Projects

Dr. E.coli : The smallest protein injector in the world

   Our main project is on Type III Secretion Apparatus which is one of the most amazing biological devices. This apparatus which looks like a syringe can pass a whole protein molecule from a bacterial cell to a target eukaryotic cell. However, this apparatus is an organelle of pathogenic gram-negative bacterium such as Salmonella and Yersinia. So we are aiming at making this device safely available using E. coli.

Construction of a quick protocol

なんとやら

Team

The Hokkaido University's igem 2010 team, HokkaidoU_Japan consists one instructer and 7 undergrad students from facalty of Sciences, Medicine and Agriculture. This is the first year for us to participate in iGEM competition.

Parts

   We registered two parts. One is a signal sequence that can be used to secrete tagged proteins through Type 3 Secretion Apparatus. The other is whole contruct that checks whether the Type III secretion system works correctly.

Notebook

   The Notebook is a record of a day-by-day repeat of failures. The protocols that we carried out and the Materials and Methods are also here.

Safety

   なんとやら