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Team:INSA-Lyon/Project/Stage3/Theory/curli promoter
From 2010.igem.org
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| - | <p> The Curli promoter is from the chassi E.Coli PHL1273. It is a kanamycin resistant chassi containing a plasmid with the full-length intergenic region between csgD and csgB genes (approximately 450 bp) including csgD and csgBA promoters. A gfp reporter gene is placed downstream the csgBA promoter (curli promoter), in order to quantify its activity. This chassis is adherent but all the genes involving in adherence structure synthesis (csgA,B,E,F and G genes) are in the chromosomal DNA, not in the plasmid DNA.</p><br> | + | <p> The Curli promoter is from the chassi E.Coli PHL1273. It is a kanamycin resistant chassi containing a plasmid with the full-length intergenic region between csgD and csgB genes (approximately 450 bp) including csgD and csgBA promoters. A gfp reporter gene is placed downstream the csgBA promoter (curli promoter), in order to quantify its activity. The promoter csgD is under the control of the protein <a href="https://2010.igem.org/Team:INSA-Lyon/Project/Stage3/Theory/ompR">ompR</a>. This chassis is adherent but all the genes involving in adherence structure synthesis (csgA,B,E,F and G genes) are in the chromosomal DNA, not in the plasmid DNA.</p><br> |
| - | <p></p><br> | + | <p>The curli production involves two divergent operons:</p><br> |
<p></p><br> | <p></p><br> | ||
Revision as of 14:01, 26 October 2010
Curli Promoter
The Curli promoter is from the chassi E.Coli PHL1273. It is a kanamycin resistant chassi containing a plasmid with the full-length intergenic region between csgD and csgB genes (approximately 450 bp) including csgD and csgBA promoters. A gfp reporter gene is placed downstream the csgBA promoter (curli promoter), in order to quantify its activity. The promoter csgD is under the control of the protein ompR. This chassis is adherent but all the genes involving in adherence structure synthesis (csgA,B,E,F and G genes) are in the chromosomal DNA, not in the plasmid DNA.
The curli production involves two divergent operons:
