Team:INSA-Lyon/Project/Future direction

From 2010.igem.org

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<br><p style="text-indent: 30px; text-align:justify;">Our project was full of ideas and the time too short to achieve until the end our projects. So there is still plenty to do get and improve results.<br>
<br><p style="text-indent: 30px; text-align:justify;">Our project was full of ideas and the time too short to achieve until the end our projects. So there is still plenty to do get and improve results.<br>
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<h4><font color="purple">Production</font></h4>
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<h3><font color="purple">Production</font></h3>
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We managed to produce granules and a <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K342001">new operational PhaC part </a> but we didn't send the complete operon (phaCAB) which would able any team to produce PHB granules inside E.coli.<br></p>
We managed to produce granules and a <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K342001">new operational PhaC part </a> but we didn't send the complete operon (phaCAB) which would able any team to produce PHB granules inside E.coli.<br></p>
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<h4><font color="purple">Uses</font></h4>
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<h3><font color="purple">Uses</font></h3>
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<p>We added a very interesting part <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K342002">(phasin-phasin-intein)</a> to the registry which should bind to the PHB granule surface and enable an easier purification way for molecules of interest thanks to the self cleaving protein. We first wanted to test this part with a fusion GFP but interesting research to adapt this purification way for proteins or molecule of interest can be carried out further.<br>
<p>We added a very interesting part <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K342002">(phasin-phasin-intein)</a> to the registry which should bind to the PHB granule surface and enable an easier purification way for molecules of interest thanks to the self cleaving protein. We first wanted to test this part with a fusion GFP but interesting research to adapt this purification way for proteins or molecule of interest can be carried out further.<br>
Concerning the use of the granule as lipid storage, we didn't manage to co-localize the granules and the lycopene inside the bacteria. We couldn't manage to differentiate the lycopene and PHB by fluorescence microscopy. A granules extraction experiment could be perform after having induce lycopene production and then look for lycopene inside those extractions. So this idea has still to be deepen down.
Concerning the use of the granule as lipid storage, we didn't manage to co-localize the granules and the lycopene inside the bacteria. We couldn't manage to differentiate the lycopene and PHB by fluorescence microscopy. A granules extraction experiment could be perform after having induce lycopene production and then look for lycopene inside those extractions. So this idea has still to be deepen down.
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<h4><font color="purple">Regulation</font></h4>
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<h3><font color="purple">Regulation</font></h3><br />
<p>We characterize the natural curli promoter inside a plasmid and we wanted to link those results with the ones of our <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K342000">designed curli promoter</a>. But we were not able to realize the measurements to conclude about the performance of our biobrick.</p>
<p>We characterize the natural curli promoter inside a plasmid and we wanted to link those results with the ones of our <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K342000">designed curli promoter</a>. But we were not able to realize the measurements to conclude about the performance of our biobrick.</p>
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Revision as of 22:26, 27 October 2010





Future Direction


Our project was full of ideas and the time too short to achieve until the end our projects. So there is still plenty to do get and improve results.


Production


We managed to produce granules and a new operational PhaC part but we didn't send the complete operon (phaCAB) which would able any team to produce PHB granules inside E.coli.




Uses


We added a very interesting part (phasin-phasin-intein) to the registry which should bind to the PHB granule surface and enable an easier purification way for molecules of interest thanks to the self cleaving protein. We first wanted to test this part with a fusion GFP but interesting research to adapt this purification way for proteins or molecule of interest can be carried out further.
Concerning the use of the granule as lipid storage, we didn't manage to co-localize the granules and the lycopene inside the bacteria. We couldn't manage to differentiate the lycopene and PHB by fluorescence microscopy. A granules extraction experiment could be perform after having induce lycopene production and then look for lycopene inside those extractions. So this idea has still to be deepen down.




Regulation


We characterize the natural curli promoter inside a plasmid and we wanted to link those results with the ones of our designed curli promoter. But we were not able to realize the measurements to conclude about the performance of our biobrick.