"
Team:HokkaidoU Japan/Test2
From 2010.igem.org
(→Team) |
|||
| Line 97: | Line 97: | ||
[[Image:HokkaidoU_Japan_logo.png|thumb|200px|right|HokkaidoU Japan Logo]] | [[Image:HokkaidoU_Japan_logo.png|thumb|200px|right|HokkaidoU Japan Logo]] | ||
==Dr. ''E.coli'' : The smallest protein injector in the world== | ==Dr. ''E.coli'' : The smallest protein injector in the world== | ||
| - | + | Our main project is on Type III Secretion Apparatus which is one of the most amazing biological devices. This apparatus which looks like a syringe can pass a whole protein molecule from a bacterial cell to a target eukaryotic cell. However, this apparatus is an organelle of pathogenic gram-negative bacterium such as ''Salmonella'' and ''Yersinia''. So we are aiming at making this device safely available using ''E. coli''. | |
==Construction of a quick protocol== | ==Construction of a quick protocol== | ||
| Line 107: | Line 107: | ||
=[[Team:HokkaidoU_Japan/Parts|Parts]]= | =[[Team:HokkaidoU_Japan/Parts|Parts]]= | ||
| - | + | We registered two parts. One is a signal sequence that can be used to secrete tagged proteins through Type 3 Secretion Apparatus. The other is whole contruct that checks whether the Type III secretion system works correctly. | |
=[[Team:HokkaidoU_Japan/Notebook|Notebook]]= | =[[Team:HokkaidoU_Japan/Notebook|Notebook]]= | ||
| - | + | The [[Team:HokkaidoU_Japan/Notebook|Notebook]] is a record of a day-by-day repeat of failures. The [[Team:HokkaidoU_Japan/Protocols|protocols]] that we carried out and the [[Team:HokkaidoU_Japan/Materials_And_Methods|Materials and Methods]] are also here. | |
=[[Team:HokkaidoU_Japan/Safety|Safety]]= | =[[Team:HokkaidoU_Japan/Safety|Safety]]= | ||
| - | + | なんとやら | |
Revision as of 08:28, 26 October 2010
since 13 Jul 2010
since 1 Aug 2010
Projects
Dr. E.coli : The smallest protein injector in the world
Our main project is on Type III Secretion Apparatus which is one of the most amazing biological devices. This apparatus which looks like a syringe can pass a whole protein molecule from a bacterial cell to a target eukaryotic cell. However, this apparatus is an organelle of pathogenic gram-negative bacterium such as Salmonella and Yersinia. So we are aiming at making this device safely available using E. coli.
Construction of a quick protocol
なんとやら
Team
The Hokkaido University's igem 2010 team, HokkaidoU_Japan consists of one instructer and 7 undergrad students from facalty of Sciences, Medicine and Agriculture. This is the first year for us to participate in iGEM competition.
Parts
We registered two parts. One is a signal sequence that can be used to secrete tagged proteins through Type 3 Secretion Apparatus. The other is whole contruct that checks whether the Type III secretion system works correctly.
Notebook
The Notebook is a record of a day-by-day repeat of failures. The protocols that we carried out and the Materials and Methods are also here.
Safety
なんとやら
