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| - | <html>
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| - |
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| - |
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| - | <head>
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| - |
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| - | <style>
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| - | <!--
| |
| - | /* Font Definitions */
| |
| - | @font-face
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| - | {font-family:Wingdings;
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| - | panose-1:5 0 0 0 0 0 0 0 0 0;
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| - | mso-font-charset:2;
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| - | mso-generic-font-family:auto;
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| - | mso-font-pitch:variable;
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| - | mso-font-signature:0 268435456 0 0 -2147483648 0;}
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| - | @font-face
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| - | {font-family:Mangal;
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| - | panose-1:2 4 5 3 5 2 3 3 2 2;
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| - | mso-font-charset:0;
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| - | mso-generic-font-family:roman;
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| - | mso-font-pitch:variable;
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| - | mso-font-signature:32771 0 0 0 1 0;}
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| - | @font-face
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| - | {font-family:"Cambria Math";
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| - | panose-1:2 4 5 3 5 4 6 3 2 4;
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| - | mso-font-charset:1;
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| - | mso-generic-font-family:roman;
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| - | mso-font-format:other;
| |
| - | mso-font-pitch:variable;
| |
| - | mso-font-signature:0 0 0 0 0 0;}
| |
| - | @font-face
| |
| - | {font-family:Cambria;
| |
| - | panose-1:2 4 5 3 5 4 6 3 2 4;
| |
| - | mso-font-charset:0;
| |
| - | mso-generic-font-family:roman;
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| - | mso-font-pitch:variable;
| |
| - | mso-font-signature:-1610611985 1073741899 0 0 159 0;}
| |
| - | @font-face
| |
| - | {font-family:Calibri;
| |
| - | panose-1:2 15 5 2 2 2 4 3 2 4;
| |
| - | mso-font-charset:0;
| |
| - | mso-generic-font-family:swiss;
| |
| - | mso-font-pitch:variable;
| |
| - | mso-font-signature:-1610611985 1073750139 0 0 159 0;}
| |
| - | @font-face
| |
| - | {font-family:Tahoma;
| |
| - | panose-1:2 11 6 4 3 5 4 4 2 4;
| |
| - | mso-font-charset:0;
| |
| - | mso-generic-font-family:swiss;
| |
| - | mso-font-format:other;
| |
| - | mso-font-pitch:variable;
| |
| - | mso-font-signature:3 0 0 0 1 0;}
| |
| - | /* Style Definitions */
| |
| - | p.MsoNormal, li.MsoNormal, div.MsoNormal
| |
| - | {mso-style-unhide:no;
| |
| - | mso-style-qformat:yes;
| |
| - | mso-style-parent:"";
| |
| - | margin-top:0cm;
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| - | margin-right:0cm;
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| - | margin-bottom:10.0pt;
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| - | margin-left:0cm;
| |
| - | line-height:115%;
| |
| - | mso-pagination:widow-orphan;
| |
| - | font-size:11.0pt;
| |
| - | font-family:"Calibri","sans-serif";
| |
| - | mso-ascii-font-family:Calibri;
| |
| - | mso-ascii-theme-font:minor-latin;
| |
| - | mso-fareast-font-family:Calibri;
| |
| - | mso-fareast-theme-font:minor-latin;
| |
| - | mso-hansi-font-family:Calibri;
| |
| - | mso-hansi-theme-font:minor-latin;
| |
| - | mso-bidi-font-family:Mangal;
| |
| - | mso-bidi-theme-font:minor-bidi;
| |
| - | mso-ansi-language:EN-US;
| |
| - | mso-fareast-language:EN-US;
| |
| - | mso-bidi-language:AR-SA;}
| |
| - | h2
| |
| - | {mso-style-priority:9;
| |
| - | mso-style-qformat:yes;
| |
| - | mso-style-link:"Heading 2 Char";
| |
| - | mso-style-next:Normal;
| |
| - | margin-top:10.0pt;
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| - | margin-right:0cm;
| |
| - | margin-bottom:0cm;
| |
| - | margin-left:0cm;
| |
| - | margin-bottom:.0001pt;
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| - | line-height:115%;
| |
| - | mso-pagination:widow-orphan lines-together;
| |
| - | page-break-after:avoid;
| |
| - | mso-outline-level:2;
| |
| - | font-size:13.0pt;
| |
| - | font-family:"Cambria","serif";
| |
| - | mso-ascii-font-family:Cambria;
| |
| - | mso-ascii-theme-font:major-latin;
| |
| - | mso-fareast-font-family:"Times New Roman";
| |
| - | mso-fareast-theme-font:major-fareast;
| |
| - | mso-hansi-font-family:Cambria;
| |
| - | mso-hansi-theme-font:major-latin;
| |
| - | mso-bidi-font-family:Mangal;
| |
| - | mso-bidi-theme-font:major-bidi;
| |
| - | color:#4F81BD;
| |
| - | mso-themecolor:accent1;
| |
| - | mso-ansi-language:EN-US;
| |
| - | mso-fareast-language:EN-US;
| |
| - | mso-bidi-language:AR-SA;}
| |
| - | h3
| |
| - | {mso-style-priority:9;
| |
| - | mso-style-qformat:yes;
| |
| - | mso-style-link:"Heading 3 Char";
| |
| - | mso-style-next:Normal;
| |
| - | margin-top:10.0pt;
| |
| - | margin-right:0cm;
| |
| - | margin-bottom:0cm;
| |
| - | margin-left:0cm;
| |
| - | margin-bottom:.0001pt;
| |
| - | line-height:115%;
| |
| - | mso-pagination:widow-orphan lines-together;
| |
| - | page-break-after:avoid;
| |
| - | mso-outline-level:3;
| |
| - | font-size:11.0pt;
| |
| - | font-family:"Cambria","serif";
| |
| - | mso-ascii-font-family:Cambria;
| |
| - | mso-ascii-theme-font:major-latin;
| |
| - | mso-fareast-font-family:"Times New Roman";
| |
| - | mso-fareast-theme-font:major-fareast;
| |
| - | mso-hansi-font-family:Cambria;
| |
| - | mso-hansi-theme-font:major-latin;
| |
| - | mso-bidi-font-family:Mangal;
| |
| - | mso-bidi-theme-font:major-bidi;
| |
| - | color:#4F81BD;
| |
| - | mso-themecolor:accent1;
| |
| - | mso-ansi-language:EN-US;
| |
| - | mso-fareast-language:EN-US;
| |
| - | mso-bidi-language:AR-SA;}
| |
| - | p.MsoTitle, li.MsoTitle, div.MsoTitle
| |
| - | {mso-style-priority:10;
| |
| - | mso-style-unhide:no;
| |
| - | mso-style-qformat:yes;
| |
| - | mso-style-link:"Title Char";
| |
| - | mso-style-next:Normal;
| |
| - | margin-top:0cm;
| |
| - | margin-right:0cm;
| |
| - | margin-bottom:15.0pt;
| |
| - | margin-left:0cm;
| |
| - | mso-add-space:auto;
| |
| - | mso-pagination:widow-orphan;
| |
| - | border:none;
| |
| - | mso-border-bottom-alt:solid #4F81BD 1.0pt;
| |
| - | mso-border-bottom-themecolor:accent1;
| |
| - | padding:0cm;
| |
| - | mso-padding-alt:0cm 0cm 4.0pt 0cm;
| |
| - | font-size:26.0pt;
| |
| - | font-family:"Cambria","serif";
| |
| - | mso-ascii-font-family:Cambria;
| |
| - | mso-ascii-theme-font:major-latin;
| |
| - | mso-fareast-font-family:"Times New Roman";
| |
| - | mso-fareast-theme-font:major-fareast;
| |
| - | mso-hansi-font-family:Cambria;
| |
| - | mso-hansi-theme-font:major-latin;
| |
| - | mso-bidi-font-family:Mangal;
| |
| - | mso-bidi-theme-font:major-bidi;
| |
| - | color:#17365D;
| |
| - | mso-themecolor:text2;
| |
| - | mso-themeshade:191;
| |
| - | letter-spacing:.25pt;
| |
| - | mso-font-kerning:14.0pt;
| |
| - | mso-ansi-language:EN-US;
| |
| - | mso-fareast-language:EN-US;
| |
| - | mso-bidi-language:AR-SA;}
| |
| - | p.MsoTitleCxSpFirst, li.MsoTitleCxSpFirst, div.MsoTitleCxSpFirst
| |
| - | {mso-style-priority:10;
| |
| - | mso-style-unhide:no;
| |
| - | mso-style-qformat:yes;
| |
| - | mso-style-link:"Title Char";
| |
| - | mso-style-next:Normal;
| |
| - | mso-style-type:export-only;
| |
| - | margin:0cm;
| |
| - | margin-bottom:.0001pt;
| |
| - | mso-add-space:auto;
| |
| - | mso-pagination:widow-orphan;
| |
| - | border:none;
| |
| - | mso-border-bottom-alt:solid #4F81BD 1.0pt;
| |
| - | mso-border-bottom-themecolor:accent1;
| |
| - | padding:0cm;
| |
| - | mso-padding-alt:0cm 0cm 4.0pt 0cm;
| |
| - | font-size:26.0pt;
| |
| - | font-family:"Cambria","serif";
| |
| - | mso-ascii-font-family:Cambria;
| |
| - | mso-ascii-theme-font:major-latin;
| |
| - | mso-fareast-font-family:"Times New Roman";
| |
| - | mso-fareast-theme-font:major-fareast;
| |
| - | mso-hansi-font-family:Cambria;
| |
| - | mso-hansi-theme-font:major-latin;
| |
| - | mso-bidi-font-family:Mangal;
| |
| - | mso-bidi-theme-font:major-bidi;
| |
| - | color:#17365D;
| |
| - | mso-themecolor:text2;
| |
| - | mso-themeshade:191;
| |
| - | letter-spacing:.25pt;
| |
| - | mso-font-kerning:14.0pt;
| |
| - | mso-ansi-language:EN-US;
| |
| - | mso-fareast-language:EN-US;
| |
| - | mso-bidi-language:AR-SA;}
| |
| - | p.MsoTitleCxSpMiddle, li.MsoTitleCxSpMiddle, div.MsoTitleCxSpMiddle
| |
| - | {mso-style-priority:10;
| |
| - | mso-style-unhide:no;
| |
| - | mso-style-qformat:yes;
| |
| - | mso-style-link:"Title Char";
| |
| - | mso-style-next:Normal;
| |
| - | mso-style-type:export-only;
| |
| - | margin:0cm;
| |
| - | margin-bottom:.0001pt;
| |
| - | mso-add-space:auto;
| |
| - | mso-pagination:widow-orphan;
| |
| - | border:none;
| |
| - | mso-border-bottom-alt:solid #4F81BD 1.0pt;
| |
| - | mso-border-bottom-themecolor:accent1;
| |
| - | padding:0cm;
| |
| - | mso-padding-alt:0cm 0cm 4.0pt 0cm;
| |
| - | font-size:26.0pt;
| |
| - | font-family:"Cambria","serif";
| |
| - | mso-ascii-font-family:Cambria;
| |
| - | mso-ascii-theme-font:major-latin;
| |
| - | mso-fareast-font-family:"Times New Roman";
| |
| - | mso-fareast-theme-font:major-fareast;
| |
| - | mso-hansi-font-family:Cambria;
| |
| - | mso-hansi-theme-font:major-latin;
| |
| - | mso-bidi-font-family:Mangal;
| |
| - | mso-bidi-theme-font:major-bidi;
| |
| - | color:#17365D;
| |
| - | mso-themecolor:text2;
| |
| - | mso-themeshade:191;
| |
| - | letter-spacing:.25pt;
| |
| - | mso-font-kerning:14.0pt;
| |
| - | mso-ansi-language:EN-US;
| |
| - | mso-fareast-language:EN-US;
| |
| - | mso-bidi-language:AR-SA;}
| |
| - | p.MsoTitleCxSpLast, li.MsoTitleCxSpLast, div.MsoTitleCxSpLast
| |
| - | {mso-style-priority:10;
| |
| - | mso-style-unhide:no;
| |
| - | mso-style-qformat:yes;
| |
| - | mso-style-link:"Title Char";
| |
| - | mso-style-next:Normal;
| |
| - | mso-style-type:export-only;
| |
| - | margin-top:0cm;
| |
| - | margin-right:0cm;
| |
| - | margin-bottom:15.0pt;
| |
| - | margin-left:0cm;
| |
| - | mso-add-space:auto;
| |
| - | mso-pagination:widow-orphan;
| |
| - | border:none;
| |
| - | mso-border-bottom-alt:solid #4F81BD 1.0pt;
| |
| - | mso-border-bottom-themecolor:accent1;
| |
| - | padding:0cm;
| |
| - | mso-padding-alt:0cm 0cm 4.0pt 0cm;
| |
| - | font-size:26.0pt;
| |
| - | font-family:"Cambria","serif";
| |
| - | mso-ascii-font-family:Cambria;
| |
| - | mso-ascii-theme-font:major-latin;
| |
| - | mso-fareast-font-family:"Times New Roman";
| |
| - | mso-fareast-theme-font:major-fareast;
| |
| - | mso-hansi-font-family:Cambria;
| |
| - | mso-hansi-theme-font:major-latin;
| |
| - | mso-bidi-font-family:Mangal;
| |
| - | mso-bidi-theme-font:major-bidi;
| |
| - | color:#17365D;
| |
| - | mso-themecolor:text2;
| |
| - | mso-themeshade:191;
| |
| - | letter-spacing:.25pt;
| |
| - | mso-font-kerning:14.0pt;
| |
| - | mso-ansi-language:EN-US;
| |
| - | mso-fareast-language:EN-US;
| |
| - | mso-bidi-language:AR-SA;}
| |
| - | p.MsoAcetate, li.MsoAcetate, div.MsoAcetate
| |
| - | {mso-style-noshow:yes;
| |
| - | mso-style-priority:99;
| |
| - | mso-style-link:"Balloon Text Char";
| |
| - | margin:0cm;
| |
| - | margin-bottom:.0001pt;
| |
| - | mso-pagination:widow-orphan;
| |
| - | font-size:8.0pt;
| |
| - | font-family:"Tahoma","sans-serif";
| |
| - | mso-fareast-font-family:Calibri;
| |
| - | mso-fareast-theme-font:minor-latin;
| |
| - | mso-bidi-font-family:Tahoma;
| |
| - | mso-ansi-language:EN-US;
| |
| - | mso-fareast-language:EN-US;
| |
| - | mso-bidi-language:AR-SA;}
| |
| - | p.MsoListParagraph, li.MsoListParagraph, div.MsoListParagraph
| |
| - | {mso-style-priority:34;
| |
| - | mso-style-unhide:no;
| |
| - | mso-style-qformat:yes;
| |
| - | margin-top:0cm;
| |
| - | margin-right:0cm;
| |
| - | margin-bottom:10.0pt;
| |
| - | margin-left:36.0pt;
| |
| - | mso-add-space:auto;
| |
| - | line-height:115%;
| |
| - | mso-pagination:widow-orphan;
| |
| - | font-size:11.0pt;
| |
| - | font-family:"Calibri","sans-serif";
| |
| - | mso-ascii-font-family:Calibri;
| |
| - | mso-ascii-theme-font:minor-latin;
| |
| - | mso-fareast-font-family:Calibri;
| |
| - | mso-fareast-theme-font:minor-latin;
| |
| - | mso-hansi-font-family:Calibri;
| |
| - | mso-hansi-theme-font:minor-latin;
| |
| - | mso-bidi-font-family:Mangal;
| |
| - | mso-bidi-theme-font:minor-bidi;
| |
| - | mso-ansi-language:EN-US;
| |
| - | mso-fareast-language:EN-US;
| |
| - | mso-bidi-language:AR-SA;}
| |
| - | p.MsoListParagraphCxSpFirst, li.MsoListParagraphCxSpFirst, div.MsoListParagraphCxSpFirst
| |
| - | {mso-style-priority:34;
| |
| - | mso-style-unhide:no;
| |
| - | mso-style-qformat:yes;
| |
| - | mso-style-type:export-only;
| |
| - | margin-top:0cm;
| |
| - | margin-right:0cm;
| |
| - | margin-bottom:0cm;
| |
| - | margin-left:36.0pt;
| |
| - | margin-bottom:.0001pt;
| |
| - | mso-add-space:auto;
| |
| - | line-height:115%;
| |
| - | mso-pagination:widow-orphan;
| |
| - | font-size:11.0pt;
| |
| - | font-family:"Calibri","sans-serif";
| |
| - | mso-ascii-font-family:Calibri;
| |
| - | mso-ascii-theme-font:minor-latin;
| |
| - | mso-fareast-font-family:Calibri;
| |
| - | mso-fareast-theme-font:minor-latin;
| |
| - | mso-hansi-font-family:Calibri;
| |
| - | mso-hansi-theme-font:minor-latin;
| |
| - | mso-bidi-font-family:Mangal;
| |
| - | mso-bidi-theme-font:minor-bidi;
| |
| - | mso-ansi-language:EN-US;
| |
| - | mso-fareast-language:EN-US;
| |
| - | mso-bidi-language:AR-SA;}
| |
| - | p.MsoListParagraphCxSpMiddle, li.MsoListParagraphCxSpMiddle, div.MsoListParagraphCxSpMiddle
| |
| - | {mso-style-priority:34;
| |
| - | mso-style-unhide:no;
| |
| - | mso-style-qformat:yes;
| |
| - | mso-style-type:export-only;
| |
| - | margin-top:0cm;
| |
| - | margin-right:0cm;
| |
| - | margin-bottom:0cm;
| |
| - | margin-left:36.0pt;
| |
| - | margin-bottom:.0001pt;
| |
| - | mso-add-space:auto;
| |
| - | line-height:115%;
| |
| - | mso-pagination:widow-orphan;
| |
| - | font-size:11.0pt;
| |
| - | font-family:"Calibri","sans-serif";
| |
| - | mso-ascii-font-family:Calibri;
| |
| - | mso-ascii-theme-font:minor-latin;
| |
| - | mso-fareast-font-family:Calibri;
| |
| - | mso-fareast-theme-font:minor-latin;
| |
| - | mso-hansi-font-family:Calibri;
| |
| - | mso-hansi-theme-font:minor-latin;
| |
| - | mso-bidi-font-family:Mangal;
| |
| - | mso-bidi-theme-font:minor-bidi;
| |
| - | mso-ansi-language:EN-US;
| |
| - | mso-fareast-language:EN-US;
| |
| - | mso-bidi-language:AR-SA;}
| |
| - | p.MsoListParagraphCxSpLast, li.MsoListParagraphCxSpLast, div.MsoListParagraphCxSpLast
| |
| - | {mso-style-priority:34;
| |
| - | mso-style-unhide:no;
| |
| - | mso-style-qformat:yes;
| |
| - | mso-style-type:export-only;
| |
| - | margin-top:0cm;
| |
| - | margin-right:0cm;
| |
| - | margin-bottom:10.0pt;
| |
| - | margin-left:36.0pt;
| |
| - | mso-add-space:auto;
| |
| - | line-height:115%;
| |
| - | mso-pagination:widow-orphan;
| |
| - | font-size:11.0pt;
| |
| - | font-family:"Calibri","sans-serif";
| |
| - | mso-ascii-font-family:Calibri;
| |
| - | mso-ascii-theme-font:minor-latin;
| |
| - | mso-fareast-font-family:Calibri;
| |
| - | mso-fareast-theme-font:minor-latin;
| |
| - | mso-hansi-font-family:Calibri;
| |
| - | mso-hansi-theme-font:minor-latin;
| |
| - | mso-bidi-font-family:Mangal;
| |
| - | mso-bidi-theme-font:minor-bidi;
| |
| - | mso-ansi-language:EN-US;
| |
| - | mso-fareast-language:EN-US;
| |
| - | mso-bidi-language:AR-SA;}
| |
| - | span.TitleChar
| |
| - | {mso-style-name:"Title Char";
| |
| - | mso-style-priority:10;
| |
| - | mso-style-unhide:no;
| |
| - | mso-style-locked:yes;
| |
| - | mso-style-link:Title;
| |
| - | mso-ansi-font-size:26.0pt;
| |
| - | mso-bidi-font-size:26.0pt;
| |
| - | font-family:"Cambria","serif";
| |
| - | mso-ascii-font-family:Cambria;
| |
| - | mso-ascii-theme-font:major-latin;
| |
| - | mso-fareast-font-family:"Times New Roman";
| |
| - | mso-fareast-theme-font:major-fareast;
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| - |
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| - | <div class=Section1>
| |
| - |
| |
| - | <div style='mso-element:para-border-div;border:none;border-bottom:solid #4F81BD 1.0pt;
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| - | mso-border-bottom-themecolor:accent1;padding:0cm 1cm 4.0pt 0cm'>
| |
| - |
| |
| - | <embed src="https://static.igem.org/mediawiki/2010/5/51/Nish1.swf" width=900 height=500>
| |
| - |
| |
| - | <p align=center><img src="https://static.igem.org/mediawiki/2010/5/58/IGEM_IITD_logo.png" width="100"/></p>
| |
| - |
| |
| - | <p class=MsoTitle align=center style='text-align:center'><span lang=EN-US><i style='mso-bidi-font-style:
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| - | normal'><b>Dr. Coli </b><o:p></o:p></i></span></p>
| |
| - |
| |
| - | </div>
| |
| - |
| |
| - | <p class=MsoNormal><span lang=EN-US><o:p> </o:p></span></p>
| |
| - |
| |
| - | <h2><span lang=EN-US><u>Renin Angiotensin Aldosterone System (RAAS)<o:p></o:p></span></u></h2>
| |
| - |
| |
| - | <p class=MsoNormal><span lang=EN-US><o:p> </o:p></span></p>
| |
| - |
| |
| - | <h3><span lang=EN-US>Introduction</span></h3>
| |
| - |
| |
| - | <p class=MsoNormal style='text-align:justify'><span lang=EN-US>The Renin
| |
| - | Angiotensin Aldosterone System is a hormone system in the human body that is
| |
| - | responsible for the regulation of blood pressure and fluid balance.</span></p>
| |
| - |
| |
| - | <h3 style='text-align:justify'><span lang=EN-US>Functioning</span></h3>
| |
| - |
| |
| - | <p class=MsoNormal style='text-align:justify'><span lang=EN-US>A decrease in
| |
| - | renal perfusion causes the release of the enzyme renin which subsequently
| |
| - | cleaves angiotensinogen to angiotensin I. This is further converted to
| |
| - | angiotensin II by angiotensin-converting enzyme (ACE). The bioactive product,
| |
| - | angiotensin II, causes the constriction of blood vessels, leading to an
| |
| - | increased blood pressure. It is also responsible for the secretion of
| |
| - | aldosterone by the adrenal cortex, which acts by increasing the re-absorption
| |
| - | of sodium and water into the blood. Hence, the fluid volume as well as the
| |
| - | blood pressure is maintained in the body.</span></p>
| |
| - |
| |
| - | <h2><u><span lang=EN-US>The α-hemolysin System in <i style='mso-bidi-font-style:
| |
| - | normal'>E.coli<o:p></o:p></i></span></u></h2>
| |
| - |
| |
| - | <p class=MsoNormal><span lang=EN-US><o:p> </o:p></span></p>
| |
| - |
| |
| - | <h3><span lang=EN-US>Introduction</span></h3>
| |
| - |
| |
| - | <p class=MsoNormal style='text-align:justify'><span lang=EN-US>The
| |
| - | alpha-hemolysin system is a type I secretion system found in wild type <i
| |
| - | style='mso-bidi-font-style:normal'>E.coli </i>which enable them to cause
| |
| - | infection in the urinary tract and is an important virulence factor because of
| |
| - | its cytotoxic and cytolytic activity against a variety of mammalian cells. </span></p>
| |
| - |
| |
| - | <h3 style='text-align:justify'><span lang=EN-US>Functioning</span></h3>
| |
| - |
| |
| - | <p class=MsoNormal style='text-align:justify'><span lang=EN-US>The hemolysin
| |
| - | system originates from a series of hly genes. These genes are present in the
| |
| - | series <i style='mso-bidi-font-style:normal'>hlyC – hlyA – hlyB – hlyD</i>. Of
| |
| - | these, <i style='mso-bidi-font-style:normal'>hlyA</i> codes for the protein
| |
| - | that is responsible for the infection. Hly B and D are membrane proteins that
| |
| - | work in coordination for the secretion of HlyA. The synthesis, activation and
| |
| - | secretion of HlyA in <i style='mso-bidi-font-style:normal'>E.coli </i>is
| |
| - | determined by the <i style='mso-bidi-font-style:normal'>hlyCABD</i> operon. This
| |
| - | operon is either located either on the chromosome or on transmissible plasmids.
| |
| - |
| |
| - | </span></p>
| |
| - |
| |
| - | <p class=MsoNormal style='text-align:justify'><span lang=EN-US>It has been
| |
| - | shown that this system can be used to produce and secrete a protein of our
| |
| - | choice if the protein contains 60 amino acid residues from the carboxy terminal
| |
| - | end of the HlyA protein. </span></p>
| |
| - |
| |
| - | <h2><u><span lang=EN-US>Our iGEM 2010 Project<o:p></o:p></span></u></h2>
| |
| - |
| |
| - | <p class=MsoNormal><span lang=EN-US><o:p> </o:p></span></p>
| |
| - |
| |
| - | <h3><span lang=EN-US>Introduction</span></h3>
| |
| - |
| |
| - | <p class=MsoNormal style='text-align:justify'><span lang=EN-US>Our aim is to
| |
| - | create an online elicitor detection and response in a flow system, which can
| |
| - | function in the detection of high blood pressure and help reduce it by
| |
| - | releasing renin inhibitors into the circulatory system. This kind of a system
| |
| - | would be helpful to patients with a hypertensive condition. </span></p>
| |
| - |
| |
| - | <h3><span lang=EN-US>Approach</span></h3>
| |
| - |
| |
| - | <p class=MsoNormal><span lang=EN-US>Since the blood pressure system is not
| |
| - | really a chemical signal, what we have assumed is that the blood pressure can
| |
| - | be easily converted into a chemical signal. Having assumed this, we will use a
| |
| - | chemical signal such as IPTG or AHL as the elicitor to produce appropriate
| |
| - | amounts of renin inhibitor. For easy detection and verification, we are
| |
| - | planning to use GFP in place of renin inhibitor which can easily be replaced
| |
| - | later.</span></p>
| |
| - |
| |
| - | <p class=MsoNormal><span lang=EN-US>For this we will be introducing two vectors
| |
| - | into <i style='mso-bidi-font-style:normal'>E. coli </i>as shown below.</span></p>
| |
| - |
| |
| - | <p class=MsoNormal><span style='mso-ansi-language:EN-IN;mso-fareast-language:
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| |
| - | lang=EN-US><span style='mso-spacerun:yes'> </span><span
| |
| - | style='mso-tab-count:1'> </span></span><span style='mso-ansi-language:
| |
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| |
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| |
| - |
| |
| - | <p class=MsoNormal><span lang=EN-US><o:p> </o:p></span></p>
| |
| - |
| |
| - | <p class=MsoNormal style='text-align:justify'><span lang=EN-US>The first vector
| |
| - | which contains the GFP-hlyA chimeric sequence with pLac promoter is used to
| |
| - | produce the chimera GFP protein which contains 62 amino acid residues from the
| |
| - | C terminal of hlyA protein, a prerequisite for the secretion of the protein
| |
| - | outside the cell body. Forming a chimera will not in any way change the
| |
| - | structure or function as per literature.</span></p>
| |
| - |
| |
| - | <p class=MsoNormal><span lang=EN-US><o:p> </o:p></span></p>
| |
| - |
| |
| - | <p class=MsoNormal><span lang=EN-US>The other vector contains the hlyB and hlyD
| |
| - | sequences with ptet promoter sequence. These two proteins will be
| |
| - | constitutively expressed. These proteins are present in the membrane and
| |
| - | provide for an outlet of hlyA or chimeras with 62 amino acid residues of the C
| |
| - | terminal of hlyA.</span></p>
| |
| - |
| |
| - | <p class=MsoNormal><span lang=EN-US>After we are successful in obtaining
| |
| - | GFP-hlyA chimera outside the cell body, our next task will be to immobilize
| |
| - | these cells in a flow system and characterize the flow-expression profile. We
| |
| - | would want to keep the cells in G0 phase so as to have continuous expression of
| |
| - | the protein without cell death</span></p>
| |
| - |
| |
| - | <p class=MsoNormal><span lang=EN-US>We will also be working towards minimizing
| |
| - | the toxins and other products so that such a system can finally be thought fit
| |
| - | for incorporating and integrating with the circulatory system in our body.</span></p>
| |
| - |
| |
| - | <p class=MsoNormal><span lang=EN-US><o:p> </o:p></span></p>
| |
| - |
| |
| - | <p class=MsoNormal style='text-align:justify'><span lang=EN-US><o:p> </o:p></span></p>
| |
| - |
| |
| - | </div>
| |
| - |
| |
| - | </body>
| |
| - |
| |
| - | </html>
| |
| - |
| |
| - |
| |
| - | {| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
| |
| - | !align="center"|[[Team:IIT_Delhi_1|Home]]
| |
| - | !align="center"|[[Team:IIT_Delhi_1/Team|Team]]
| |
| - | !align="center"|[https://igem.org/Team.cgi?year=2010&team_name=IIT_Delhi_1 Official Team Profile]
| |
| - | !align="center"|[[Team:IIT_Delhi_1/Project|Project]]
| |
| - | !align="center"|[[Team:IIT_Delhi_1/Parts|Parts Submitted to the Registry]]
| |
| - | !align="center"|[[Team:IIT_Delhi_1/Modeling|Modeling]]
| |
| - | !align="center"|[[Team:IIT_Delhi_1/Notebook|Notebook]]
| |
| - | !align="center"|[[Team:IIT_Delhi_1/Safety|Safety]]
| |
| - | |}
| |
| - | ''Italic text''
| |
"
