAAAG<br>
 +
:AAGCAGAAAAATGGCTACGAGAAAAAAAAGATTCCAGATGCAACCCCTCTCCTTGATGCC<br>
 +
:AGCTCACTGGAGTATCTGTTTGCTCAGGGACAGTATGATTTGGTGAAATGCCTGGCTCCT<br>
 +
:ATTCGAGACCCCAAGACCGAGCAGGATGGACATGATATTGAGAACGAGTGTCTAGGGATG<br>
 +
:GCTGTCCTGGCCATCTCACACTATGCCATGATGAAGAAGATGCAGTTGCCAGAACTGCCC<br>
 +
:AAGGACATCAGCTACAAGCGATATATTCCAGAAACATTGAATAAGTCCATCAGACAGAGG<br>
 +
:AACCTTCTCACCAGGATGCGGATAAATAATGTTTTCAAGGATTTCCTAAAGGAATTTAAC<br>
 +
:AACAAGACCATTTGTGACAGCAGCGTGTCCACGCATGACCTGAAGGTGAAATACTTGGCT<br>
 +
:ACCTTGGAAACTTTGACAAAACATTACGGTGCTGAAATATTTGAGACTTCCATGTTACTG<br>
 +
:ATTTCATCAGAAAATGAGATGAATTGGTTTCATTCGAATGACGGTGGAAACGTTCTCTAC<br>
 +
:TACGAAGTGATGGTGACTGGGAATCTTGGAATCCAGTGGAGGCATAAACCAAATGTTGTT<br>
 +
:TCTGTTGAAAAGGAAAAAAATAAACTGAAGCGGAAAAAACTGGAAAATAAACACAAGAAG<br>
 +
:GATGAGGAGAAAAACAAGATCCGGGAAGAGTGGAACAATTTTTCTTACTTCCCTGAAATC<br>
 +
:ACTCACATTGTAATAAAGGAGTCTGTGGTCAGCATTAACAAGCAGGACAACAAGAAAATG<br>
 +
:GAACTGAAGCTCTCTTCCCACGAGGAGGCCTTGTCCTTTGTGTCCCTGGTAGATGGCTAC<br>
 +
:TTCCGGCTCACAGCAGATGCCCATCATTACCTCTGCACCGACGTGGCCCCCCCGTTGATC<br>
 +
:GTCCACAACATACAGAATGGCTGTCATGGTCCAATCTGTACAGAATACGCCATCAATAAA<br>
 +
:TTGCGGCAAGAAGGAAGCGAGGAGGGGATGTACGTGCTGAGGTGGAGCTGCACCGACTTT<br>
 +
:GACAACATCCTCATGACCGTCACCTGCTTTGAGAAGTCTGAGCAGGTGCAGGGTGCCCAG<br>
 +
:AAGCAGTTCAAGAACTTTCAGATCGAGGTGCAGAAGGGCCGCTACAGTCTGCACGGTTCG<br>
 +
:GACCGCAGCTTCCCCAGCTTGGGAGACCTCATGAGCCACCTCAAGAAGCAGATCCTGCGC<br>
 +
:ACGGATAACATCAGCTTCATGCTAAAACGCTGCTGCCAGCCCAAGCCCCGAGAAATCTCC<br>
 +
:AACCTGCTGGTGGCTACTAAGAAAGCCCAGGAGTGGCAGCCCGTCTACCCCATGAGCCAG<br>
 +
:CTGAGTTTCGATCGGATCCTCAAGAAGGATCTGGTGCAGGGCGAGCACCTTGGGAGAGGC<br>
 +
:ACGAGAACACACATCTATTCTGGGACCCTGATGGATTACAAGGATGACGAAGGAACTTCT<br>
 +
:GAAGAGAAGAAGATAAAAGTGATCCTCAAAGTCTTAGACCCCAGCCACAGGGATATTTCC<br>
 +
:CTGGCCTTCTTCGAGGCAGCCAGCATGATGAGACAGGTCTCCCACAAACACATCGTGTAC<br>
 +
:CTCTATGGCGTCTGTGTCCGCGACGTGGAGAATATCATGGTGGAAGAGTTTGTGGAAGGG<br>
 +
:GGTCCTCTGGATCTCTTCATGCACCGGAAAAGCGATGTCCTTACCACACCATGGAAATTC<br>
 +
:AAAGTTGCCAAACAGCTGGCCAGTGCCCTGAGCTACTTGGAGGATAAAGACCTGGTCCAT<br>
 +
:GGAAATGTGTGTACTAAAAACCTCCTCCTGGCCCGTGAGGGCATCGACAGTGAGTGTGGC<br>
 +
:CCATTCATCAAGCTCAGTGACCCCGGCATCCCCATTACGGTGCTGTCTAGGCAAGAATGC<br>
 +
:ATTGAACGAATCCCATGGATTGCTCCTGAGTGTGTTGAGGACTCCAAGAACCTGAGTGTG<br>
 +
:GCTGCTGACAAGTGGAGCTTTGGAACCACGCTCTGGGAAATCTGCTACAATGGCGAGATC<br>
 +
:CCCTTGAAAGACAAGACGCTGATTGAGAAAGAGAGATTCTATGAAAGCCGGTGCAGGCCA<br>
 +
:GTGACACCATCATGTAAGGAGCTGGCTGACCTCATGACCCGCTGCATGAACTATGACCCC<br>
 +
:AATCAGAGGCCTTTCTTCCGAGCCATCATGAGAGACATTAATAAGCTTGAAGAGCAGAAT<br>
 +
:CCAGATATTGTTTCAGAAAAAAAACCAGCAACTGAAGTGGACCCCACACATTTTGAAAAG<br>
 +
:CGCTTCCTAAAGAGGATCCGTGACTTGGGAGAGGGCCACTTTGGGAAGGTTGAGCTCTGC<br>
 +
:AGGTATGACCCCGAAGGGGACAATACAGGGGAGCAGGTGGCTGTTAAATCTCTGAAGCCT<br>
 +
:GAGAGTGGAGGTAACCACATAGCTGATCTGAAAAAGGAAATCGAGATCTTAAGGAACCTC<br>
 +
:TATCATGAGAACATTGTGAAGTACAAAGGAATCTGCACAGAAGACGGAGGAAATGGTATT<br>
 +
:AAGCTCATCATGGAATTTCTGCCTTCGGGAAGCCTTAAGGAATATCTTCCAAAGAATAAG<br>
 +
:AACAAAATAAACCTCAAACAGCAGCTAAAATATGCCGTTCAGATTTGTAAGGGGATGGAC<br>
 +
:TATTTGGGTTCTCGGCAATACGTTCACCGGGACTTGGCAGCAAGAAATGTCCTTGTTGAG<br>
 +
:AGTGAACACCAAGTGAAAATTGGAGACTTCGGTTTAACCAAAGCAATTGAAACCGATAAG<br>
 +
:GAGTATTACACCGTCAAGGATGACCGGGACAGCCCTGTGTTTTGGTATGCTCCAGAATGT<br>
 +
:TTAATGCAATCTAAATTTTATATTGCCTCTGACGTCTGGTCTTTTGGAGTCACTCTGCAT<br>
 +
:GAGCTGCTGACTTACTGTGATTCAGATTCTAGTCCCATGGCTTTGTTCCTGAAAATGATA<br>
 +
:GGCCCAACCCATGGCCAGATGACAGTCACAAGACTTGTGAATACGTTAAAAGAAGGAAAA<br>
 +
:CGCCTGCCGTGCCCACCTAACTGTCCAGATGAGGTTTATCAACTTATGAGGAAATGCTGG<br>
 +
:GAATTCCAACCATCCAATCGGACAAGCTTTCAGAACCTTATTGAAGGATTTGAAGCACTT<br>
 +
:TTAAAATAA<br><br>
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Latest revision as of 05:02, 17 October 2010

 

Data


pDRAW32
pDRAW32 is a program which draws plasmid or linear gene, and it scans restriction enzyme site of its own DB.
You can see RFLP pattern.
You can download it at http://www.acaclone.com/

Protein information for virus-inspired Drug delivery system.
HNMT Entrez ID : 3176

related mRNA or protein sequence

NM_001024074.2 → NP_001019245.1

histamine N-methyltransferase isoform 2

NM_001024075.1 → NP_001019246.1

histamine N-methyltransferase isoform 3

NM_006895.2 → NP_008826.1

histamine N-methyltransferase isoform 1

(3 kinds of isoform is obtained by alternative splicing)

Human Coronaviral Spike protein.

NL63 strain

Nucleotide sequence is in

http://www.ncbi.nlm.nih.gov/nuccore/49169782/?from=20472&to=24542&report=fasta (Gene ID : 2943499)

protein sequence is in

http://www.ncbi.nlm.nih.gov/protein/45655909?report=genpept

SARS starin nucleotide sequence is in

http://www.ncbi.nlm.nih.gov/nuccore/30271926/?from=21492&to=25259&report=fasta (Gene ID : 1489668)

protein sequence is in

http://www.ncbi.nlm.nih.gov/protein/29836496?report=genpept



SARS-CoV Spike Protein
It seems very hard to get SARS-CoV spike protein cDNA in our country.
Since there was no patient of SARS, few researchers used it.
We can get the cDNA by 200 dollars from this overseas website.

http://www.bcgsc.ca/project/sars/SARS/clones

It is not easy to bring cDNA in here, so we should keep finding a better way.

Sequence we used
1. Cytokine Receptor

1.1 ER Receptor | making
1.2 HER2 Receptor | making

2. JAK JAK.fasta 3. STAT STAT.fasta 4. GFP GFP.fasta

ER, HER2 Receptor signal peptide sequence is P07110[1-24].
If we reverse-translate MKDRIPFAVNNITCVILLSLFCNA we can obtain outer membrane usher protein papC, Escherichia coli signal peptide

ATGAAGGACAGCATTCCATTCGCAGTAAACAACATCACGTGTGTCATACTGCTGTCCTTCTTCTGCAATGCG

Anti-estrogen kappa/gamma chain

M.musculus mRNA for anti-estrogen receptor IG JS34/32 kappa chain V region

http://www.ncbi.nlm.nih.gov/nuccore/51693

M.musculus mRNA for anti-estrogen receptor IG JS34/32 gamma chain V region

http://www.ncbi.nlm.nih.gov/nuccore/51690



Anti-estrogen Receptor

Mouse Anti-Estrogen Receptor alpha Monoclonal Antibody, Unconjugated, Clone 33

http://www.bioreagents.com/products/printProductDetail/printProductDetails.cfm?catnbr=MA1-310

Mouse Anti-Estrogen Receptor beta Monoclonal Antibody, Unconjugated, Clone 14C8

http://www.antibodies-online.com/antibody/137574/anti-estrogen+receptor+beta/

Mouse Anti-Estrogen Receptor alpha Monoclonal Antibody, Unconjugated, Clone 6F11

http://www.genwaybio.com/product_info.php?products_id=70970



Anti-estrogen Receptor
abcam makes this antigen, clone 1F3 Synthesize

http://www.biocompare.com/ProductDetails/304675/Mouse-Anti-Estrogen-Receptor-alpha-Monoclonal-Antibody-Unconjugated-Clone-1F3.html

The other antibody

http://www.biocompare.com/ProductListings/3194/Antibodies-Search.html?s=estrogen+receptor

NCBI information

http://www.ncbi.nlm.nih.gov/nuccore/AF003711.1

APRE element(STAT3 dimer binding site) sequence

Electrophoretic mobility shift assay (EMSA).

The acute-phase response element (APRE; 5'-GCGCCTTCTGGGAAGATCCTTACGGGAATTCAG-3') double-stranded oligonucleotide probe for STAT3 binding was end-labelled using polynucleotide kinase and -32-ATP. The STAT3 EMSA was performed on equal amounts of whole total cell extracts from COS-1 cells prepared as described elsewhere. Binding reactions (20 l) contained 2 104 cpm of APRE probe in 10 mM Hepes at pH 7.8, 50 mM KCl, 1 mM EDTA, 5 mM MgCl2, 10% glycerol, 25 mM DTT and 2 g poly dI-dC. Competitive inhibition experiments were performed using a 20-fold molar excess of unlabeled oligonucleotides: WT (indicated above) and mutant (5'-GCGCCTTCTGGGCCTATCCTTACGGGCCGTCAG-3'). Supershift assay was performed by addition of antibodies to the Flag epitope. Binding reaction mixture was incubated at room temperature for 30 min. STAT3–APRE complexes were resolved on 5% non-denaturing polyacrylamide gels, and radiolabelled bands were visualized by autoradiography.

http://www.nature.com/ncb/journal/v6/n6/full/ncb1138.html

Homo sapiens Janus kinase 1 (JAK1) as transfection

OriGene ORF sequence for NM_002227 edited
ATGGCTTTCTGTGCTAAAATGAGGAGCTCCAAGAAGACTGAGGTGAACCTGGAGGCCCCT
GAGCCAGGGGTGGAAGTGATCTTCTATCTGTCGGACAGGGAGCCCCTCCGGCTGGGCAGT
GGAGAGTACACAGCAGAGGAACTGTGCATCAGGGCTGCACAGGCATGCCGTATCTCTCCT
CTTTGTCACAACCTCTTTGCCCTGTATGACGAGAACACCAAGCTCTGGTATGCTCCAAAT
CGCACCATCACCGTTGATGACAAGATGTCCCTCCGGCTCCACTACCGGATGAGGTTCTAT
TTCACCAATTGGCATGGAACCAACGACAATGAGCAGTCAGTGTGGCGTCATTCTCCAAAG
AAGCAGAAAAATGGCTACGAGAAAAAAAAGATTCCAGATGCAACCCCTCTCCTTGATGCC
AGCTCACTGGAGTATCTGTTTGCTCAGGGACAGTATGATTTGGTGAAATGCCTGGCTCCT
ATTCGAGACCCCAAGACCGAGCAGGATGGACATGATATTGAGAACGAGTGTCTAGGGATG
GCTGTCCTGGCCATCTCACACTATGCCATGATGAAGAAGATGCAGTTGCCAGAACTGCCC
AAGGACATCAGCTACAAGCGATATATTCCAGAAACATTGAATAAGTCCATCAGACAGAGG
AACCTTCTCACCAGGATGCGGATAAATAATGTTTTCAAGGATTTCCTAAAGGAATTTAAC
AACAAGACCATTTGTGACAGCAGCGTGTCCACGCATGACCTGAAGGTGAAATACTTGGCT
ACCTTGGAAACTTTGACAAAACATTACGGTGCTGAAATATTTGAGACTTCCATGTTACTG
ATTTCATCAGAAAATGAGATGAATTGGTTTCATTCGAATGACGGTGGAAACGTTCTCTAC
TACGAAGTGATGGTGACTGGGAATCTTGGAATCCAGTGGAGGCATAAACCAAATGTTGTT
TCTGTTGAAAAGGAAAAAAATAAACTGAAGCGGAAAAAACTGGAAAATAAACACAAGAAG
GATGAGGAGAAAAACAAGATCCGGGAAGAGTGGAACAATTTTTCTTACTTCCCTGAAATC
ACTCACATTGTAATAAAGGAGTCTGTGGTCAGCATTAACAAGCAGGACAACAAGAAAATG
GAACTGAAGCTCTCTTCCCACGAGGAGGCCTTGTCCTTTGTGTCCCTGGTAGATGGCTAC
TTCCGGCTCACAGCAGATGCCCATCATTACCTCTGCACCGACGTGGCCCCCCCGTTGATC
GTCCACAACATACAGAATGGCTGTCATGGTCCAATCTGTACAGAATACGCCATCAATAAA
TTGCGGCAAGAAGGAAGCGAGGAGGGGATGTACGTGCTGAGGTGGAGCTGCACCGACTTT
GACAACATCCTCATGACCGTCACCTGCTTTGAGAAGTCTGAGCAGGTGCAGGGTGCCCAG
AAGCAGTTCAAGAACTTTCAGATCGAGGTGCAGAAGGGCCGCTACAGTCTGCACGGTTCG
GACCGCAGCTTCCCCAGCTTGGGAGACCTCATGAGCCACCTCAAGAAGCAGATCCTGCGC
ACGGATAACATCAGCTTCATGCTAAAACGCTGCTGCCAGCCCAAGCCCCGAGAAATCTCC
AACCTGCTGGTGGCTACTAAGAAAGCCCAGGAGTGGCAGCCCGTCTACCCCATGAGCCAG
CTGAGTTTCGATCGGATCCTCAAGAAGGATCTGGTGCAGGGCGAGCACCTTGGGAGAGGC
ACGAGAACACACATCTATTCTGGGACCCTGATGGATTACAAGGATGACGAAGGAACTTCT
GAAGAGAAGAAGATAAAAGTGATCCTCAAAGTCTTAGACCCCAGCCACAGGGATATTTCC
CTGGCCTTCTTCGAGGCAGCCAGCATGATGAGACAGGTCTCCCACAAACACATCGTGTAC
CTCTATGGCGTCTGTGTCCGCGACGTGGAGAATATCATGGTGGAAGAGTTTGTGGAAGGG
GGTCCTCTGGATCTCTTCATGCACCGGAAAAGCGATGTCCTTACCACACCATGGAAATTC
AAAGTTGCCAAACAGCTGGCCAGTGCCCTGAGCTACTTGGAGGATAAAGACCTGGTCCAT
GGAAATGTGTGTACTAAAAACCTCCTCCTGGCCCGTGAGGGCATCGACAGTGAGTGTGGC
CCATTCATCAAGCTCAGTGACCCCGGCATCCCCATTACGGTGCTGTCTAGGCAAGAATGC
ATTGAACGAATCCCATGGATTGCTCCTGAGTGTGTTGAGGACTCCAAGAACCTGAGTGTG
GCTGCTGACAAGTGGAGCTTTGGAACCACGCTCTGGGAAATCTGCTACAATGGCGAGATC
CCCTTGAAAGACAAGACGCTGATTGAGAAAGAGAGATTCTATGAAAGCCGGTGCAGGCCA
GTGACACCATCATGTAAGGAGCTGGCTGACCTCATGACCCGCTGCATGAACTATGACCCC
AATCAGAGGCCTTTCTTCCGAGCCATCATGAGAGACATTAATAAGCTTGAAGAGCAGAAT
CCAGATATTGTTTCAGAAAAAAAACCAGCAACTGAAGTGGACCCCACACATTTTGAAAAG
CGCTTCCTAAAGAGGATCCGTGACTTGGGAGAGGGCCACTTTGGGAAGGTTGAGCTCTGC
AGGTATGACCCCGAAGGGGACAATACAGGGGAGCAGGTGGCTGTTAAATCTCTGAAGCCT
GAGAGTGGAGGTAACCACATAGCTGATCTGAAAAAGGAAATCGAGATCTTAAGGAACCTC
TATCATGAGAACATTGTGAAGTACAAAGGAATCTGCACAGAAGACGGAGGAAATGGTATT
AAGCTCATCATGGAATTTCTGCCTTCGGGAAGCCTTAAGGAATATCTTCCAAAGAATAAG
AACAAAATAAACCTCAAACAGCAGCTAAAATATGCCGTTCAGATTTGTAAGGGGATGGAC
TATTTGGGTTCTCGGCAATACGTTCACCGGGACTTGGCAGCAAGAAATGTCCTTGTTGAG
AGTGAACACCAAGTGAAAATTGGAGACTTCGGTTTAACCAAAGCAATTGAAACCGATAAG
GAGTATTACACCGTCAAGGATGACCGGGACAGCCCTGTGTTTTGGTATGCTCCAGAATGT
TTAATGCAATCTAAATTTTATATTGCCTCTGACGTCTGGTCTTTTGGAGTCACTCTGCAT
GAGCTGCTGACTTACTGTGATTCAGATTCTAGTCCCATGGCTTTGTTCCTGAAAATGATA
GGCCCAACCCATGGCCAGATGACAGTCACAAGACTTGTGAATACGTTAAAAGAAGGAAAA
CGCCTGCCGTGCCCACCTAACTGTCCAGATGAGGTTTATCAACTTATGAGGAAATGCTGG
GAATTCCAACCATCCAATCGGACAAGCTTTCAGAACCTTATTGAAGGATTTGAAGCACTT
TTAAAATAA


Retrieved from "http://2010.igem.org/Team:KAIST-Korea/Notebook/Memo/Data"