Team:Imperial College London/Templates/ModuleHeader
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- | | style="font-size:1em;background-color:#ea8828;width:300px;border:20px solid #ea8828;border-top:0px;"|We decided to design a new mechanism for parasite detection - by using the proteases they release. A novel protein bound to the cell surface, with a | + | | style="font-size:1em;background-color:#ea8828;width:300px;border:20px solid #ea8828;border-top:0px;"|We decided to design a new mechanism for parasite detection - by using the proteases they release. A novel protein bound to the cell surface, with a signaling peptide attached via a protease cleavage site. When the protease comes along, the signal peptide is released, allowing it to activate our signaling module. |
| style="font-size:1em;background-color:#728175;width:300px;border:20px solid #728175;border-top:0px;" valign="top"| To transduce the signal we used a quorum sensing system of a gram positive bacterium. The two component signal transduction system taken from ''S. pneumoniae'' transfers our peptide signal into the cell, activating the fast response module. | | style="font-size:1em;background-color:#728175;width:300px;border:20px solid #728175;border-top:0px;" valign="top"| To transduce the signal we used a quorum sensing system of a gram positive bacterium. The two component signal transduction system taken from ''S. pneumoniae'' transfers our peptide signal into the cell, activating the fast response module. | ||
- | | style="font-size:1em;background-color:#e33333;width:300px;border:20px solid #e33333;border-top:0px;" valign="top"| Our fast response mechanism is based around using | + | | style="font-size:1em;background-color:#e33333;width:300px;border:20px solid #e33333;border-top:0px;" valign="top"| Our fast response mechanism is based around using two enzymatic amplification steps acting upon a deactivated enzyme and a presynthesised substrate. This greatly reduces the time required for producing a recognisable output, enabling useful field testing kits. |
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Revision as of 18:42, 27 October 2010
Detection Module | Signaling Module | Fast Response Module |
We decided to design a new mechanism for parasite detection - by using the proteases they release. A novel protein bound to the cell surface, with a signaling peptide attached via a protease cleavage site. When the protease comes along, the signal peptide is released, allowing it to activate our signaling module. | To transduce the signal we used a quorum sensing system of a gram positive bacterium. The two component signal transduction system taken from S. pneumoniae transfers our peptide signal into the cell, activating the fast response module. | Our fast response mechanism is based around using two enzymatic amplification steps acting upon a deactivated enzyme and a presynthesised substrate. This greatly reduces the time required for producing a recognisable output, enabling useful field testing kits. |