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| | <!-- | | <!-- |
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| - | mso-border-bottom-themecolor:accent1;padding:0cm 0cm 4.0pt 0cm'> | + | mso-border-bottom-themecolor:accent1;padding:0cm 1cm 4.0pt 0cm'> |
| | | | |
| - | <p class=MsoTitle align=center style='text-align:center'><span lang=EN-US>IIT | + | <p align=center><embed src="https://static.igem.org/mediawiki/igem.org/5/51/Nish1.swf" width=900 height=500></p> |
| - | Delhi iGEM Wiki</span></p>
| + | |
| - | | + | |
| - | </div>
| + | |
| - | | + | |
| - | <p class=MsoNormal><span lang=EN-US><o:p> </o:p></span></p>
| + | |
| - | | + | |
| - | <h2><u><span lang=EN-US>Renin Angiotensin Aldosterone System (RAAS)<o:p></o:p></span></u></h2>
| + | |
| - | | + | |
| - | <p class=MsoNormal><span lang=EN-US><o:p> </o:p></span></p>
| + | |
| - | | + | |
| - | <h3><span lang=EN-US>Introduction</span></h3>
| + | |
| - | | + | |
| - | <p class=MsoNormal style='text-align:justify'><span lang=EN-US>The Renin
| + | |
| - | Angiotensin Aldosterone System is a hormone system in the human body that is
| + | |
| - | responsible for the regulation of blood pressure and fluid balance.</span></p>
| + | |
| - | | + | |
| - | <h3 style='text-align:justify'><span lang=EN-US>Functioning</span></h3>
| + | |
| - | | + | |
| - | <p class=MsoNormal style='text-align:justify'><span lang=EN-US>A decrease in
| + | |
| - | renal perfusion causes the release of the enzyme renin which subsequently
| + | |
| - | cleaves angiotensinogen to angiotensin I. This is further converted to
| + | |
| - | angiotensin II by angiotensin-converting enzyme (ACE). The bioactive product,
| + | |
| - | angiotensin II, causes the constriction of blood vessels, leading to an
| + | |
| - | increased blood pressure. It is also responsible for the secretion of
| + | |
| - | aldosterone by the adrenal cortex, which acts by increasing the re-absorption
| + | |
| - | of sodium and water into the blood. Hence, the fluid volume as well as the
| + | |
| - | blood pressure is maintained in the body.</span></p>
| + | |
| - | | + | |
| - | <h2><u><span lang=EN-US>The α-hemolysin System in <i style='mso-bidi-font-style:
| + | |
| - | normal'>E.coli<o:p></o:p></i></span></u></h2>
| + | |
| - | | + | |
| - | <p class=MsoNormal><span lang=EN-US><o:p> </o:p></span></p>
| + | |
| - | | + | |
| - | <h3><span lang=EN-US>Introduction</span></h3>
| + | |
| - | | + | |
| - | <p class=MsoNormal style='text-align:justify'><span lang=EN-US>The
| + | |
| - | alpha-hemolysin system is a type I secretion system found in wild type <i
| + | |
| - | style='mso-bidi-font-style:normal'>E.coli </i>which enable them to cause
| + | |
| - | infection in the urinary tract and is an important virulence factor because of
| + | |
| - | its cytotoxic and cytolytic activity against a variety of mammalian cells. </span></p>
| + | |
| - | | + | |
| - | <h3 style='text-align:justify'><span lang=EN-US>Functioning</span></h3>
| + | |
| - | | + | |
| - | <p class=MsoNormal style='text-align:justify'><span lang=EN-US>The hemolysin
| + | |
| - | system originates from a series of hly genes. These genes are present in the
| + | |
| - | series <i style='mso-bidi-font-style:normal'>hlyC – hlyA – hlyB – hlyD</i>. Of
| + | |
| - | these, <i style='mso-bidi-font-style:normal'>hlyA</i> codes for the protein
| + | |
| - | that is responsible for the infection. Hly B and D are membrane proteins that
| + | |
| - | work in coordination for the secretion of HlyA. The synthesis, activation and
| + | |
| - | secretion of HlyA in <i style='mso-bidi-font-style:normal'>E.coli </i>is
| + | |
| - | determined by the <i style='mso-bidi-font-style:normal'>hlyCABD</i> operon. This
| + | |
| - | operon is either located either on the chromosome or on transmissible plasmids.
| + | |
| - | | + | |
| - | </span></p>
| + | |
| - | | + | |
| - | <p class=MsoNormal style='text-align:justify'><span lang=EN-US>It has been
| + | |
| - | shown that this system can be used to produce and secrete a protein of our
| + | |
| - | choice if the protein contains 60 amino acid residues from the carboxy terminal
| + | |
| - | end of the HlyA protein. </span></p>
| + | |
| - | | + | |
| - | <h2><u><span lang=EN-US>Our iGEM 2010 Project<o:p></o:p></span></u></h2>
| + | |
| - | | + | |
| - | <p class=MsoNormal><span lang=EN-US><o:p> </o:p></span></p>
| + | |
| - | | + | |
| - | <h3><span lang=EN-US>Introduction</span></h3>
| + | |
| - | | + | |
| - | <p class=MsoNormal style='text-align:justify'><span lang=EN-US>Our aim is to
| + | |
| - | create an online elicitor detection and response in a flow system, which can
| + | |
| - | function in the detection of high blood pressure and help reduce it by
| + | |
| - | releasing renin inhibitors into the circulatory system. This kind of a system
| + | |
| - | would be helpful to patients with a hypertensive condition. </span></p>
| + | |
| - | | + | |
| - | <h3><span lang=EN-US>Approach</span></h3>
| + | |
| - | | + | |
| - | <p class=MsoNormal><span lang=EN-US>Since the blood pressure system is not
| + | |
| - | really a chemical signal, what we have assumed is that the blood pressure can
| + | |
| - | be easily converted into a chemical signal. Having assumed this, we will use a
| + | |
| - | chemical signal such as IPTG or AHL as the elicitor to produce appropriate
| + | |
| - | amounts of renin inhibitor. For easy detection and verification, we are
| + | |
| - | planning to use GFP in place of renin inhibitor which can easily be replaced
| + | |
| - | later.</span></p>
| + | |
| - | | + | |
| - | <p class=MsoNormal><span lang=EN-US>For this we will be introducing two vectors
| + | |
| - | into <i style='mso-bidi-font-style:normal'>E. coli </i>as shown below.</span></p>
| + | |
| - | | + | |
| - | <p class=MsoNormal><span style='mso-ansi-language:EN-IN;mso-fareast-language:
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| + | |
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| + | |
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| + | |
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| + | |
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| + | |
| - | src="IIT+Delhi+iGEM+Wiki_files/image002.gif" alt=Vector1.png v:shapes="Picture_x0020_0"><![endif]></span><span
| + | |
| - | lang=EN-US><span style='mso-spacerun:yes'> </span><span
| + | |
| - | style='mso-tab-count:1'> </span></span><span style='mso-ansi-language:
| + | |
| - | EN-IN;mso-fareast-language:EN-IN;mso-bidi-language:HI;mso-no-proof:yes'><!--[if gte vml 1]><v:shape
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| + | |
| - | </v:shape><![endif]--><![if !vml]><img width=260 height=283
| + | |
| - | src="IIT+Delhi+iGEM+Wiki_files/image004.gif" alt=Vector2.png v:shapes="Picture_x0020_1"><![endif]></span></p>
| + | |
| - | | + | |
| - | <p class=MsoNormal><span lang=EN-US><o:p> </o:p></span></p>
| + | |
| - | | + | |
| - | <p class=MsoNormal style='text-align:justify'><span lang=EN-US>The first vector
| + | |
| - | which contains the GFP-hlyA chimeric sequence with pLac promoter is used to
| + | |
| - | produce the chimera GFP protein which contains 62 amino acid residues from the
| + | |
| - | C terminal of hlyA protein, a prerequisite for the secretion of the protein
| + | |
| - | outside the cell body. Forming a chimera will not in any way change the
| + | |
| - | structure or function as per literature.</span></p>
| + | |
| - | | + | |
| - | <p class=MsoNormal><span lang=EN-US><o:p> </o:p></span></p>
| + | |
| - | | + | |
| - | <p class=MsoNormal><span lang=EN-US>The other vector contains the hlyB and hlyD
| + | |
| - | sequences with ptet promoter sequence. These two proteins will be
| + | |
| - | constitutively expressed. These proteins are present in the membrane and
| + | |
| - | provide for an outlet of hlyA or chimeras with 62 amino acid residues of the C
| + | |
| - | terminal of hlyA.</span></p>
| + | |
| - | | + | |
| - | <p class=MsoNormal><span lang=EN-US>After we are successful in obtaining
| + | |
| - | GFP-hlyA chimera outside the cell body, our next task will be to immobilize
| + | |
| - | these cells in a flow system and characterize the flow-expression profile. We
| + | |
| - | would want to keep the cells in G0 phase so as to have continuous expression of
| + | |
| - | the protein without cell death</span></p>
| + | |
| - | | + | |
| - | <p class=MsoNormal><span lang=EN-US>We will also be working towards minimizing
| + | |
| - | the toxins and other products so that such a system can finally be thought fit
| + | |
| - | for incorporating and integrating with the circulatory system in our body.</span></p>
| + | |
| - | | + | |
| - | <p class=MsoNormal><span lang=EN-US><o:p> </o:p></span></p>
| + | |
| - | | + | |
| - | <p class=MsoNormal style='text-align:justify'><span lang=EN-US><o:p> </o:p></span></p>
| + | |
| | | | |
| | + | <p><h2>Special comments:</h2> |
| | + | 1.Plasmids acquired from Karolinska Institutet, Solna, Sweden. <br> |
| | + | 2.Safety page in 'Work' link. |
| | </div> | | </div> |
| | | | |
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| | </html> | | </html> |
| - |
| |
| - |
| |
| - | {| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
| |
| - | !align="center"|[[Team:IIT_Delhi_1|Home]]
| |
| - | !align="center"|[[Team:IIT_Delhi_1/Team|Team]]
| |
| - | !align="center"|[https://igem.org/Team.cgi?year=2010&team_name=IIT_Delhi_1 Official Team Profile]
| |
| - | !align="center"|[[Team:IIT_Delhi_1/Project|Project]]
| |
| - | !align="center"|[[Team:IIT_Delhi_1/Parts|Parts Submitted to the Registry]]
| |
| - | !align="center"|[[Team:IIT_Delhi_1/Modeling|Modeling]]
| |
| - | !align="center"|[[Team:IIT_Delhi_1/Notebook|Notebook]]
| |
| - | !align="center"|[[Team:IIT_Delhi_1/Safety|Safety]]
| |
| - | |}
| |
"
