Team:IvyTech-South Bend/Notebook

From 2010.igem.org

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|{{ #calendar: title=Talk:Team:IvyTech-South_Bend |year=2010 | month=06 }}
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===IGEM Part #s and Uses===
 +
 
 +
BBa_T9002 – Producer Controler Device–Plate2 Well 9A
 +
 
 +
BBa_I732017 – LacZ with our GFP 1—Plate 2 Well 3K
 +
 
 +
BBa_F2620– LuxR with terminator– Plate 2 Well 6E
 +
 
 +
BBa_I13522 –Tet Prop with GFP—Plate 2 Well 8A
 +
 
 +
BBa_T13521 Red Flouro tet RFP—Plate 2 Well 6O
 +
 
 +
BBa_pSB1C3—Plasmid—Plate 1 Well 3A
 +
 
 +
BBa_pSB1T3—Tetracycline resistant plasmid—Plate 1 Well 7A
 +
 
 +
BBa_pSB1AT3--high copy number plasmid carrying ampicillin and tetracycline resistance--Plate 1 Well 13A
 +
 
 +
== 6/28/10 ==
 +
Preparing SOB
 +
 
 +
== 6/30/10 ==
 +
 
 +
Richard Lab:Electroporation of E. coli
 +
 
 +
== 7/28/10 ==
 +
 
 +
Usage and extraction
== 8/24/10 ==
== 8/24/10 ==
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Today we will be extracting DNA from out electroporated cells/T9002
Today we will be extracting DNA from out electroporated cells/T9002
 +
B-galactosidase Assay
== 9/16/10 ==
== 9/16/10 ==
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Today we will be doing a DNA Extraction from/of part T9002 for electroporation this will determine if we have a stable host for our beast this will also Work!
Today we will be doing a DNA Extraction from/of part T9002 for electroporation this will determine if we have a stable host for our beast this will also Work!
 +
 +
 +
== 10/18/10 ==
 +
 +
cutting enzymes
== 9/21/10 ==
== 9/21/10 ==
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Today we will electroporate  Agrobacterium with part T9002 using protocol from pg  22 – 23
+
Today we will electroporate  Agrobacterium with part T9002  
 +
 
 +
Electrophoresis
== 9/22/10 ==
== 9/22/10 ==
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== 9/28/10 ==
== 9/28/10 ==
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Today we will be picking 2 colonies from the Lacz, LuxR electroporated plates that were streaked on 9/22/10  
+
Today we will be picking 2 colonies from the Lacz, LuxR electroporated plates that were streaked on 9/22/10
 +
== 9/30/10==
 +
LacZ without GFP also with RBS and terminator
 +
 +
 +
==10/1/10==
 +
 +
electrophoresis
 +
 +
==10/5/10==
 +
 +
pTet GFP
 +
 +
==10/7/10==
 +
 +
Electroporating 2 parts with E-coli bacteria
 +
 +
==10/8/10==
 +
 +
 +
LB-Lennox Broth & Electroporation
 +
 +
==10/12/10==
 +
hold until Friday
 +
 +
==10/13/10==
 +
Discoveries!
 +
 +
==10/15/10==
 +
 +
Using openwetware.org protocol
 +
 +
B-galactosidase Assay
 +
 +
BBL Trypticase soy Broth and Agar
 +
 +
DPBS
 +
 +
== 10/19/10==
 +
Protocol-- Electroporation man for E-Coli
 +
== 10/21/10 ==
 +
High Efficiency Electrotransformation of E.coli
 +
 +
== 10/25/10 ==
 +
 +
DNA from BlueGuy
 +
 +
== 10/26/10 ==
 +
Final steps pack and ship
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|align="center"|[[Image:IvyTech-South_Bend_logo.png|200px|right|frame]]
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''Tell us more about your project.  Give us background.  Use this is the abstract of your project.  Be descriptive but concise (1-2 paragraphs)''
 
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|align="center"|[[Team:IvyTech-South_Bend | Team Example]]
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Latest revision as of 00:44, 28 October 2010

Discussion

Contents

Notebook

June
MTWTFSS
  1 2 3 4 5 6
7 8 9 10 11 12 13
14 15 16 17 18 19 20
21 22 23 24 25 26 27
28 29 30
July
MTWTFSS
      1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30 31
August
MTWTFSS
            1
2 3 4 5 6 7 8
9 10 11 12 13 14 15
16 17 18 19 20 21 22
23 24 25 26 27 28 29
30 31
September
MTWTFSS
    1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30
October
MTWTFSS
        1 2 3
4 5 6 7 8 9 10
11 12 13 14 15 16 17
18 19 20 21 22 23 24
25 26 27 28 29 30 31
November
MTWTFSS
1 2 3 4 5 6 7
8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30

IGEM Part #s and Uses

BBa_T9002 – Producer Controler Device–Plate2 Well 9A

BBa_I732017 – LacZ with our GFP 1—Plate 2 Well 3K

BBa_F2620– LuxR with terminator– Plate 2 Well 6E

BBa_I13522 –Tet Prop with GFP—Plate 2 Well 8A

BBa_T13521 Red Flouro tet RFP—Plate 2 Well 6O

BBa_pSB1C3—Plasmid—Plate 1 Well 3A

BBa_pSB1T3—Tetracycline resistant plasmid—Plate 1 Well 7A

BBa_pSB1AT3--high copy number plasmid carrying ampicillin and tetracycline resistance--Plate 1 Well 13A

6/28/10

Preparing SOB

6/30/10

Richard Lab:Electroporation of E. coli

7/28/10

Usage and extraction

8/24/10

Today we will be pouring new plates for streaking new transformed cells.

8/27/10

We will be running a gel to determine if our DNA sample was successfully electroplated.

8/27/10

Today we will be electroplating part KBBa_3131010

8/31/10

Results of transformation - the plates had growth but (no) distinct colony pattern.

8/31/10

After agar is dissolved, and allowed to cool, I will add Amp in different doses to specific plates. JH

9/1/10

Today the team will be making up the same plates as I made yesterday. Professor T. wants new Amp stock made to document how much is used into the stock.

9/2/10

Results from streaking –

9/2/10

Protocol For Making LB-Broth/Agar

9/3/10

Today I’m finishing making the LB/Agar from yesterday.

9/9/10

Due to conflict we will be changing from E Coli to yeast.

9/10/10

Lux Casette Right Promoter BBa_I1051

9/14/10

Today we found growth from our Electroporated E – Coli parts

9/15/10

Today we will be extracting DNA from out electroporated cells/T9002 B-galactosidase Assay

9/16/10

Today we will wash

9/17/10

Today we will be doing a DNA Extraction from/of part T9002 for electroporation this will determine if we have a stable host for our beast this will also Work!


10/18/10

cutting enzymes

9/21/10

Today we will electroporate Agrobacterium with part T9002

Electrophoresis

9/22/10

Today we have growth from electroporation done yesterday we are going to test by hitting with (AHL) the 20 mL grew not 5 mL sample

9/22/10

The only one that should glow should be (C)/(3) E – Coli w/transformed part in LB For Supernate of A.Tumafaciens

9/23/10

Today I have been setting up for the trial of our part T9002 inside agrobacteria to see if in the presence of AHL it floureces.

9/24/10

Today I’m testing absorbance of our trials @ 395nm

9/28/10

Today we will be picking 2 colonies from the Lacz, LuxR electroporated plates that were streaked on 9/22/10

9/30/10

LacZ without GFP also with RBS and terminator


10/1/10

electrophoresis

10/5/10

pTet GFP

10/7/10

Electroporating 2 parts with E-coli bacteria

10/8/10

LB-Lennox Broth & Electroporation

10/12/10

hold until Friday

10/13/10

Discoveries!

10/15/10

Using openwetware.org protocol

B-galactosidase Assay

BBL Trypticase soy Broth and Agar

DPBS

10/19/10

Protocol-- Electroporation man for E-Coli

10/21/10

High Efficiency Electrotransformation of E.coli

10/25/10

DNA from BlueGuy

10/26/10

Final steps pack and ship


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