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Team:INSA-Lyon/Project/Stage1/Results
From 2010.igem.org
Results
The pILI1 plasmid
The transformed bacteria had been spread on LB agar plate, with or without 7% of glucose and RedNile dye. As you can see on the photos below, the bacteria appear pink colored directly on plates supplemented with glucose. However, the control without glucose doesn't reveal a pink coloration.
Plates with pILI1 LB with or without Glucose 7% and RedNile dye.
With a liquid culture on LB complemented with glucose (7%) and RedNile dye, we observed the fluorescence by microscopy.
Observation of fluorescent granule by microscopy
The separated genes
We extracted the gene phaC from the plasmid of synthesis of Mr Gene. The gene had been successfully transferred in the pSBC3 plasmid. We send the product to the registry: BBa_K342001
