Team:Imperial College London/Media/Pictures

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Latest revision as of 02:38, 28 October 2010

Department of Bioengineering

Division of Molecular Biosciences

Media	Videos \| Pictures
Want to know what we've been up to this summer? Need to know how to structure your Synthetic Biology School Workshop? Or do you just want to check out some pictures of our project? No worries, just sit back and relax! We've compiled a whole host of videos and images to give you a taste of what we've been up to.

Logos

Our Imperial College iGEM Logo

PARASIGHT

Another really cool logo

The Team

The team with advisors Chris and Tom

The Imperial College iGEM Team

Some team members with SynBio icon Jay Keasling!!!

Maddie and a bit of Kirill in labs

Maddie in labs again

Nick

Maddie in labs... AGAIN

Kirill, Maddie and Nick

Nick (posing?)

Piotr focusing, Anita not so much!

Piotr and Anita

Maddie and Kirill

Anita and Ben

Nick knows ONE song on the guitar! Wow

Ania, Kyasha and Florian

The team and some of the advisors at Eastside.

Kyasha, Maddie and Harriet

Some of the team members at Eastside!

The team at the Human Practices Panel. Don't we look excited???

Our lovely advisors at the Human Practices Panel.

Some members of the team

Flo and Harriet

Susanna criticising our ideas?

Harriet and Wolf

Flo =)

Our advisors

Ben...

Brainstorming

Fv-fragment guided input module

Ideas on parasite detection

Nick's idea on cell population control

Nick's idea...

Wet Lab

Surface Protein

Surface Protein Team Logo

Assembly Strategy for LytC

Gel confirming that DNA (pSB1C3 and pSB1AK3 with BOO14) has been digested properly by PstI and EcoRI.

Gel of PCR confirming pSB1C3 has been amplified.

Picture estimating the concentration of purified BOO14 and pSB1C3.

Colony PCR to detect sucessfully transformed bacteria.

3 PCR reactions to determine the most effective protocol to amplify the CWB.

Gel of a diagnostic digest of the vector confirming that the terminator has been inserted.

Isolation of the promoter pVEG by gel purification.

Determining the ratio of pSB1C3 to pVEG for ligation.

Determining the ratio of lytC to pSB1C3 for ligation.

Vectors

Vectors Team Logo

Assembly strategy for the AmyE vector

Assembly strategy for the PyrD vector

K14002 and oligo assembly

K143070 and K11064 assembly step

LacI Testing Vector

XylE

XylE Team Logo

Team XylE

The J23101 gene in a biobrick vector containg RFP gene.

Midi-prep XylE digestion with xbaI and PstI

Gel analysis of XylE, J23101 promoter and pSB1C3 vector samples

Catechol assay (before)

Catechol assay (after) - Can you spot the difference?

Catechol assay on XylE-trasformed cells in a 96-well plate

First Successful Catechol Assay!

:)

Test confirming that yellow product of XylE enzmymatic reaction is leaks back from the cytosol into the solution.

The coloured pigment is transported out of the cells.

Spectra of XylE transformed E.coli after addition of catechol assay

Catechol Assay cuvette setup

Strategy

The Engineering Design Cycle

The full assembly strategy

Wolf's primers

Parts

BioBricks

ComE binding sites in the promoters of the comCDE and comAB operons

Detection Module

Dr Martha Betson explaining the Schistosoma lifecycle

Detection Module Construct

Surface Protein Construct

Detection Module Schematic

Signalling Module

Results of a BLAST search using ComE (Accession no. AAC44897)

Results of a BLAST search using BlpR (Accession no. CAC03515.1)

Signalling Module Construct

Signalling Module Pathway

Fast Response Module

Fast Response Module Construct

Fast Response Module Testing Construct

Human Practices

Human Practices panel: advisors

The team looks reeeeaaaalllly interested

Maddie's notes from the HP panel

Notes...

Should scientists be allowed to create artificial life?

Building prototypes informed our design

We wanted our kit to be as useful as possible

This is the first design...

A prototype of our dipstick concept!! So cool!!!

More pictures of our awesome prototype!!!

School Workshops

Brainstorming

Chameleon: Now you see me - now you don't

More brainstorming

...and more brainstorming

Ideas on regulation of Synthetic Biology

H'n'H sauce (Hot&Healthy) - yummy

More ideas on regulations of SynBio

Brainstorming again

iGEM <3

Check out the lens creating bacteria!

More ideas...

Happy Bacteria?!

Regulations...

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   <a href="https://static.igem.org/mediawiki/2010/5/56/IC_CS1.JPG"><img src="https://static.igem.org/mediawiki/2010/5/56/IC_CS1.JPG" width="200"/></a>
@@ Line 638: / Line 639: @@
 |}
 |<div ALIGN=CENTER>
-  {| style="background:#e7e7e7;text-align:center;font-family: helvetica, arial, sans-serif;color:#555555;margin- top:5px;" cellspacing="5";
+  {| style="width:204px;background:#e7e7e7;text-align:center;font-family: helvetica, arial, sans-serif;color:#555555;margin- top:5px;" cellspacing="5";
   |<html>
   <a href="https://static.igem.org/mediawiki/2010/5/5d/IC_CS2.JPG"><img src="https://static.igem.org/mediawiki/2010/5/5d/IC_CS2.JPG" width="200"/></a>
@@ Line 647: / Line 648: @@
 |}
 |<div ALIGN=CENTER>
-  {| style="background:#e7e7e7;text-align:center;font-family: helvetica, arial, sans-serif;color:#555555;margin- top:5px;" cellspacing="5";
+  {| style="width:204px;background:#e7e7e7;text-align:center;font-family: helvetica, arial, sans-serif;color:#555555;margin- top:5px;" cellspacing="5";
   |<html>
   <a href="https://static.igem.org/mediawiki/2010/c/c6/IC_200-600nm_spectr.jpg"><img src="https://static.igem.org/mediawiki/2010/c/c6/IC_200-600nm_spectr.jpg" width="200"/></a>
@@ Line 674: / Line 675: @@
 </div>
 |}
-|-
+|}
-|<html><h2>Strategy</h2></html>
+{| style="width:900px;background:#f5f5f5;text-align:justify;font-family: helvetica, arial, sans-serif;color:#555555;margin-top:5px;" cellspacing="20"
+|style="font-family: helvetica, arial, sans-serif;font-size:2em;color:#ea8828;"|Strategy
 |-
 |<div ALIGN=CENTER>
@@ Line 704: / Line 706: @@
 </div>
 |}
-|-
+|}
-|<html><h2>Parts</h2></html>
+{| style="width:900px;background:#f5f5f5;text-align:justify;font-family: helvetica, arial, sans-serif;color:#555555;margin-top:5px;" cellspacing="20"
+|style="font-family: helvetica, arial, sans-serif;font-size:2em;color:#ea8828;"|Parts
 |-
 |<div ALIGN=CENTER>
@@ Line 717: / Line 720: @@
 |}
 |<div ALIGN=CENTER>
-  {| style="background:#e7e7e7;text-align:center;font-family: helvetica, arial, sans-serif;color:#555555;margin- top:5px;" cellspacing="5";
+  {| style="width:204px;background:#e7e7e7;text-align:center;font-family: helvetica, arial, sans-serif;color:#555555;margin- top:5px;" cellspacing="5";
   |<html>
   <a href="https://static.igem.org/mediawiki/2010/b/b8/IC_ComE_binding.jpg"><img src="https://static.igem.org/mediawiki/2010/b/b8/IC_ComE_binding.jpg" width="200"/></a>
@@ Line 725: / Line 728: @@
 </div>
 |}
-|-
+|}
-|<html><h2>Detection Module</h2></html>
+{| style="width:900px;background:#f5f5f5;text-align:justify;font-family: helvetica, arial, sans-serif;color:#555555;margin-top:5px;" cellspacing="20"
+|style="font-family: helvetica, arial, sans-serif;font-size:2em;color:#ea8828;"|Detection Module
 |-
 |<div ALIGN=CENTER>
-  {| style="background:#e7e7e7;text-align:center;font-family: helvetica, arial, sans-serif;color:#555555;margin- top:5px;" cellspacing="5";
+  {| style="width:204px;background:#e7e7e7;text-align:center;font-family: helvetica, arial, sans-serif;color:#555555;margin- top:5px;" cellspacing="5";
   |<html>
   <a href="https://static.igem.org/mediawiki/2010/3/34/Jd.jpg"><img src="https://static.igem.org/mediawiki/2010/3/34/Jd.jpg" width="200"/></a>
@@ Line 783: / Line 787: @@
 </div>
 |}
-|-
+|}
-|<html><h2>Signalling Module</h2></html>
+{| style="width:900px;background:#f5f5f5;text-align:justify;font-family: helvetica, arial, sans-serif;color:#555555;margin-top:5px;" cellspacing="20"
+|style="font-family: helvetica, arial, sans-serif;font-size:2em;color:#ea8828;"|Signalling Module
 |-
 |<div ALIGN=CENTER>
-  {| style="background:#e7e7e7;text-align:center;font-family: helvetica, arial, sans-serif;color:#555555;margin- top:5px;" cellspacing="5";
+  {| style="width:204px;background:#e7e7e7;text-align:center;font-family: helvetica, arial, sans-serif;color:#555555;margin- top:5px;" cellspacing="5";
   |<html>
   <a href="https://static.igem.org/mediawiki/2010/6/6b/IC_Aac_blast.png"><img src="https://static.igem.org/mediawiki/2010/6/6b/IC_Aac_blast.png" width="200"/></a>
@@ Line 796: / Line 801: @@
 |}
 |<div ALIGN=CENTER>
-  {| style="background:#e7e7e7;text-align:center;font-family: helvetica, arial, sans-serif;color:#555555;margin- top:5px;" cellspacing="5";
+  {| style="width:204px;background:#e7e7e7;text-align:center;font-family: helvetica, arial, sans-serif;color:#555555;margin- top:5px;" cellspacing="5";
   |<html>
   <a href="https://static.igem.org/mediawiki/2010/2/27/IC_Cac_blast.png"><img src="https://static.igem.org/mediawiki/2010/2/27/IC_Cac_blast.png" width="200"/></a>
@@ Line 935: / Line 940: @@
 </div>
 |}
-|-
+|}
-|<html><h2>Fast Response Module</h2></html>
+{| style="width:900px;background:#f5f5f5;text-align:justify;font-family: helvetica, arial, sans-serif;color:#555555;margin-top:5px;" cellspacing="20"
+|style="font-family: helvetica, arial, sans-serif;font-size:2em;color:#ea8828;"|Fast Response Module
 |-
 |<div ALIGN=CENTER>
@@ Line 967: / Line 973: @@
 |-
 |<div ALIGN=CENTER>
-{| style="background:#e7e7e7;text-align:center;font-family: helvetica, arial, sans-serif;color:#555555;margin- top:5px;" cellspacing="5";
+{| style="width:204px;background:#e7e7e7;text-align:center;font-family: helvetica, arial, sans-serif;color:#555555;margin- top:5px;" cellspacing="5";
   |<html>
   <a href="https://static.igem.org/mediawiki/2010/4/41/IC_Module3.JPG"><img src="https://static.igem.org/mediawiki/2010/4/41/IC_Module3.JPG" width="200"/></a>
@@ Line 976: / Line 982: @@
 |}
 |<div ALIGN=CENTER>
-{| style="background:#e7e7e7;text-align:center;font-family: helvetica, arial, sans-serif;color:#555555;margin- top:5px;" cellspacing="5";
+{| style="width:204px;background:#e7e7e7;text-align:center;font-family: helvetica, arial, sans-serif;color:#555555;margin- top:5px;" cellspacing="5";
   |<html>
   <a href="https://static.igem.org/mediawiki/2010/1/1f/ICxyle_testing.JPG"><img src="https://static.igem.org/mediawiki/2010/1/1f/ICxyle_testing.JPG" width="200"/></a>
@@ Line 984: / Line 990: @@
 </div>
 |}
-|-
+|}
-|<html><h2>Human Practices</h2></html>
+{| style="width:900px;background:#f5f5f5;text-align:justify;font-family: helvetica, arial, sans-serif;color:#555555;margin-top:5px;" cellspacing="20"
+|style="font-family: helvetica, arial, sans-serif;font-size:2em;color:#ea8828;"|Human Practices
 |-
 |<div ALIGN=CENTER>
@@ Line 997: / Line 1,004: @@
 |}
 |<div ALIGN=CENTER>
-  {| style="background:#e7e7e7;text-align:center;font-family: helvetica, arial, sans-serif;color:#555555;margin- top:5px;" cellspacing="5";
+  {| style="width:204px;background:#e7e7e7;text-align:center;font-family: helvetica, arial, sans-serif;color:#555555;margin- top:5px;" cellspacing="5";
   |<html>
   <a href="https://static.igem.org/mediawiki/2010/1/1f/HPpanel1.jpg"><img src="https://static.igem.org/mediawiki/2010/1/1f/HPpanel1.jpg" width="200"/></a>
@@ Line 1,034: / Line 1,041: @@
 |}
 |<div ALIGN=CENTER>
-  {| style="background:#e7e7e7;text-align:center;font-family: helvetica, arial, sans-serif;color:#555555;margin- top:5px;" cellspacing="5";
+  {| style="width:204px;background:#e7e7e7;text-align:center;font-family: helvetica, arial, sans-serif;color:#555555;margin- top:5px;" cellspacing="5";
   |<html>
   <a href="https://static.igem.org/mediawiki/2010/9/9f/IC_Economist1.JPG"><img src="https://static.igem.org/mediawiki/2010/9/9f/IC_Economist1.JPG" width="200"/></a>
@@ Line 1,044: / Line 1,051: @@
 |-
 |<div ALIGN=CENTER>
-  {| style="background:#e7e7e7;text-align:center;font-family: helvetica, arial, sans-serif;color:#555555;margin- top:5px;" cellspacing="5";
+  {| style="width:204px;background:#e7e7e7;text-align:center;font-family: helvetica, arial, sans-serif;color:#555555;margin- top:5px;" cellspacing="5";
   |<html>
   <a href="https://static.igem.org/mediawiki/2010/4/4a/IC_Economist2.jpg"><img src="https://static.igem.org/mediawiki/2010/4/4a/IC_Economist2.jpg" width="200"/></a>
@@ Line 1,053: / Line 1,060: @@
 |}
 |<div ALIGN=CENTER>
-  {| style="background:#e7e7e7;text-align:center;font-family: helvetica, arial, sans-serif;color:#555555;margin- top:5px;" cellspacing="5";
+  {| style="width:204px;background:#e7e7e7;text-align:center;font-family: helvetica, arial, sans-serif;color:#555555;margin- top:5px;" cellspacing="5";
   |<html>
   <a href="https://static.igem.org/mediawiki/2010/4/40/IC_prototype1.jpg"><img src="https://static.igem.org/mediawiki/2010/4/40/IC_prototype1.jpg" width="200"/></a>
@@ Line 1,062: / Line 1,069: @@
 |}
 |<div ALIGN=CENTER>
-  {| style="background:#e7e7e7;text-align:center;font-family: helvetica, arial, sans-serif;color:#555555;margin- top:5px;" cellspacing="5";
+  {| style="width:204px;background:#e7e7e7;text-align:center;font-family: helvetica, arial, sans-serif;color:#555555;margin- top:5px;" cellspacing="5";
   |<html>
   <a href="https://static.igem.org/mediawiki/2010/3/34/IC_prototype2.jpg"><img src="https://static.igem.org/mediawiki/2010/3/34/IC_prototype2.jpg" width="200"/></a>
@@ Line 1,071: / Line 1,078: @@
 |}
 |-
-|<html><h2>School Workshops</h2></html>
+|<div ALIGN=CENTER>
+ {| style="background:#e7e7e7;text-align:center;font-family: helvetica, arial, sans-serif;color:#555555;margin- top:5px;" cellspacing="5";
+ |<html>
+ <a href="https://static.igem.org/mediawiki/2010/a/a2/IC_Concept_1.jpg"><img src="https://static.igem.org/mediawiki/2010/a/a2/IC_Concept_1.jpg" width="200"/></a>
+ </html>
+ |-
+ |This is the first design...
+</div>
+|}
+|<div ALIGN=CENTER>
+ {| style="width:204px;background:#e7e7e7;text-align:center;font-family: helvetica, arial, sans-serif;color:#555555;margin- top:5px;" cellspacing="5";
+ |<html>
+ <a href="https://static.igem.org/mediawiki/2010/4/43/IC_Prototype.jpg"><img src="https://static.igem.org/mediawiki/2010/4/43/IC_Prototype.jpg" width="200"/></a>
+ </html>
+ |-
+ |A prototype of our dipstick concept!! So cool!!!
+</div>
+|}
+|<div ALIGN=CENTER>
+ {| style="width:204px;background:#e7e7e7;text-align:center;font-family: helvetica, arial, sans-serif;color:#555555;margin- top:5px;" cellspacing="5";
+ |<html>
+ <a href="https://static.igem.org/mediawiki/2010/0/0d/IC_Concept2.jpg"><img src="https://static.igem.org/mediawiki/2010/0/0d/IC_Concept2.jpg" width="200"/></a>
+ </html>
+ |-
+ |More pictures of our awesome prototype!!!
+</div>
+|}
+|}
+{| style="width:900px;background:#f5f5f5;text-align:justify;font-family: helvetica, arial, sans-serif;color:#555555;margin-top:5px;" cellspacing="20"
+|style="font-family: helvetica, arial, sans-serif;font-size:2em;color:#ea8828;"|School Workshops
 |-
 |<div ALIGN=CENTER>
@@ Line 1,083: / Line 1,119: @@
 |}
 |<div ALIGN=CENTER>
-  {| style="background:#e7e7e7;text-align:center;font-family: helvetica, arial, sans-serif;color:#555555;margin- top:5px;" cellspacing="5";
+  {| style="width:204px;background:#e7e7e7;text-align:center;font-family: helvetica, arial, sans-serif;color:#555555;margin- top:5px;" cellspacing="5";
   |<html>
   <a href="https://static.igem.org/mediawiki/2010/f/f1/ICschool10.jpg"><img src="https://static.igem.org/mediawiki/2010/f/f1/ICschool10.jpg" width="200"/></a>
@@ Line 1,111: / Line 1,147: @@
 |}
 |<div ALIGN=CENTER>
-  {| style="background:#e7e7e7;text-align:center;font-family: helvetica, arial, sans-serif;color:#555555;margin- top:5px;" cellspacing="5";
+  {| style="width:204px;background:#e7e7e7;text-align:center;font-family: helvetica, arial, sans-serif;color:#555555;margin- top:5px;" cellspacing="5";
   |<html>
   <a href="https://static.igem.org/mediawiki/2010/e/ed/ICschool7.jpg"><img src="https://static.igem.org/mediawiki/2010/e/ed/ICschool7.jpg" width="200"/></a>
@@ Line 1,120: / Line 1,156: @@
 |}
 |<div ALIGN=CENTER>
-  {| style="background:#e7e7e7;text-align:center;font-family: helvetica, arial, sans-serif;color:#555555;margin- top:5px;" cellspacing="5";
+  {| style="width:204px;background:#e7e7e7;text-align:center;font-family: helvetica, arial, sans-serif;color:#555555;margin- top:5px;" cellspacing="5";
   |<html>
   <a href="https://static.igem.org/mediawiki/2010/a/a0/ICschool13.jpg"><img src="https://static.igem.org/mediawiki/2010/a/a0/ICschool13.jpg" width="200"/></a>
@@ Line 1,130: / Line 1,166: @@
 |-
 |<div ALIGN=CENTER>
-  {| style="background:#e7e7e7;text-align:center;font-family: helvetica, arial, sans-serif;color:#555555;margin- top:5px;" cellspacing="5";
+  {| style="width:204px;background:#e7e7e7;text-align:center;font-family: helvetica, arial, sans-serif;color:#555555;margin- top:5px;" cellspacing="5";
   |<html>
   <a href="https://static.igem.org/mediawiki/2010/a/a5/ICschool9.jpg"><img src="https://static.igem.org/mediawiki/2010/a/a5/ICschool9.jpg" width="200"/></a>
@@ Line 1,158: / Line 1,194: @@
 |-
 |<div ALIGN=CENTER>
-  {| style="background:#e7e7e7;text-align:center;font-family: helvetica, arial, sans-serif;color:#555555;margin- top:5px;" cellspacing="5";
+  {| style="width:204px;background:#e7e7e7;text-align:center;font-family: helvetica, arial, sans-serif;color:#555555;margin- top:5px;" cellspacing="5";
   |<html>
   <a href="https://static.igem.org/mediawiki/2010/a/a5/ICschool2.jpg"><img src="https://static.igem.org/mediawiki/2010/a/a5/ICschool2.jpg" width="200"/></a>