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Team:HokkaidoU Japan/Test2
From 2010.igem.org
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=[[Team:HokkaidoU_Japan/Project|Projects]]= | =[[Team:HokkaidoU_Japan/Project|Projects]]= | ||
| - | <div id="project" class="homepics" style="float:right;">[[Team:HokkaidoU_Japan/Project| ]]</div> | + | <div id="project" class="homepics" style="float:right; z-index:1;">[[Team:HokkaidoU_Japan/Project| ]]</div> |
==Dr. ''E.coli'' : The smallest protein injector in the world== | ==Dr. ''E.coli'' : The smallest protein injector in the world== | ||
Our main project is on Type III Secretion Apparatus which is one of the most amazing biological devices. This apparatus which looks like a syringe can pass a whole protein molecule from a bacterial cell to a target eukaryotic cell. However, this apparatus is an organelle of pathogenic gram-negative bacterium such as ''Salmonella'' and ''Yersinia''. So we are aiming at making this device safely available using ''E. coli''. | Our main project is on Type III Secretion Apparatus which is one of the most amazing biological devices. This apparatus which looks like a syringe can pass a whole protein molecule from a bacterial cell to a target eukaryotic cell. However, this apparatus is an organelle of pathogenic gram-negative bacterium such as ''Salmonella'' and ''Yersinia''. So we are aiming at making this device safely available using ''E. coli''. | ||
Revision as of 08:07, 26 October 2010
since 13 Jul 2010
since 1 Aug 2010
Projects
Dr. E.coli : The smallest protein injector in the world
Our main project is on Type III Secretion Apparatus which is one of the most amazing biological devices. This apparatus which looks like a syringe can pass a whole protein molecule from a bacterial cell to a target eukaryotic cell. However, this apparatus is an organelle of pathogenic gram-negative bacterium such as Salmonella and Yersinia. So we are aiming at making this device safely available using E. coli.
Construction of a quick protocol
なんとやら
Team
The Hokkaido University's igem 2010 team, HokkaidoU_Japan consists one instructer and 7 undergrad students from facalty of Sciences, Medicine and Agriculture. This is the first year for us to participate in iGEM competition.
Parts
We registered two parts. One is a signal sequence that can be used to secrete tagged proteins through Type 3 Secretion Apparatus. The other is whole contruct that checks whether the Type III secretion system works correctly.
Notebook
The Notebook is a record of a day-by-day repeat of failures. The protocols that we carried out and the Materials and Methods are also here.
Safety
なんとやら
